Experiment: Sodium-DL-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nagD and asnB are separated by 256 nucleotides asnB and Met_trna are separated by 379 nucleotides Met_trna and Leu_trna are separated by 10 nucleotides Leu_trna and Gln_trna are separated by 23 nucleotides Gln_trna and Gln_trna are separated by 34 nucleotides Gln_trna and Met_trna are separated by 15 nucleotides Met_trna and Gln_trna are separated by 48 nucleotides
NIAGMN_05230: nagD - ribonucleotide monophosphatase NagD, at 960,691 to 961,443
nagD
NIAGMN_05235: asnB - asparagine synthase B, at 961,700 to 963,364
asnB
NIAGMN_05240: Met_trna - tRNA-Met(cat), at 963,744 to 963,820
_trna
NIAGMN_05245: Leu_trna - tRNA-Leu(tag), at 963,831 to 963,915
_trna
NIAGMN_05250: Gln_trna - tRNA-Gln(ttg), at 963,939 to 964,013
_trna
NIAGMN_05255: Gln_trna - tRNA-Gln(ttg), at 964,048 to 964,122
_trna
NIAGMN_05260: Met_trna - tRNA-Met(cat), at 964,138 to 964,214
_trna
NIAGMN_05265: Gln_trna - tRNA-Gln(ctg), at 964,263 to 964,337
_trna
Position (kb) 961
962
963
964 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 960.820 kb on + strand, within nagD at 960.878 kb on + strand, within nagD at 960.896 kb on - strand, within nagD at 960.896 kb on - strand, within nagD at 960.897 kb on + strand, within nagD at 960.898 kb on - strand, within nagD at 960.952 kb on + strand, within nagD at 960.952 kb on + strand, within nagD at 960.952 kb on + strand, within nagD at 960.953 kb on - strand, within nagD at 960.953 kb on - strand, within nagD at 960.988 kb on + strand, within nagD at 960.988 kb on + strand, within nagD at 960.988 kb on + strand, within nagD at 960.989 kb on - strand, within nagD at 960.989 kb on - strand, within nagD at 960.989 kb on - strand, within nagD at 960.989 kb on - strand, within nagD at 961.054 kb on + strand, within nagD at 961.054 kb on + strand, within nagD at 961.054 kb on + strand, within nagD at 961.054 kb on + strand, within nagD at 961.055 kb on - strand, within nagD at 961.055 kb on - strand, within nagD at 961.074 kb on + strand, within nagD at 961.085 kb on - strand, within nagD at 961.111 kb on - strand, within nagD at 961.207 kb on + strand, within nagD at 961.207 kb on + strand, within nagD at 961.207 kb on + strand, within nagD at 961.208 kb on - strand, within nagD at 961.208 kb on - strand, within nagD at 961.208 kb on - strand, within nagD at 961.208 kb on - strand, within nagD at 961.208 kb on - strand, within nagD at 961.215 kb on + strand, within nagD at 961.215 kb on + strand, within nagD at 961.247 kb on + strand, within nagD at 961.308 kb on + strand, within nagD at 961.386 kb on + strand at 961.386 kb on + strand at 961.386 kb on + strand at 961.411 kb on + strand at 961.437 kb on + strand at 961.437 kb on + strand at 961.445 kb on + strand at 961.491 kb on - strand at 961.589 kb on - strand at 961.589 kb on - strand at 961.678 kb on + strand at 961.678 kb on + strand at 961.678 kb on + strand at 961.679 kb on - strand at 961.699 kb on + strand at 961.699 kb on + strand at 961.700 kb on - strand at 961.700 kb on - strand at 961.700 kb on - strand at 961.700 kb on - strand at 961.700 kb on - strand at 961.815 kb on - strand at 961.890 kb on - strand, within asnB at 961.912 kb on - strand, within asnB at 961.934 kb on + strand, within asnB at 961.973 kb on + strand, within asnB at 962.099 kb on + strand, within asnB at 962.099 kb on + strand, within asnB at 962.138 kb on + strand, within asnB at 962.138 kb on + strand, within asnB at 962.138 kb on + strand, within asnB at 962.138 kb on + strand, within asnB at 962.139 kb on - strand, within asnB at 962.139 kb on - strand, within asnB at 962.139 kb on - strand, within asnB at 962.139 kb on - strand, within asnB at 962.139 kb on - strand, within asnB at 962.139 kb on - strand, within asnB at 962.139 kb on - strand, within asnB at 962.139 kb on - strand, within asnB at 962.139 kb on - strand, within asnB at 962.147 kb on + strand, within asnB at 962.147 kb on + strand, within asnB at 962.148 kb on - strand, within asnB at 962.168 kb on + strand, within asnB at 962.168 kb on + strand, within asnB at 962.169 kb on - strand, within asnB at 962.169 kb on - strand, within asnB at 962.169 kb on - strand, within asnB at 962.169 kb on - strand, within asnB at 962.237 kb on + strand, within asnB at 962.237 kb on + strand, within asnB at 962.237 kb on + strand, within asnB at 962.238 kb on - strand, within asnB at 962.238 kb on - strand, within asnB at 962.238 kb on - strand, within asnB at 962.238 kb on - strand, within asnB at 962.238 kb on - strand, within asnB at 962.270 kb on + strand, within asnB at 962.270 kb on + strand, within asnB at 962.270 kb on + strand, within asnB at 962.270 kb on + strand, within asnB at 962.271 kb on - strand, within asnB at 962.426 kb on - strand, within asnB at 962.435 kb on - strand, within asnB at 962.447 kb on + strand, within asnB at 962.503 kb on - strand, within asnB at 962.517 kb on + strand, within asnB at 962.517 kb on + strand, within asnB at 962.605 kb on - strand, within asnB at 962.640 kb on - strand, within asnB at 962.655 kb on - strand, within asnB at 962.690 kb on + strand, within asnB at 962.691 kb on - strand, within asnB at 962.691 kb on - strand, within asnB at 962.691 kb on - strand, within asnB at 962.691 kb on - strand, within asnB at 962.691 kb on - strand, within asnB at 962.694 kb on + strand, within asnB at 962.726 kb on - strand, within asnB at 962.777 kb on + strand, within asnB at 962.777 kb on + strand, within asnB at 962.845 kb on + strand, within asnB at 962.846 kb on - strand, within asnB at 962.846 kb on - strand, within asnB at 962.849 kb on + strand, within asnB at 962.849 kb on + strand, within asnB at 962.849 kb on + strand, within asnB at 962.849 kb on + strand, within asnB at 962.849 kb on + strand, within asnB at 962.849 kb on + strand, within asnB at 962.849 kb on + strand, within asnB at 962.849 kb on + strand, within asnB at 962.850 kb on - strand, within asnB at 962.850 kb on - strand, within asnB at 962.850 kb on - strand, within asnB at 963.017 kb on + strand, within asnB at 963.017 kb on + strand, within asnB at 963.017 kb on + strand, within asnB at 963.018 kb on - strand, within asnB at 963.018 kb on - strand, within asnB at 963.158 kb on + strand, within asnB at 963.158 kb on + strand, within asnB at 963.158 kb on + strand, within asnB at 963.159 kb on - strand, within asnB at 963.185 kb on + strand, within asnB at 963.185 kb on + strand, within asnB at 963.185 kb on + strand, within asnB at 963.186 kb on - strand, within asnB at 963.186 kb on - strand, within asnB at 963.213 kb on + strand at 963.213 kb on + strand at 963.213 kb on + strand at 963.214 kb on - strand at 963.356 kb on + strand at 963.358 kb on + strand at 963.358 kb on + strand at 