Experiment: Sodium-DL-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ybiI and ybiJ are separated by 264 nucleotides ybiJ and ybiE are separated by 102 nucleotides ybiE and hcxB are separated by 66 nucleotides hcxB and ybiB are separated by 140 nucleotides
NIAGMN_04295: ybiI - C4-type zinc finger protein YbiI, at 778,144 to 778,410
ybiI
NIAGMN_04300: ybiJ - DUF1471 domain-containing protein, at 778,675 to 778,935
ybiJ
NIAGMN_04310: ybiE - Protein YbiE, at 779,038 to 779,097
ybiE
NIAGMN_04315: hcxB - hydroxycarboxylate dehydrogenase HcXB, at 779,164 to 780,249
hcxB
NIAGMN_04320: ybiB - DNA-binding protein YbiB, at 780,390 to 781,352
ybiB
Position (kb) 779
780
781 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 778.320 kb on - strand, within ybiI at 778.323 kb on + strand, within ybiI at 778.323 kb on + strand, within ybiI at 778.323 kb on + strand, within ybiI at 778.323 kb on + strand, within ybiI at 778.363 kb on + strand, within ybiI at 778.363 kb on + strand, within ybiI at 778.366 kb on - strand, within ybiI at 778.408 kb on + strand at 778.412 kb on + strand at 778.412 kb on + strand at 778.412 kb on + strand at 778.428 kb on + strand at 778.428 kb on + strand at 778.539 kb on + strand at 778.645 kb on + strand at 778.646 kb on - strand at 778.646 kb on - strand at 778.647 kb on + strand at 778.647 kb on + strand at 778.670 kb on + strand at 778.670 kb on + strand at 778.670 kb on + strand at 778.683 kb on + strand at 778.689 kb on + strand at 778.875 kb on + strand, within ybiJ at 778.875 kb on + strand, within ybiJ at 778.875 kb on + strand, within ybiJ at 778.876 kb on - strand, within ybiJ at 778.902 kb on + strand, within ybiJ at 778.902 kb on + strand, within ybiJ at 778.927 kb on + strand at 778.927 kb on + strand at 778.927 kb on + strand at 778.933 kb on + strand at 778.933 kb on + strand at 778.933 kb on + strand at 778.933 kb on + strand at 779.005 kb on - strand at 779.005 kb on - strand at 779.013 kb on + strand at 779.047 kb on + strand, within ybiE at 779.047 kb on + strand, within ybiE at 779.098 kb on - strand at 779.120 kb on + strand at 779.120 kb on + strand at 779.120 kb on + strand at 779.121 kb on - strand at 779.121 kb on - strand at 779.125 kb on + strand at 779.125 kb on + strand at 779.389 kb on - strand, within hcxB at 779.389 kb on - strand, within hcxB at 779.443 kb on - strand, within hcxB at 779.468 kb on - strand, within hcxB at 779.662 kb on - strand, within hcxB at 779.737 kb on - strand, within hcxB at 779.881 kb on - strand, within hcxB at 779.922 kb on + strand, within hcxB at 780.079 kb on + strand, within hcxB at 780.080 kb on - strand, within hcxB at 780.080 kb on - strand, within hcxB at 780.083 kb on + strand, within hcxB at 780.084 kb on - strand, within hcxB at 780.205 kb on - strand at 780.254 kb on - strand at 780.264 kb on + strand at 780.264 kb on + strand at 780.265 kb on - strand at 780.269 kb on + strand at 780.270 kb on - strand at 780.429 kb on - strand at 780.429 kb on - strand at 780.651 kb on - strand, within ybiB at 780.656 kb on - strand, within ybiB at 780.734 kb on - strand, within ybiB at 780.734 kb on - strand, within ybiB at 780.806 kb on + strand, within ybiB at 780.819 kb on - strand, within ybiB at 780.887 kb on + strand, within ybiB at 780.888 kb on - strand, within ybiB at 780.888 kb on - strand, within ybiB
Per-strain Table
Position Strand Gene LocusTag Fraction Sodium-DL-Lactate remove 778,320 - ybiI NIAGMN_04295 0.66 +0.9 778,323 + ybiI NIAGMN_04295 0.67 -2.4 778,323 + ybiI NIAGMN_04295 0.67 +0.2 778,323 + ybiI NIAGMN_04295 0.67 -0.6 778,323 + ybiI NIAGMN_04295 0.67 -0.3 778,363 + ybiI NIAGMN_04295 0.82 -0.7 778,363 + ybiI NIAGMN_04295 0.82 +3.2 778,366 - ybiI NIAGMN_04295 0.83 +1.9 778,408 + +0.7 778,412 + +2.5 778,412 + +1.4 778,412 + +1.5 778,428 + +0.7 778,428 + +2.3 778,539 + -1.4 778,645 + +0.2 778,646 - +1.1 778,646 - -1.8 778,647 + +0.4 778,647 + +1.9 778,670 + -0.1 778,670 + +2.6 778,670 + +0.0 778,683 + -0.5 778,689 + +0.6 778,875 + ybiJ NIAGMN_04300 0.77 +0.7 778,875 + ybiJ NIAGMN_04300 0.77 +2.1 778,875 + ybiJ NIAGMN_04300 0.77 -2.4 778,876 - ybiJ NIAGMN_04300 0.77 +0.1 778,902 + ybiJ NIAGMN_04300 0.87 +0.0 778,902 + ybiJ NIAGMN_04300 0.87 -0.1 778,927 + -0.3 778,927 + -2.2 778,927 + +1.4 778,933 + -1.5 778,933 + +2.5 778,933 + +0.0 778,933 + -0.8 779,005 - +0.2 779,005 - +0.5 779,013 + -0.3 779,047 + ybiE NIAGMN_04310 0.15 +0.1 779,047 + ybiE NIAGMN_04310 0.15 +1.7 779,098 - -1.4 779,120 + -1.4 779,120 + +0.0 779,120 + -2.9 779,121 - -0.9 779,121 - +0.4 779,125 + -1.7 779,125 + +0.3 779,389 - hcxB NIAGMN_04315 0.21 +0.5 779,389 - hcxB NIAGMN_04315 0.21 -0.9 779,443 - hcxB NIAGMN_04315 0.26 -1.7 779,468 - hcxB NIAGMN_04315 0.28 +0.2 779,662 - hcxB NIAGMN_04315 0.46 -1.4 779,737 - hcxB NIAGMN_04315 0.53 -0.1 779,881 - hcxB NIAGMN_04315 0.66 -1.0 779,922 + hcxB NIAGMN_04315 0.70 +0.5 780,079 + hcxB NIAGMN_04315 0.84 +0.4 780,080 - hcxB NIAGMN_04315 0.84 -0.1 780,080 - hcxB NIAGMN_04315 0.84 -0.1 780,083 + hcxB NIAGMN_04315 0.85 -1.7 780,084 - hcxB NIAGMN_04315 0.85 +0.4 780,205 - +0.2 780,254 - +0.4 780,264 + -3.1 780,264 + -0.0 780,265 - -3.0 780,269 + -0.8 780,270 - +0.5 780,429 - -1.1 780,429 - -0.7 780,651 - ybiB NIAGMN_04320 0.27 -0.0 780,656 - ybiB NIAGMN_04320 0.28 -0.2 780,734 - ybiB NIAGMN_04320 0.36 +3.0 780,734 - ybiB NIAGMN_04320 0.36 +1.8 780,806 + ybiB NIAGMN_04320 0.43 -0.2 780,819 - ybiB NIAGMN_04320 0.45 -1.4 780,887 + ybiB NIAGMN_04320 0.52 -0.6 780,888 - ybiB NIAGMN_04320 0.52 +0.2 780,888 - ybiB NIAGMN_04320 0.52 +1.1
Or see this region's nucleotide sequence