Experiment: Sodium-DL-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt NIAGMN_27035 and rusA overlap by 4 nucleotides rusA and NIAGMN_27045 are separated by 12 nucleotides NIAGMN_27045 and NIAGMN_27050 are separated by 345 nucleotides NIAGMN_27050 and NIAGMN_27055 are separated by 278 nucleotides
NIAGMN_27035: NIAGMN_27035 - antitermination protein, at 5,261,106 to 5,261,927
_27035
NIAGMN_27040: rusA - Endodeoxyribonuclease RusA, at 5,261,924 to 5,262,298
rusA
NIAGMN_27045: NIAGMN_27045 - DUF968 domain-containing protein, at 5,262,311 to 5,263,360
_27045
NIAGMN_27050: NIAGMN_27050 - hypothetical protein, at 5,263,706 to 5,263,873
_27050
NIAGMN_27055: NIAGMN_27055 - protein hokC, at 5,264,152 to 5,264,364
_27055
Position (kb) 5262
5263
5264 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 5262.003 kb on - strand, within rusA at 5263.645 kb on - strand at 5263.689 kb on + strand at 5263.693 kb on - strand at 5263.700 kb on + strand at 5263.708 kb on - strand at 5263.729 kb on - strand, within NIAGMN_27050 at 5263.769 kb on - strand, within NIAGMN_27050 at 5263.828 kb on - strand, within NIAGMN_27050 at 5263.892 kb on + strand at 5263.927 kb on - strand at 5263.944 kb on - strand at 5263.944 kb on - strand at 5264.027 kb on - strand at 5264.027 kb on - strand at 5264.057 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Sodium-DL-Lactate remove 5,262,003 - rusA NIAGMN_27040 0.21 +0.6 5,263,645 - -0.7 5,263,689 + +0.8 5,263,693 - +0.3 5,263,700 + -2.8 5,263,708 - -0.5 5,263,729 - NIAGMN_27050 0.14 -0.4 5,263,769 - NIAGMN_27050 0.38 -0.4 5,263,828 - NIAGMN_27050 0.73 +0.6 5,263,892 + -2.1 5,263,927 - -4.4 5,263,944 - +2.9 5,263,944 - -0.4 5,264,027 - -0.8 5,264,027 - +0.5 5,264,057 - -2.5
Or see this region's nucleotide sequence