963.358 kb on + strand at 963.358 kb on + strand at 963.362 kb on + strand at 963.362 kb on + strand at 963.362 kb on + strand at 963.363 kb on - strand at 963.407 kb on + strand at 963.438 kb on + strand at 963.489 kb on - strand at 963.489 kb on - strand at 963.489 kb on - strand at 963.489 kb on - strand at 963.509 kb on - strand at 963.528 kb on + strand at 963.549 kb on + strand at 963.550 kb on - strand at 963.569 kb on - strand at 963.569 kb on - strand at 963.591 kb on - strand at 963.598 kb on - strand at 963.598 kb on - strand at 963.598 kb on - strand at 963.598 kb on - strand at 963.632 kb on + strand at 964.206 kb on + strand, within Met_trna at 964.354 kb on + strand at 964.354 kb on + strand at 964.354 kb on + strand at 964.354 kb on + strand at 964.354 kb on + strand at 964.361 kb on + strand at 964.361 kb on + strand at 964.361 kb on + strand at 964.361 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Sodium-DL-Lactate remove 960,820 + nagD NIAGMN_05230 0.17 -0.0 960,878 + nagD NIAGMN_05230 0.25 +0.3 960,896 - nagD NIAGMN_05230 0.27 +0.9 960,896 - nagD NIAGMN_05230 0.27 -0.6 960,897 + nagD NIAGMN_05230 0.27 +0.4 960,898 - nagD NIAGMN_05230 0.27 -0.6 960,952 + nagD NIAGMN_05230 0.35 +2.5 960,952 + nagD NIAGMN_05230 0.35 -1.0 960,952 + nagD NIAGMN_05230 0.35 -0.5 960,953 - nagD NIAGMN_05230 0.35 -0.1 960,953 - nagD NIAGMN_05230 0.35 -1.3 960,988 + nagD NIAGMN_05230 0.39 -1.2 960,988 + nagD NIAGMN_05230 0.39 -0.2 960,988 + nagD NIAGMN_05230 0.39 +0.4 960,989 - nagD NIAGMN_05230 0.40 +0.5 960,989 - nagD NIAGMN_05230 0.40 -0.4 960,989 - nagD NIAGMN_05230 0.40 +0.7 960,989 - nagD NIAGMN_05230 0.40 +0.2 961,054 + nagD NIAGMN_05230 0.48 -0.8 961,054 + nagD NIAGMN_05230 0.48 +0.4 961,054 + nagD NIAGMN_05230 0.48 +0.1 961,054 + nagD NIAGMN_05230 0.48 -0.3 961,055 - nagD NIAGMN_05230 0.48 -0.7 961,055 - nagD NIAGMN_05230 0.48 +0.3 961,074 + nagD NIAGMN_05230 0.51 -1.7 961,085 - nagD NIAGMN_05230 0.52 -0.7 961,111 - nagD NIAGMN_05230 0.56 -0.5 961,207 + nagD NIAGMN_05230 0.69 -1.0 961,207 + nagD NIAGMN_05230 0.69 +0.5 961,207 + nagD NIAGMN_05230 0.69 +0.6 961,208 - nagD NIAGMN_05230 0.69 -1.4 961,208 - nagD NIAGMN_05230 0.69 +0.2 961,208 - nagD NIAGMN_05230 0.69 +0.6 961,208 - nagD NIAGMN_05230 0.69 -0.4 961,208 - nagD NIAGMN_05230 0.69 +0.0 961,215 + nagD NIAGMN_05230 0.70 -0.0 961,215 + nagD NIAGMN_05230 0.70 +2.5 961,247 + nagD NIAGMN_05230 0.74 -0.4 961,308 + nagD NIAGMN_05230 0.82 -1.5 961,386 + +1.8 961,386 + +0.4 961,386 + -1.4 961,411 + -1.0 961,437 + -0.9 961,437 + +0.4 961,445 + -1.0 961,491 - -0.9 961,589 - +1.1 961,589 - -0.6 961,678 + -0.4 961,678 + -1.3 961,678 + -0.9 961,679 - +0.3 961,699 + +0.2 961,699 + +0.5 961,700 - +0.6 961,700 - +1.3 961,700 - -2.1 961,700 - +1.5 961,700 - -1.4 961,815 - -0.3 961,890 - asnB NIAGMN_05235 0.11 -0.0 961,912 - asnB NIAGMN_05235 0.13 -0.7 961,934 + asnB NIAGMN_05235 0.14 -2.1 961,973 + asnB NIAGMN_05235 0.16 +0.4 962,099 + asnB NIAGMN_05235 0.24 +2.3 962,099 + asnB NIAGMN_05235 0.24 -0.8 962,138 + asnB NIAGMN_05235 0.26 +0.5 962,138 + asnB NIAGMN_05235 0.26 -1.0 962,138 + asnB NIAGMN_05235 0.26 -0.0 962,138 + asnB NIAGMN_05235 0.26 -0.6 962,139 - asnB NIAGMN_05235 0.26 -0.8 962,139 - asnB NIAGMN_05235 0.26 +1.4 962,139 - asnB NIAGMN_05235 0.26 +1.7 962,139 - asnB NIAGMN_05235 0.26 +0.2 962,139 - asnB NIAGMN_05235 0.26 +2.4 962,139 - asnB NIAGMN_05235 0.26 +1.3 962,139 - asnB