Strain Fitness in Escherichia coli ECRC102 around NIAGMN_27635

Experiment: Sodium-DL-Lactate

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntadhE and tdk are separated by 300 nucleotidestdk and hns are separated by 603 nucleotides NIAGMN_27630: adhE - bifunctional acetaldehyde-CoA/alcohol dehydrogenase, at 5,351,626 to 5,354,301 adhE NIAGMN_27635: tdk - thymidine kinase, at 5,354,602 to 5,355,219 tdk NIAGMN_27640: hns - DNA-binding transcriptional regulator H-NS, at 5,355,823 to 5,356,236 hns Position (kb) 5354 5355 5356Strain fitness (log2 ratio) -2 -1 0 1at 5353.787 kb on + strand, within adhEat 5353.788 kb on - strand, within adhEat 5353.948 kb on + strand, within adhEat 5354.212 kb on + strandat 5354.224 kb on + strandat 5354.224 kb on + strandat 5354.225 kb on - strandat 5354.324 kb on + strandat 5354.416 kb on + strandat 5354.730 kb on - strand, within tdkat 5355.114 kb on - strand, within tdkat 5355.116 kb on - strand, within tdkat 5355.264 kb on + strandat 5355.403 kb on + strandat 5355.436 kb on + strandat 5355.652 kb on + strandat 5356.164 kb on + strand, within hns

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Per-strain Table

Position Strand Gene LocusTag Fraction Sodium-DL-Lactate
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5,353,787 + adhE NIAGMN_27630 0.81 +0.0
5,353,788 - adhE NIAGMN_27630 0.81 -0.1
5,353,948 + adhE NIAGMN_27630 0.87 -0.5
5,354,212 + +0.0
5,354,224 + -1.3
5,354,224 + -1.5
5,354,225 - +0.0
5,354,324 + -0.1
5,354,416 + -2.3
5,354,730 - tdk NIAGMN_27635 0.21 +1.6
5,355,114 - tdk NIAGMN_27635 0.83 -0.4
5,355,116 - tdk NIAGMN_27635 0.83 -0.3
5,355,264 + +0.7
5,355,403 + +0.2
5,355,436 + +1.6
5,355,652 + -0.4
5,356,164 + hns NIAGMN_27640 0.82 -1.0

Or see this region's nucleotide sequence