Experiment: Sodium-DL-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hyaC and hyaD overlap by 4 nucleotides hyaD and NIAGMN_23060 overlap by 4 nucleotides NIAGMN_23060 and NIAGMN_23065 overlap by 4 nucleotides NIAGMN_23065 and appC are separated by 133 nucleotides
NIAGMN_23050: hyaC - Ni/Fe-hydrogenase b-type cytochrome subunit, at 4,535,491 to 4,536,198
hyaC
NIAGMN_23055: hyaD - hydrogenase 1 maturation protease, at 4,536,195 to 4,536,782
hyaD
NIAGMN_23060: NIAGMN_23060 - hydrogenase 1 maturation protease, at 4,536,779 to 4,537,177
_23060
NIAGMN_23065: NIAGMN_23065 - hydrogenase expression/formation protein, at 4,537,174 to 4,538,031
_23065
NIAGMN_23070: appC - cytochrome bd-II oxidase subunit 1, at 4,538,165 to 4,539,709
appC
Position (kb) 4537
4538
4539 Strain fitness (log2 ratio) -2
-1
0
1
2 at 4536.176 kb on + strand at 4536.217 kb on + strand at 4536.217 kb on + strand at 4536.282 kb on + strand, within hyaD at 4536.430 kb on - strand, within hyaD at 4536.454 kb on - strand, within hyaD at 4536.684 kb on - strand, within hyaD at 4536.705 kb on + strand, within hyaD at 4536.705 kb on + strand, within hyaD at 4536.980 kb on + strand, within NIAGMN_23060 at 4537.840 kb on + strand, within NIAGMN_23065 at 4537.841 kb on - strand, within NIAGMN_23065 at 4537.877 kb on + strand, within NIAGMN_23065 at 4537.877 kb on + strand, within NIAGMN_23065 at 4537.877 kb on + strand, within NIAGMN_23065 at 4537.877 kb on + strand, within NIAGMN_23065 at 4537.895 kb on + strand, within NIAGMN_23065 at 4537.997 kb on + strand at 4537.997 kb on + strand at 4538.029 kb on + strand at 4538.059 kb on + strand at 4538.401 kb on + strand, within appC at 4538.423 kb on + strand, within appC at 4538.423 kb on + strand, within appC at 4538.423 kb on + strand, within appC at 4538.423 kb on + strand, within appC at 4538.535 kb on - strand, within appC at 4538.758 kb on + strand, within appC at 4538.759 kb on - strand, within appC
Per-strain Table
Position Strand Gene LocusTag Fraction Sodium-DL-Lactate remove 4,536,176 + -0.3 4,536,217 + -2.7 4,536,217 + -0.9 4,536,282 + hyaD NIAGMN_23055 0.15 +0.5 4,536,430 - hyaD NIAGMN_23055 0.40 +0.2 4,536,454 - hyaD NIAGMN_23055 0.44 +1.3 4,536,684 - hyaD NIAGMN_23055 0.83 +1.0 4,536,705 + hyaD NIAGMN_23055 0.87 +0.9 4,536,705 + hyaD NIAGMN_23055 0.87 -2.0 4,536,980 + NIAGMN_23060 0.50 -0.6 4,537,840 + NIAGMN_23065 0.78 +1.7 4,537,841 - NIAGMN_23065 0.78 +0.6 4,537,877 + NIAGMN_23065 0.82 -0.1 4,537,877 + NIAGMN_23065 0.82 +0.5 4,537,877 + NIAGMN_23065 0.82 +1.7 4,537,877 + NIAGMN_23065 0.82 -2.6 4,537,895 + NIAGMN_23065 0.84 -1.2 4,537,997 + -0.5 4,537,997 + -0.8 4,538,029 + +0.1 4,538,059 + -1.0 4,538,401 + appC NIAGMN_23070 0.15 -0.4 4,538,423 + appC NIAGMN_23070 0.17 +0.2 4,538,423 + appC NIAGMN_23070 0.17 -1.9 4,538,423 + appC NIAGMN_23070 0.17 -0.1 4,538,423 + appC NIAGMN_23070 0.17 +0.6 4,538,535 - appC NIAGMN_23070 0.24 +0.8 4,538,758 + appC NIAGMN_23070 0.38 +2.5 4,538,759 - appC NIAGMN_23070 0.38 -0.7
Or see this region's nucleotide sequence