Experiment: Sodium-DL-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nagE and nagB are separated by 332 nucleotides nagB and nagA are separated by 59 nucleotides
NIAGMN_05210: nagE - PTS N-acetyl glucosamine transporter subunit IIABC, at 955,127 to 957,073
nagE
NIAGMN_05215: nagB - glucosamine-6-phosphate deaminase, at 957,406 to 958,206
nagB
NIAGMN_05220: nagA - N-acetylglucosamine-6-phosphate deacetylase, at 958,266 to 959,414
nagA
Position (kb) 957
958
959 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 956.599 kb on + strand, within nagE at 956.625 kb on + strand, within nagE at 956.719 kb on + strand, within nagE at 956.739 kb on + strand, within nagE at 956.740 kb on - strand, within nagE at 956.740 kb on - strand, within nagE at 956.740 kb on - strand, within nagE at 956.835 kb on + strand, within nagE at 956.836 kb on - strand, within nagE at 956.836 kb on - strand, within nagE at 956.924 kb on - strand at 956.965 kb on + strand at 956.965 kb on + strand at 956.966 kb on - strand at 956.966 kb on - strand at 956.969 kb on + strand at 957.068 kb on - strand at 957.076 kb on + strand at 957.076 kb on + strand at 957.076 kb on + strand at 957.077 kb on - strand at 957.077 kb on - strand at 957.077 kb on - strand at 957.096 kb on + strand at 957.097 kb on - strand at 957.097 kb on - strand at 957.168 kb on + strand at 957.169 kb on - strand at 957.169 kb on - strand at 957.169 kb on - strand at 957.272 kb on + strand at 957.272 kb on + strand at 957.293 kb on + strand at 957.365 kb on + strand at 957.382 kb on - strand at 957.392 kb on + strand at 957.393 kb on - strand at 957.426 kb on + strand at 957.550 kb on + strand, within nagB at 957.551 kb on - strand, within nagB at 957.574 kb on - strand, within nagB at 957.658 kb on + strand, within nagB at 957.659 kb on - strand, within nagB at 957.659 kb on - strand, within nagB at 957.822 kb on + strand, within nagB at 957.834 kb on + strand, within nagB at 957.834 kb on + strand, within nagB at 957.873 kb on + strand, within nagB at 957.955 kb on + strand, within nagB at 957.978 kb on + strand, within nagB at 957.978 kb on + strand, within nagB at 958.071 kb on + strand, within nagB at 958.071 kb on + strand, within nagB at 958.071 kb on + strand, within nagB at 958.072 kb on - strand, within nagB at 958.204 kb on + strand at 958.269 kb on + strand at 958.269 kb on + strand at 958.269 kb on + strand at 958.541 kb on + strand, within nagA at 958.544 kb on + strand, within nagA at 958.544 kb on + strand, within nagA at 958.544 kb on + strand, within nagA at 958.548 kb on + strand, within nagA at 958.548 kb on + strand, within nagA at 958.562 kb on + strand, within nagA at 958.562 kb on + strand, within nagA at 958.712 kb on + strand, within nagA at 958.898 kb on + strand, within nagA at 958.917 kb on + strand, within nagA at 958.917 kb on + strand, within nagA at 958.932 kb on + strand, within nagA at 958.932 kb on + strand, within nagA at 958.932 kb on + strand, within nagA at 958.932 kb on + strand, within nagA at 958.934 kb on + strand, within nagA at 958.989 kb on + strand, within nagA at 958.989 kb on + strand, within nagA at 958.989 kb on + strand, within nagA at 959.000 kb on + strand, within nagA at 959.045 kb on + strand, within nagA at 959.046 kb on - strand, within nagA at 959.142 kb on + strand, within nagA at 959.142 kb on + strand, within nagA at 959.142 kb on + strand, within nagA at 959.142 kb on + strand, within nagA at 959.142 kb on + strand, within nagA at 959.142 kb on + strand, within nagA at 959.142 kb on + strand, within nagA at 959.142 kb on + strand, within nagA at 959.145 kb on + strand, within nagA at 959.145 kb on + strand, within nagA
