Experiment: Sodium-DL-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt lpoA and rsmI are separated by 64 nucleotides rsmI and NIAGMN_16000 are separated by 42 nucleotides NIAGMN_16000 and tnp-IS3 overlap by 35 nucleotides tnp-IS3 and tnp-IS3 overlap by 1 nucleotides
NIAGMN_15990: lpoA - penicillin-binding protein activator LpoA, at 3,163,795 to 3,165,831
lpoA
NIAGMN_15995: rsmI - 16S rRNA (cytidine(1402)-2'-O)-methyltransferase, at 3,165,896 to 3,166,756
rsmI
NIAGMN_16000: NIAGMN_16000 - fimbrial protein, at 3,166,799 to 3,167,290
_16000
NIAGMN_16005: tnp-IS3 - IS3 family transposase, at 3,167,256 to 3,168,143
tnp-IS3
NIAGMN_16010: tnp-IS3 - IS3 family transposase, at 3,168,143 to 3,168,469
tnp-IS3
Position (kb)
3166
3167
3168 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3165.826 kb on + strand at 3165.831 kb on + strand at 3165.831 kb on + strand at 3165.831 kb on + strand at 3165.831 kb on + strand at 3165.873 kb on - strand at 3165.873 kb on - strand at 3165.884 kb on - strand at 3165.938 kb on + strand at 3165.939 kb on - strand at 3165.945 kb on + strand at 3165.946 kb on - strand at 3165.996 kb on + strand, within rsmI at 3165.996 kb on + strand, within rsmI at 3165.996 kb on + strand, within rsmI at 3165.999 kb on + strand, within rsmI at 3166.030 kb on + strand, within rsmI at 3166.048 kb on - strand, within rsmI at 3166.069 kb on + strand, within rsmI at 3166.069 kb on + strand, within rsmI at 3166.069 kb on + strand, within rsmI at 3166.270 kb on + strand, within rsmI at 3166.329 kb on + strand, within rsmI at 3166.362 kb on + strand, within rsmI at 3166.400 kb on + strand, within rsmI at 3166.438 kb on + strand, within rsmI at 3166.449 kb on + strand, within rsmI at 3166.600 kb on + strand, within rsmI at 3166.601 kb on - strand, within rsmI at 3166.653 kb on + strand, within rsmI at 3166.653 kb on + strand, within rsmI at 3166.653 kb on + strand, within rsmI at 3166.653 kb on + strand, within rsmI at 3166.654 kb on - strand, within rsmI at 3166.727 kb on + strand at 3166.728 kb on - strand at 3166.729 kb on + strand at 3166.729 kb on + strand at 3166.729 kb on + strand at 3166.729 kb on + strand at 3166.729 kb on + strand at 3166.729 kb on + strand at 3166.729 kb on + strand at 3166.729 kb on + strand at 3166.729 kb on + strand at 3166.730 kb on - strand at 3166.730 kb on - strand at 3166.730 kb on - strand at 3166.733 kb on + strand at 3166.733 kb on + strand at 3166.734 kb on - strand at 3166.755 kb on - strand at 3166.870 kb on + strand, within NIAGMN_16000 at 3166.870 kb on + strand, within NIAGMN_16000 at 3166.927 kb on + strand, within NIAGMN_16000 at 3167.039 kb on + strand, within NIAGMN_16000 at 3167.040 kb on - strand, within NIAGMN_16000 at 3167.040 kb on - strand, within NIAGMN_16000 at 3167.095 kb on + strand, within NIAGMN_16000 at 3167.096 kb on - strand, within NIAGMN_16000 at 3167.109 kb on + strand, within NIAGMN_16000 at 3167.109 kb on + strand, within NIAGMN_16000 at 3167.110 kb on - strand, within NIAGMN_16000 at 3167.110 kb on - strand, within NIAGMN_16000 at 3167.110 kb on - strand, within NIAGMN_16000 at 3167.110 kb on - strand, within NIAGMN_16000 at 3167.110 kb on - strand, within NIAGMN_16000 at 3167.127 kb on - strand, within NIAGMN_16000 at 3167.134 kb on + strand, within NIAGMN_16000 at 3167.134 kb on + strand, within