Experiment: Sodium-DL-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt eco and NIAGMN_21000 are separated by 133 nucleotides NIAGMN_21000 and NIAGMN_21005 are separated by 51 nucleotides NIAGMN_21005 and napF are separated by 15 nucleotides napF and napD overlap by 11 nucleotides napD and napA overlap by 4 nucleotides
NIAGMN_20995: eco - serine protease inhibitor ecotin, at 4,162,413 to 4,162,901
eco
NIAGMN_21000: NIAGMN_21000 - hypothetical protein, at 4,163,035 to 4,163,199
_21000
NIAGMN_21005: NIAGMN_21005 - Orf 5, at 4,163,251 to 4,163,292
_21005
NIAGMN_21010: napF - ferredoxin-type protein NapF, at 4,163,308 to 4,163,802
napF
NIAGMN_21015: napD - chaperone NapD, at 4,163,792 to 4,164,055
napD
NIAGMN_21020: napA - nitrate reductase catalytic subunit NapA, at 4,164,052 to 4,166,538
napA
Position (kb) 4163
4164 Strain fitness (log2 ratio) -2
-1
0
1
2 at 4162.352 kb on - strand at 4162.423 kb on - strand at 4162.473 kb on + strand, within eco at 4162.474 kb on - strand, within eco at 4162.474 kb on - strand, within eco at 4162.474 kb on - strand, within eco at 4162.474 kb on - strand, within eco at 4162.488 kb on - strand, within eco at 4162.490 kb on - strand, within eco at 4162.490 kb on - strand, within eco at 4162.517 kb on - strand, within eco at 4162.611 kb on - strand, within eco at 4162.628 kb on - strand, within eco at 4162.628 kb on - strand, within eco at 4162.631 kb on - strand, within eco at 4162.631 kb on - strand, within eco at 4162.631 kb on - strand, within eco at 4162.740 kb on - strand, within eco at 4162.926 kb on - strand at 4162.961 kb on - strand at 4162.961 kb on - strand at 4162.993 kb on - strand at 4163.068 kb on + strand, within NIAGMN_21000 at 4163.094 kb on - strand, within NIAGMN_21000 at 4163.215 kb on + strand at 4163.215 kb on + strand at 4163.218 kb on + strand at 4163.219 kb on - strand at 4163.247 kb on - strand at 4163.437 kb on - strand, within napF at 4163.439 kb on + strand, within napF at 4163.556 kb on - strand, within napF at 4163.652 kb on + strand, within napF at 4163.652 kb on + strand, within napF at 4163.674 kb on - strand, within napF at 4163.701 kb on + strand, within napF at 4163.716 kb on - strand, within napF at 4163.800 kb on + strand at 4163.801 kb on - strand at 4163.801 kb on - strand at 4164.297 kb on + strand at 4164.326 kb on - strand, within napA at 4164.360 kb on + strand, within napA at 4164.360 kb on + strand, within napA at 4164.372 kb on + strand, within napA at 4164.373 kb on - strand, within napA at 4164.377 kb on - strand, within napA at 4164.588 kb on + strand, within napA at 4164.628 kb on - strand, within napA
Per-strain Table
Position Strand Gene LocusTag Fraction Sodium-DL-Lactate remove 4,162,352 - +1.9 4,162,423 - +0.5 4,162,473 + eco NIAGMN_20995 0.12 +1.0 4,162,474 - eco NIAGMN_20995 0.12 -1.6 4,162,474 - eco NIAGMN_20995 0.12 -0.7 4,162,474 - eco NIAGMN_20995 0.12 -0.4 4,162,474 - eco NIAGMN_20995 0.12 -0.1 4,162,488 - eco NIAGMN_20995 0.15 +0.4 4,162,490 - eco NIAGMN_20995 0.16 +0.9 4,162,490 - eco NIAGMN_20995 0.16 +1.7 4,162,517 - eco NIAGMN_20995 0.21 +0.7 4,162,611 - eco NIAGMN_20995 0.40 +0.2 4,162,628 - eco NIAGMN_20995 0.44 -0.4 4,162,628 - eco NIAGMN_20995 0.44 +1.1 4,162,631 - eco NIAGMN_20995 0.45 +0.1 4,162,631 - eco NIAGMN_20995 0.45 -0.3 4,162,631 - eco NIAGMN_20995 0.45 +0.6 4,162,740 - eco NIAGMN_20995 0.67 -1.4 4,162,926 - +0.1 4,162,961 - -1.7 4,162,961 - -1.1 4,162,993 - +0.9 4,163,068 + NIAGMN_21000 0.20 -0.8 4,163,094 - NIAGMN_21000 0.36 -0.4 4,163,215 + +0.1 4,163,215 + -1.0 4,163,218 + +1.8 4,163,219 - +0.0 4,163,247 - +0.6 4,163,437 - napF NIAGMN_21010 0.26 +1.8 4,163,439 + napF NIAGMN_21010 0.26 +1.8 4,163,556 - napF NIAGMN_21010 0.50 -1.4 4,163,652 + napF NIAGMN_21010 0.69 -0.2 4,163,652 + napF NIAGMN_21010 0.69 -0.1 4,163,674 - napF NIAGMN_21010 0.74 -0.6 4,163,701 + napF NIAGMN_21010 0.79 -0.2 4,163,716 - napF NIAGMN_21010 0.82 -0.6 4,163,800 + -2.1 4,163,801 - +1.0 4,163,801 - +1.7 4,164,297 + +0.7 4,164,326 - napA NIAGMN_21020 0.11 -1.1 4,164,360 + napA NIAGMN_21020 0.12 +1.6 4,164,360 + napA NIAGMN_21020 0.12 +0.2 4,164,372 + napA NIAGMN_21020 0.13 -1.4 4,164,373 - napA NIAGMN_21020 0.13 -0.5 4,164,377 - napA NIAGMN_21020 0.13 -0.1 4,164,588 + napA NIAGMN_21020 0.22 -0.1 4,164,628 - napA NIAGMN_21020 0.23 -0.3
Or see this region's nucleotide sequence