Strain Fitness in Escherichia coli ECRC102 around NIAGMN_26615

Experiment: Sodium-DL-Lactate

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntpuuD and puuA are separated by 211 nucleotidespuuA and puuP are separated by 302 nucleotides NIAGMN_26610: puuD - gamma-glutamyl-gamma-aminobutyrate hydrolase, at 5,176,694 to 5,177,458 puuD NIAGMN_26615: puuA - glutamate-putrescine ligase, at 5,177,670 to 5,179,088 puuA NIAGMN_26620: puuP - putrescine/proton symporter PuuP, at 5,179,391 to 5,180,776 puuP Position (kb) 5177 5178 5179 5180Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3at 5176.802 kb on + strand, within puuDat 5176.848 kb on - strand, within puuDat 5177.420 kb on + strandat 5177.964 kb on - strand, within puuAat 5178.156 kb on + strand, within puuAat 5178.536 kb on + strand, within puuAat 5178.687 kb on + strand, within puuAat 5178.687 kb on + strand, within puuAat 5178.716 kb on + strand, within puuAat 5178.741 kb on - strand, within puuAat 5178.979 kb on + strandat 5179.086 kb on + strandat 5179.566 kb on - strand, within puuP

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Per-strain Table

Position Strand Gene LocusTag Fraction Sodium-DL-Lactate
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5,176,802 + puuD NIAGMN_26610 0.14 -0.4
5,176,848 - puuD NIAGMN_26610 0.20 +0.8
5,177,420 + +1.6
5,177,964 - puuA NIAGMN_26615 0.21 -0.2
5,178,156 + puuA NIAGMN_26615 0.34 +1.2
5,178,536 + puuA NIAGMN_26615 0.61 +2.9
5,178,687 + puuA NIAGMN_26615 0.72 +1.8
5,178,687 + puuA NIAGMN_26615 0.72 +1.9
5,178,716 + puuA NIAGMN_26615 0.74 -0.1
5,178,741 - puuA NIAGMN_26615 0.75 +0.4
5,178,979 + -0.3
5,179,086 + -4.3
5,179,566 - puuP NIAGMN_26620 0.13 -3.9

Or see this region's nucleotide sequence