Experiment: Sodium-DL-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nfeR and nfeF are separated by 287 nucleotides nfeF and Ile2_trna are separated by 53 nucleotides Ile2_trna and mug are separated by 124 nucleotides mug and rpoD are separated by 78 nucleotides
NIAGMN_16400: nfeR - DNA-binding transcriptional regulator NfeR, at 3,244,796 to 3,245,419
nfeR
NIAGMN_16405: nfeF - NADPH-dependent ferric chelate reductase, at 3,245,707 to 3,246,471
nfeF
NIAGMN_16410: Ile2_trna - tRNA-Ile2(cat), at 3,246,525 to 3,246,600
_trna
NIAGMN_16415: mug - G/U mismatch-specific DNA glycosylase, at 3,246,725 to 3,247,231
mug
NIAGMN_16420: rpoD - RNA polymerase sigma factor RpoD, at 3,247,310 to 3,249,151
rpoD
Position (kb)
3245
3246
3247 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3244.720 kb on + strand at 3244.730 kb on - strand at 3244.730 kb on - strand at 3244.730 kb on - strand at 3244.797 kb on + strand at 3244.811 kb on - strand at 3244.814 kb on - strand at 3244.913 kb on - strand, within nfeR at 3244.913 kb on - strand, within nfeR at 3245.057 kb on - strand, within nfeR at 3245.057 kb on - strand, within nfeR at 3245.080 kb on + strand, within nfeR at 3245.143 kb on - strand, within nfeR at 3245.143 kb on - strand, within nfeR at 3245.174 kb on - strand, within nfeR at 3245.393 kb on - strand at 3245.522 kb on - strand at 3245.560 kb on + strand at 3245.560 kb on + strand at 3245.560 kb on + strand at 3245.560 kb on + strand at 3245.560 kb on + strand at 3245.560 kb on + strand at 3245.561 kb on - strand at 3245.561 kb on - strand at 3245.561 kb on - strand at 3245.561 kb on - strand at 3245.561 kb on - strand at 3245.561 kb on - strand at 3245.601 kb on - strand at 3245.606 kb on + strand at 3245.615 kb on + strand at 3245.616 kb on - strand at 3245.616 kb on - strand at 3245.632 kb on + strand at 3245.712 kb on + strand at 3245.712 kb on + strand at 3245.713 kb on - strand at 3245.775 kb on - strand at 3245.841 kb on + strand, within nfeF at 3245.842 kb on - strand, within nfeF at 3245.842 kb on - strand, within nfeF at 3245.968 kb on + strand, within nfeF at 3245.969 kb on - strand, within nfeF at 3245.970 kb on + strand, within nfeF at 3245.971 kb on - strand, within nfeF at 3245.990 kb on + strand, within nfeF at 3245.991 kb on - strand, within nfeF at 3245.991 kb on - strand, within nfeF at 3245.991 kb on - strand, within nfeF at 3246.072 kb on + strand, within nfeF at 3246.078 kb on + strand, within nfeF at 3246.079 kb on - strand, within nfeF at 3246.127 kb on + strand, within nfeF at 3246.128 kb on - strand, within nfeF at 3246.136 kb on + strand, within nfeF at 3246.136 kb on + strand, within nfeF at 3246.136 kb on + strand, within nfeF at 3246.136 kb on + strand, within nfeF at 3246.136 kb on + strand, within nfeF at 3246.137 kb on - strand, within nfeF at 3246.137 kb on - strand, within nfeF at 3246.137 kb on - strand, within nfeF at 3246.137 kb on - strand, within nfeF at 3246.216 kb on + strand, within nfeF at 3246.216 kb on + strand, within nfeF at 3246.217 kb on - strand, within nfeF at 3246.280 kb on - strand, within nfeF at 3246.355 kb on + strand, within nfeF at 3246.355 kb on + strand, within nfeF at 3246.356 kb on - strand, within nfeF at 3246.356 kb on - strand, within nfeF at 3246.381 kb on + strand, within nfeF at 3246.425 kb on - strand at 3246.519 kb on + strand at 3246.520 kb on - strand at 3246.520 kb on - strand at 3246.520 kb on - strand at 3246.520 kb on - strand at 3246.520 kb on - strand at 3246.520 kb on - strand at 3246.520 kb on - strand at 3246.616 kb on - strand at 3246.616 kb on - strand at 3246.616 kb on - strand at 3246.620 kb on + strand at 3246.620 kb on + strand at 3246.620 kb on + strand at 3246.691 kb on - strand at 3246.710 kb on + strand at 3246.710 kb on + strand at 3246.711 kb on - strand at 3246.711 kb on - strand at 3246.720 kb on + strand at 3246.720 kb on + strand at 3246.721 kb on - strand at 3246.724 kb on + strand at 3246.724 kb on + strand at 3246.846 kb on + strand, within mug at 3246.847 kb on - strand, within mug at 3246.847 kb on - strand, within mug at 3246.848 kb on + strand, within mug at 3246.848 kb on + strand, within mug at 3246.849 kb on - strand, within mug at 3246.849 kb on - strand, within mug at 3246.978 kb on + strand, within mug at 3246.978 kb on + strand, within mug at 3247.058 kb on + strand, within mug
Per-strain Table
Position Strand Gene LocusTag Fraction Sodium-DL-Lactate remove 3,244,720 + +1.1 3,244,730 - -0.6 3,244,730 - +0.2 3,244,730 - +1.0 3,244,797 + +2.3 3,244,811 - -0.7 3,244,814 - -1.3 3,244,913 - nfeR NIAGMN_16400 0.19 +0.6 3,244,913 - nfeR NIAGMN_16400 0.19 -2.5 3,245,057 - nfeR NIAGMN_16400 0.42 -0.2 3,245,057 - nfeR NIAGMN_16400 0.42 -0.6 3,245,080 + nfeR NIAGMN_16400 0.46 +0.9 3,245,143 - nfeR NIAGMN_16400 0.56 -1.0 3,245,143 - nfeR NIAGMN_16400 0.56 +0.2 3,245,174 - nfeR NIAGMN_16400 0.61 -0.4 3,245,393 - -0.5 3,245,522 - +0.1 3,245,560 + -2.0 3,245,560 + +0.5 3,245,560 + +0.2 3,245,560 + +0.1 3,245,560 + -0.2 3,245,560 + +0.6 3,245,561 - -0.2 3,245,561 - -0.7 3,245,561 - +2.4 3,245,561 - +0.8 3,245,561 - +0.6 3,245,561 - -1.3 3,245,601 - +2.0 3,245,606 + +2.7 3,245,615 + +0.6 3,245,616 - -0.6 3,245,616 - -1.3 3,245,632 + +1.3 3,245,712 + +0.2 3,245,712 + +0.6 3,245,713 - +1.0 3,245,775 - -1.0 3,245,841 + nfeF NIAGMN_16405 0.18 -0.0 3,245,842 - nfeF NIAGMN_16405 0.18 +0.9 3,245,842 - nfeF NIAGMN_16405 0.18 +0.4 3,245,968 + nfeF NIAGMN_16405 0.34 -0.0 3,245,969 - nfeF NIAGMN_16405 0.34 -0.5 3,245,970 + nfeF NIAGMN_16405 0.34 +0.6 3,245,971 - nfeF NIAGMN_16405 0.35 +0.5 3,245,990 + nfeF NIAGMN_16405 0.37 +0.9 3,245,991 - nfeF NIAGMN_16405 0.37 -0.9 3,245,991 - nfeF NIAGMN_16405 0.37 -0.5 3,245,991 - nfeF NIAGMN_16405 0.37 -0.6 3,246,072 + nfeF NIAGMN_16405 0.48 -2.0 3,246,078 + nfeF NIAGMN_16405 0.48 -2.2 3,246,079 - nfeF NIAGMN_16405 0.49 +0.1 3,246,127 + nfeF NIAGMN_16405 0.55 -0.1 3,246,128 - nfeF NIAGMN_16405 0.55 +1.5 3,246,136 + nfeF NIAGMN_16405 0.56 -1.0 3,246,136 + nfeF NIAGMN_16405 0.56 -0.2 3,246,136 + nfeF NIAGMN_16405 0.56 -0.5 3,246,136 + nfeF NIAGMN_16405 0.56 +0.0 3,246,136 + nfeF NIAGMN_16405 0.56 -1.3 3,246,137 - nfeF NIAGMN_16405 0.56 -0.2 3,246,137 - nfeF NIAGMN_16405 0.56 +0.9 3,246,137 - nfeF NIAGMN_16405 0.56 +0.1 3,246,137 - nfeF NIAGMN_16405 0.56 -0.6 3,246,216 + nfeF NIAGMN_16405 0.67 +1.2 3,246,216 + nfeF NIAGMN_16405 0.67 +0.5 3,246,217 - nfeF NIAGMN_16405 0.67 -1.9 3,246,280 - nfeF NIAGMN_16405 0.75 +0.9 3,246,355 + nfeF NIAGMN_16405 0.85 +2.2 3,246,355 + nfeF NIAGMN_16405 0.85 +0.7 3,246,356 - nfeF NIAGMN_16405 0.85 +0.4 3,246,356 - nfeF NIAGMN_16405 0.85 -0.2 3,246,381 + nfeF NIAGMN_16405 0.88 -0.2 3,246,425 - -0.6 3,246,519 + -0.6 3,246,520 - -0.2 3,246,520 - -0.3 3,246,520 - -0.9 3,246,520 - -0.6 3,246,520 - -0.2 3,246,520 - +0.1 3,246,520 - -0.2 3,246,616 - +0.8 3,246,616 - +0.2 3,246,616 - +0.6 3,246,620 + -0.3 3,246,620 + +0.3 3,246,620 + -1.0 3,246,691 - +0.3 3,246,710 + +0.3 3,246,710 + +0.1 3,246,711 - -0.4 3,246,711 - -0.6 3,246,720 + +0.1 3,246,720 + +0.3 3,246,721 - +0.8 3,246,724 + -1.5 3,246,724 + -0.3 3,246,846 + mug NIAGMN_16415 0.24 -0.4 3,246,847 - mug NIAGMN_16415 0.24 -1.4 3,246,847 - mug NIAGMN_16415 0.24 -3.1 3,246,848 + mug NIAGMN_16415 0.24 +1.2 3,246,848 + mug NIAGMN_16415 0.24 -0.1 3,246,849 - mug NIAGMN_16415 0.24 +0.4 3,246,849 - mug NIAGMN_16415 0.24 +0.1 3,246,978 + mug NIAGMN_16415 0.50 +0.0 3,246,978 + mug NIAGMN_16415 0.50 +0.6 3,247,058 + mug NIAGMN_16415 0.66 +0.5
Or see this region's nucleotide sequence