NIAGMN_16000 at 3167.135 kb on - strand, within NIAGMN_16000 at 3167.164 kb on + strand, within NIAGMN_16000 at 3167.195 kb on - strand, within NIAGMN_16000 at 3167.200 kb on + strand, within NIAGMN_16000 at 3167.200 kb on + strand, within NIAGMN_16000 at 3167.201 kb on - strand, within NIAGMN_16000 at 3167.201 kb on - strand, within NIAGMN_16000 at 3167.203 kb on + strand, within NIAGMN_16000 at 3167.203 kb on + strand, within NIAGMN_16000 at 3167.204 kb on - strand, within NIAGMN_16000 at 3167.204 kb on - strand, within NIAGMN_16000 at 3167.204 kb on - strand, within NIAGMN_16000 at 3167.204 kb on - strand, within NIAGMN_16000 at 3167.246 kb on - strand at 3167.246 kb on - strand at 3167.246 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Sodium-DL-Lactate remove 3,165,826 + -0.3 3,165,831 + -1.1 3,165,831 + -0.4 3,165,831 + -0.1 3,165,831 + +0.1 3,165,873 - -3.5 3,165,873 - +0.1 3,165,884 - +0.8 3,165,938 + -1.4 3,165,939 - +2.1 3,165,945 + -0.7 3,165,946 - +1.7 3,165,996 + rsmI NIAGMN_15995 0.12 +1.6 3,165,996 + rsmI NIAGMN_15995 0.12 -0.6 3,165,996 + rsmI NIAGMN_15995 0.12 -0.6 3,165,999 + rsmI NIAGMN_15995 0.12 +0.6 3,166,030 + rsmI NIAGMN_15995 0.16 +1.4 3,166,048 - rsmI NIAGMN_15995 0.18 -0.4 3,166,069 + rsmI NIAGMN_15995 0.20 +0.4 3,166,069 + rsmI NIAGMN_15995 0.20 -0.2 3,166,069 + rsmI NIAGMN_15995 0.20 -0.5 3,166,270 + rsmI NIAGMN_15995 0.43 -0.6 3,166,329 + rsmI NIAGMN_15995 0.50 +2.4 3,166,362 + rsmI NIAGMN_15995 0.54 -0.1 3,166,400 + rsmI NIAGMN_15995 0.59 +0.2 3,166,438 + rsmI NIAGMN_15995 0.63 -1.8 3,166,449 + rsmI NIAGMN_15995 0.64 -0.2 3,166,600 + rsmI NIAGMN_15995 0.82 +0.3 3,166,601 - rsmI NIAGMN_15995 0.82 +2.0 3,166,653 + rsmI NIAGMN_15995 0.88 +0.0 3,166,653 + rsmI NIAGMN_15995 0.88 +0.5 3,166,653 + rsmI NIAGMN_15995 0.88 +1.6 3,166,653 + rsmI NIAGMN_15995 0.88 +1.1 3,166,654 - rsmI NIAGMN_15995 0.88 -2.4 3,166,727 + -0.4 3,166,728 - -0.1 3,166,729 + -1.7 3,166,729 + +1.9 3,166,729 + +0.4 3,166,729 + -2.6 3,166,729 + +0.9 3,166,729 + +0.1 3,166,729 + -0.2 3,166,729 + -0.2 3,166,729 + -0.6 3,166,730 - -2.2 3,166,730 - -1.9 3,166,730 - +1.0 3,166,733 + +0.0 3,166,733 + -2.8 3,166,734 - -1.2 3,166,755 - +0.0 3,166,870 + NIAGMN_16000 0.14 -0.4 3,166,870 + NIAGMN_16000 0.14 -1.0 3,166,927 + NIAGMN_16000 0.26 -1.3 3,167,039 + NIAGMN_16000 0.49 +0.1 3,167,040 - NIAGMN_16000 0.49 -1.7 3,167,040 - NIAGMN_16000 0.49 +0.4 3,167,095 + NIAGMN_16000 0.60 +0.9 3,167,096 - NIAGMN_16000 0.60 +0.4 3,167,109 + NIAGMN_16000 0.63 -0.8 3,167,109 + NIAGMN_16000 0.63 +0.4 3,167,110 - NIAGMN_16000 0.63 +1.9 3,167,110 - NIAGMN_16000 0.63 -0.2 3,167,110 - NIAGMN_16000 0.63 +0.9 3,167,110 - NIAGMN_16000 0.63 +0.0 3,167,110 - NIAGMN_16000 0.63 -0.8 3,167,127 - NIAGMN_16000 0.67 +0.7 3,167,134 + NIAGMN_16000 0.68 +0.0 3,167,134 + NIAGMN_16000 0.68 +1.0 3,167,135 - NIAGMN_16000 0.68 -0.4 3,167,164 + NIAGMN_16000 0.74 -0.6 3,167,195 - NIAGMN_16000 0.80 -0.5 3,167,200 + NIAGMN_16000 0.82 -1.8 3,167,200 + NIAGMN_16000 0.82 -1.0 3,167,201 - NIAGMN_16000 0.82 -0.2 3,167,201 - NIAGMN_16000 0.82 +1.1 3,167,203 + NIAGMN_16000 0.82 +0.7 3,167,203 + NIAGMN_16000 0.82 -0.6 3,167,204 - NIAGMN_16000 0.82 -1.1 3,167,204 - NIAGMN_16000 0.82 +0.8 3,167,204 - NIAGMN_16000 0.82 -0.3 3,167,204 - NIAGMN_16000 0.82 +0.6 3,167,246 - -0.8 3,167,246 - -0.3 3,167,246 - -2.0
Or see this region's nucleotide sequence