NIAGMN_05235 0.26 -1.0 962,139 - asnB NIAGMN_05235 0.26 +1.4 962,139 - asnB NIAGMN_05235 0.26 -1.2 962,147 + asnB NIAGMN_05235 0.27 -0.9 962,147 + asnB NIAGMN_05235 0.27 -0.2 962,148 - asnB NIAGMN_05235 0.27 +0.8 962,168 + asnB NIAGMN_05235 0.28 +0.5 962,168 + asnB NIAGMN_05235 0.28 +0.2 962,169 - asnB NIAGMN_05235 0.28 +0.4 962,169 - asnB NIAGMN_05235 0.28 -0.7 962,169 - asnB NIAGMN_05235 0.28 -3.2 962,169 - asnB NIAGMN_05235 0.28 +0.2 962,237 + asnB NIAGMN_05235 0.32 -1.3 962,237 + asnB NIAGMN_05235 0.32 +1.0 962,237 + asnB NIAGMN_05235 0.32 -0.9 962,238 - asnB NIAGMN_05235 0.32 +0.2 962,238 - asnB NIAGMN_05235 0.32 -3.1 962,238 - asnB NIAGMN_05235 0.32 +2.5 962,238 - asnB NIAGMN_05235 0.32 -0.0 962,238 - asnB NIAGMN_05235 0.32 +1.5 962,270 + asnB NIAGMN_05235 0.34 +2.9 962,270 + asnB NIAGMN_05235 0.34 -0.3 962,270 + asnB NIAGMN_05235 0.34 -0.2 962,270 + asnB NIAGMN_05235 0.34 +0.1 962,271 - asnB NIAGMN_05235 0.34 -0.2 962,426 - asnB NIAGMN_05235 0.44 +1.7 962,435 - asnB NIAGMN_05235 0.44 -2.3 962,447 + asnB NIAGMN_05235 0.45 +2.8 962,503 - asnB NIAGMN_05235 0.48 -1.2 962,517 + asnB NIAGMN_05235 0.49 +0.9 962,517 + asnB NIAGMN_05235 0.49 +0.4 962,605 - asnB NIAGMN_05235 0.54 -0.3 962,640 - asnB NIAGMN_05235 0.56 +0.2 962,655 - asnB NIAGMN_05235 0.57 -0.8 962,690 + asnB NIAGMN_05235 0.59 +0.1 962,691 - asnB NIAGMN_05235 0.60 -0.2 962,691 - asnB NIAGMN_05235 0.60 +0.7 962,691 - asnB NIAGMN_05235 0.60 +1.1 962,691 - asnB NIAGMN_05235 0.60 -0.9 962,691 - asnB NIAGMN_05235 0.60 +0.1 962,694 + asnB NIAGMN_05235 0.60 +0.9 962,726 - asnB NIAGMN_05235 0.62 +1.6 962,777 + asnB NIAGMN_05235 0.65 -0.9 962,777 + asnB NIAGMN_05235 0.65 +0.4 962,845 + asnB NIAGMN_05235 0.69 -0.1 962,846 - asnB NIAGMN_05235 0.69 -0.0 962,846 - asnB NIAGMN_05235 0.69 -2.0 962,849 + asnB NIAGMN_05235 0.69 -2.9 962,849 + asnB NIAGMN_05235 0.69 +0.4 962,849 + asnB NIAGMN_05235 0.69 -1.6 962,849 + asnB NIAGMN_05235 0.69 -0.5 962,849 + asnB NIAGMN_05235 0.69 -1.4 962,849 + asnB NIAGMN_05235 0.69 -0.4 962,849 + asnB NIAGMN_05235 0.69 -1.0 962,849 + asnB NIAGMN_05235 0.69 -1.6 962,850 - asnB NIAGMN_05235 0.69 +1.1 962,850 - asnB NIAGMN_05235 0.69 -1.0 962,850 - asnB NIAGMN_05235 0.69 +1.1 963,017 + asnB NIAGMN_05235 0.79 -1.1 963,017 + asnB NIAGMN_05235 0.79 -1.8 963,017 + asnB NIAGMN_05235 0.79 -0.3 963,018 - asnB NIAGMN_05235 0.79 +0.5 963,018 - asnB NIAGMN_05235 0.79 +0.5 963,158 + asnB NIAGMN_05235 0.88 +1.2 963,158 + asnB NIAGMN_05235 0.88 -0.0 963,158 + asnB NIAGMN_05235 0.88 +0.3 963,159 - asnB NIAGMN_05235 0.88 -0.1 963,185 + asnB NIAGMN_05235 0.89 -0.3 963,185 + asnB NIAGMN_05235 0.89 +0.5 963,185 + asnB NIAGMN_05235 0.89 -1.6 963,186 - asnB NIAGMN_05235 0.89 +0.5 963,186 - asnB NIAGMN_05235 0.89 +0.0 963,213 + +0.2 963,213 + -2.5 963,213 + +0.4 963,214 - -0.9 963,356 + +0.2 963,358 + +0.5 963,358 + -0.1 963,358 + +0.0 963,358 + +1.2 963,362 + -0.4 963,362 + -1.5 963,362 + -0.3 963,363 - +0.8 963,407 + -0.8 963,438 + -0.4 963,489 - +0.6 963,489 - -0.6 963,489 - +1.2 963,489 - -0.8 963,509 - -0.1 963,528 + +0.8 963,549 + -0.6 963,550 - +0.5 963,569 - -0.6 963,569 - +0.7 963,591 - +1.6 963,598 - -0.2 963,598 - -0.7 963,598 - +0.4 963,598 - +0.0 963,632 + -0.9 964,206 + Met_trna NIAGMN_05260 0.88 -0.4 964,354 + -0.0 964,354 + -0.1 964,354 + +2.5 964,354 + -3.1 964,354 + -1.9 964,361 + +0.1 964,361 + +0.2 964,361 + -0.7 964,361 + -0.8
Or see this region's nucleotide sequence