Per-strain Table
Position Strand Gene LocusTag Fraction Sodium-DL-Lactate remove 956,599 + nagE NIAGMN_05210 0.76 -2.0 956,625 + nagE NIAGMN_05210 0.77 -3.1 956,719 + nagE NIAGMN_05210 0.82 -2.4 956,739 + nagE NIAGMN_05210 0.83 +0.4 956,740 - nagE NIAGMN_05210 0.83 +0.5 956,740 - nagE NIAGMN_05210 0.83 -0.3 956,740 - nagE NIAGMN_05210 0.83 -0.2 956,835 + nagE NIAGMN_05210 0.88 +0.2 956,836 - nagE NIAGMN_05210 0.88 -0.8 956,836 - nagE NIAGMN_05210 0.88 -0.8 956,924 - +0.6 956,965 + +0.2 956,965 + -1.0 956,966 - +0.8 956,966 - +1.0 956,969 + +1.5 957,068 - -1.1 957,076 + -1.0 957,076 + +1.4 957,076 + -1.0 957,077 - +0.7 957,077 - +0.3 957,077 - -1.4 957,096 + +0.4 957,097 - +0.4 957,097 - -0.4 957,168 + -3.7 957,169 - +0.0 957,169 - -1.2 957,169 - +0.9 957,272 + -0.4 957,272 + -0.5 957,293 + -0.5 957,365 + -0.6 957,382 - +1.4 957,392 + +0.2 957,393 - +0.1 957,426 + +0.1 957,550 + nagB NIAGMN_05215 0.18 -1.1 957,551 - nagB NIAGMN_05215 0.18 +1.8 957,574 - nagB NIAGMN_05215 0.21 -0.0 957,658 + nagB NIAGMN_05215 0.31 -0.5 957,659 - nagB NIAGMN_05215 0.32 -0.9 957,659 - nagB NIAGMN_05215 0.32 +1.4 957,822 + nagB NIAGMN_05215 0.52 +2.4 957,834 + nagB NIAGMN_05215 0.53 +1.4 957,834 + nagB NIAGMN_05215 0.53 +1.7 957,873 + nagB NIAGMN_05215 0.58 -0.3 957,955 + nagB NIAGMN_05215 0.69 +0.4 957,978 + nagB NIAGMN_05215 0.71 -0.8 957,978 + nagB NIAGMN_05215 0.71 +0.3 958,071 + nagB NIAGMN_05215 0.83 +0.4 958,071 + nagB NIAGMN_05215 0.83 -0.4 958,071 + nagB NIAGMN_05215 0.83 -0.1 958,072 - nagB NIAGMN_05215 0.83 -1.3 958,204 + -0.7 958,269 + +0.4 958,269 + -1.6 958,269 + +3.5 958,541 + nagA NIAGMN_05220 0.24 -0.8 958,544 + nagA NIAGMN_05220 0.24 -0.7 958,544 + nagA NIAGMN_05220 0.24 -1.4 958,544 + nagA NIAGMN_05220 0.24 -1.7 958,548 + nagA NIAGMN_05220 0.25 -0.1 958,548 + nagA NIAGMN_05220 0.25 +0.3 958,562 + nagA NIAGMN_05220 0.26 -0.8 958,562 + nagA NIAGMN_05220 0.26 +0.2 958,712 + nagA NIAGMN_05220 0.39 -1.6 958,898 + nagA NIAGMN_05220 0.55 -1.3 958,917 + nagA NIAGMN_05220 0.57 +0.4 958,917 + nagA NIAGMN_05220 0.57 -0.8 958,932 + nagA NIAGMN_05220 0.58 -1.4 958,932 + nagA NIAGMN_05220 0.58 -0.9 958,932 + nagA NIAGMN_05220 0.58 -1.8 958,932 + nagA NIAGMN_05220 0.58 -1.2 958,934 + nagA NIAGMN_05220 0.58 +1.8 958,989 + nagA NIAGMN_05220 0.63 -0.7 958,989 + nagA NIAGMN_05220 0.63 +0.8 958,989 + nagA NIAGMN_05220 0.63 -1.4 959,000 + nagA NIAGMN_05220 0.64 -1.8 959,045 + nagA NIAGMN_05220 0.68 -0.1 959,046 - nagA NIAGMN_05220 0.68 +0.9 959,142 + nagA NIAGMN_05220 0.76 -2.0 959,142 + nagA NIAGMN_05220 0.76 +0.4 959,142 + nagA NIAGMN_05220 0.76 -2.2 959,142 + nagA NIAGMN_05220 0.76 -2.2 959,142 + nagA NIAGMN_05220 0.76 -0.7 959,142 + nagA NIAGMN_05220 0.76 -0.9 959,142 + nagA NIAGMN_05220 0.76 -2.9 959,142 + nagA NIAGMN_05220 0.76 -1.0 959,145 + nagA NIAGMN_05220 0.77 +0.0 959,145 + nagA NIAGMN_05220 0.77 -1.2
Or see this region's nucleotide sequence