Strain Fitness in Escherichia coli ECRC102 around NIAGMN_20900

Experiment: Sodium-DL-Lactate

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntubiG and gyrA are separated by 146 nucleotidesgyrA and yfaA are separated by 282 nucleotides NIAGMN_20895: ubiG - bifunctional 2-polyprenyl-6-hydroxyphenol methylase/3-demethylubiquinol 3-O-methyltransferase UbiG, at 4,134,299 to 4,135,021 ubiG NIAGMN_20900: gyrA - DNA topoisomerase (ATP-hydrolyzing) subunit A, at 4,135,168 to 4,137,795 gyrA NIAGMN_20905: yfaA - DUF2138 domain-containing protein, at 4,138,078 to 4,139,631 yfaA Position (kb) 4135 4136 4137 4138Strain fitness (log2 ratio) -2 -1 0 1at 4134.177 kb on + strandat 4134.228 kb on - strandat 4134.305 kb on - strandat 4135.032 kb on - strandat 4135.059 kb on - strandat 4135.059 kb on - strandat 4135.059 kb on - strandat 4135.060 kb on + strandat 4135.060 kb on + strandat 4135.060 kb on + strandat 4135.061 kb on - strandat 4135.080 kb on + strandat 4135.081 kb on - strandat 4135.089 kb on + strandat 4135.090 kb on - strandat 4135.096 kb on - strandat 4137.793 kb on + strandat 4137.799 kb on + strandat 4138.213 kb on + strandat 4138.372 kb on + strand, within yfaAat 4138.372 kb on + strand, within yfaAat 4138.373 kb on - strand, within yfaAat 4138.494 kb on + strand, within yfaAat 4138.549 kb on + strand, within yfaA

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Per-strain Table

Position Strand Gene LocusTag Fraction Sodium-DL-Lactate
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4,134,177 + +1.0
4,134,228 - +0.3
4,134,305 - +0.2
4,135,032 - -0.8
4,135,059 - +0.0
4,135,059 - +0.8
4,135,059 - +1.2
4,135,060 + -1.2
4,135,060 + +0.7
4,135,060 + -1.6
4,135,061 - -0.6
4,135,080 + -0.8
4,135,081 - -0.1
4,135,089 + -1.3
4,135,090 - +0.8
4,135,096 - +0.7
4,137,793 + +0.8
4,137,799 + -1.1
4,138,213 + -0.8
4,138,372 + yfaA NIAGMN_20905 0.19 +1.7
4,138,372 + yfaA NIAGMN_20905 0.19 -1.8
4,138,373 - yfaA NIAGMN_20905 0.19 +0.8
4,138,494 + yfaA NIAGMN_20905 0.27 -1.6
4,138,549 + yfaA NIAGMN_20905 0.30 -0.6

Or see this region's nucleotide sequence