Strain Fitness in Escherichia coli ECRC102 around NIAGMN_07575

Experiment: Sodium-DL-Lactate

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntprfH and ykfJ overlap by 32 nucleotidesykfJ and yafP are separated by 306 nucleotidesyafP and yafO are separated by 84 nucleotidesyafO and yafN are separated by 2 nucleotidesyafN and dinB are separated by 51 nucleotides NIAGMN_07565: prfH - peptide chain release factor H, at 1,449,955 to 1,450,455 prfH NIAGMN_07570: ykfJ - Putative uncharacterized protein YkfJ, at 1,450,424 to 1,450,690 ykfJ NIAGMN_07575: yafP - GNAT family N-acetyltransferase, at 1,450,997 to 1,451,374 yafP NIAGMN_07580: yafO - type II toxin-antitoxin system mRNA interferase toxin YafO, at 1,451,459 to 1,451,857 yafO NIAGMN_07585: yafN - type I toxin-antitoxin system antitoxin YafN, at 1,451,860 to 1,452,153 yafN NIAGMN_07590: dinB - DNA polymerase IV, at 1,452,205 to 1,453,260 dinB Position (kb) 1450 1451 1452Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 1450.054 kb on - strand, within prfHat 1450.054 kb on - strand, within prfHat 1450.113 kb on + strand, within prfHat 1450.114 kb on - strand, within prfHat 1450.114 kb on - strand, within prfHat 1450.178 kb on - strand, within prfHat 1450.372 kb on + strand, within prfHat 1450.479 kb on + strand, within ykfJat 1450.535 kb on + strand, within ykfJat 1450.536 kb on - strand, within ykfJat 1450.640 kb on - strand, within ykfJat 1450.640 kb on - strand, within ykfJat 1450.643 kb on + strand, within ykfJat 1450.667 kb on - strandat 1450.669 kb on - strandat 1450.669 kb on - strandat 1450.681 kb on - strandat 1450.704 kb on - strandat 1450.720 kb on - strandat 1450.720 kb on - strandat 1450.752 kb on - strandat 1450.752 kb on - strandat 1450.752 kb on - strandat 1450.756 kb on - strandat 1450.756 kb on - strandat 1450.756 kb on - strandat 1450.756 kb on - strandat 1450.760 kb on - strandat 1450.760 kb on - strandat 1450.815 kb on + strandat 1450.817 kb on + strandat 1450.817 kb on + strandat 1450.819 kb on + strandat 1450.824 kb on - strandat 1451.213 kb on - strand, within yafPat 1451.393 kb on - strandat 1451.399 kb on + strandat 1451.400 kb on - strandat 1451.425 kb on - strandat 1451.439 kb on - strandat 1451.595 kb on + strand, within yafOat 1451.596 kb on - strand, within yafOat 1451.677 kb on + strand, within yafOat 1451.677 kb on + strand, within yafOat 1451.747 kb on + strand, within yafOat 1451.748 kb on - strand, within yafOat 1451.750 kb on - strand, within yafOat 1451.776 kb on - strand, within yafOat 1451.819 kb on - strandat 1452.153 kb on + strandat 1452.169 kb on + strandat 1452.190 kb on - strandat 1452.209 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Sodium-DL-Lactate
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1,450,054 - prfH NIAGMN_07565 0.20 +0.1
1,450,054 - prfH NIAGMN_07565 0.20 +0.6
1,450,113 + prfH NIAGMN_07565 0.32 -0.4
1,450,114 - prfH NIAGMN_07565 0.32 +0.7
1,450,114 - prfH NIAGMN_07565 0.32 -0.5
1,450,178 - prfH NIAGMN_07565 0.45 +0.3
1,450,372 + prfH NIAGMN_07565 0.83 -1.2
1,450,479 + ykfJ NIAGMN_07570 0.21 -0.1
1,450,535 + ykfJ NIAGMN_07570 0.42 -1.6
1,450,536 - ykfJ NIAGMN_07570 0.42 -0.5
1,450,640 - ykfJ NIAGMN_07570 0.81 -0.6
1,450,640 - ykfJ NIAGMN_07570 0.81 -1.1
1,450,643 + ykfJ NIAGMN_07570 0.82 -0.6
1,450,667 - +0.7
1,450,669 - -1.8
1,450,669 - +0.6
1,450,681 - +0.0
1,450,704 - -0.1
1,450,720 - +1.6
1,450,720 - +1.3
1,450,752 - -0.1
1,450,752 - -0.4
1,450,752 - -1.8
1,450,756 - +0.0
1,450,756 - -1.8
1,450,756 - -0.9
1,450,756 - -0.0
1,450,760 - +0.7
1,450,760 - +0.7
1,450,815 + +1.0
1,450,817 + -2.8
1,450,817 + -2.2
1,450,819 + +2.2
1,450,824 - +1.8
1,451,213 - yafP NIAGMN_07575 0.57 -0.2
1,451,393 - +0.1
1,451,399 + +0.5
1,451,400 - +1.3
1,451,425 - +0.2
1,451,439 - +2.1
1,451,595 + yafO NIAGMN_07580 0.34 +0.1
1,451,596 - yafO NIAGMN_07580 0.34 -0.9
1,451,677 + yafO NIAGMN_07580 0.55 +0.5
1,451,677 + yafO NIAGMN_07580 0.55 +2.1
1,451,747 + yafO NIAGMN_07580 0.72 +0.1
1,451,748 - yafO NIAGMN_07580 0.72 -2.2
1,451,750 - yafO NIAGMN_07580 0.73 -2.0
1,451,776 - yafO NIAGMN_07580 0.79 -0.3
1,451,819 - +0.5
1,452,153 + -2.4
1,452,169 + +0.1
1,452,190 - +0.1
1,452,209 - -2.1

Or see this region's nucleotide sequence