Experiment: Sodium-DL-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ybjL and rcdA are separated by 174 nucleotides rcdA and ybjJ are separated by 83 nucleotides ybjJ and ybjI overlap by 1 nucleotides ybjI and ybjH are separated by 91 nucleotides
NIAGMN_04040: ybjL - aspartate:alanine antiporter, at 725,934 to 727,619
ybjL
NIAGMN_04050: rcdA - DNA-binding transcriptional regulator RcdA, at 727,794 to 728,330
rcdA
NIAGMN_04055: ybjJ - Inner membrane protein YbjJ, at 728,414 to 729,622
ybjJ
NIAGMN_04060: ybjI - 5-amino-6-(5-phospho-D-ribitylamino)uracil phosphatase, at 729,622 to 730,437
ybjI
NIAGMN_04065: ybjH - Uncharacterized protein YbjH, at 730,529 to 730,807
ybjH
Position (kb) 728
729
730 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 727.419 kb on + strand, within ybjL at 727.419 kb on + strand, within ybjL at 727.419 kb on + strand, within ybjL at 727.423 kb on + strand, within ybjL at 727.548 kb on + strand at 727.548 kb on + strand at 727.549 kb on - strand at 727.600 kb on + strand at 727.688 kb on + strand at 727.793 kb on - strand at 727.793 kb on - strand at 727.796 kb on - strand at 727.796 kb on - strand at 727.848 kb on - strand, within rcdA at 727.884 kb on - strand, within rcdA at 728.054 kb on - strand, within rcdA at 728.189 kb on - strand, within rcdA at 728.291 kb on + strand at 728.435 kb on - strand at 728.435 kb on - strand at 728.525 kb on - strand at 728.644 kb on + strand, within ybjJ at 728.691 kb on + strand, within ybjJ at 728.691 kb on + strand, within ybjJ at 728.694 kb on + strand, within ybjJ at 728.694 kb on + strand, within ybjJ at 728.694 kb on + strand, within ybjJ at 728.694 kb on + strand, within ybjJ at 728.695 kb on - strand, within ybjJ at 728.946 kb on + strand, within ybjJ at 728.959 kb on + strand, within ybjJ at 728.959 kb on + strand, within ybjJ at 728.959 kb on + strand, within ybjJ at 728.961 kb on + strand, within ybjJ at 728.961 kb on + strand, within ybjJ at 728.961 kb on + strand, within ybjJ at 728.961 kb on + strand, within ybjJ at 728.963 kb on + strand, within ybjJ at 728.963 kb on + strand, within ybjJ at 728.963 kb on + strand, within ybjJ at 728.963 kb on + strand, within ybjJ at 728.963 kb on + strand, within ybjJ at 728.963 kb on + strand, within ybjJ at 728.963 kb on + strand, within ybjJ at 728.963 kb on + strand, within ybjJ at 728.963 kb on + strand, within ybjJ at 728.963 kb on + strand, within ybjJ at 728.963 kb on + strand, within ybjJ at 728.963 kb on + strand, within ybjJ at 728.963 kb on + strand, within ybjJ at 728.964 kb on - strand, within ybjJ at 728.964 kb on - strand, within ybjJ at 728.964 kb on - strand, within ybjJ at 728.965 kb on + strand, within ybjJ at 728.965 kb on + strand, within ybjJ at 729.126 kb on + strand, within ybjJ at 729.132 kb on + strand, within ybjJ at 729.132 kb on + strand, within ybjJ at 729.279 kb on + strand, within ybjJ at 729.513 kb on + strand at 729.513 kb on + strand at 729.513 kb on + strand at 729.513 kb on + strand at 729.514 kb on - strand at 729.517 kb on + strand at 729.517 kb on + strand at 729.617 kb on - strand at 729.665 kb on + strand at 729.891 kb on + strand, within ybjI at 730.009 kb on + strand, within ybjI at 730.010 kb on - strand, within ybjI at 730.046 kb on + strand, within ybjI at 730.137 kb on + strand, within ybjI at 730.137 kb on + strand, within ybjI at 730.235 kb on + strand, within ybjI at 730.236 kb on - strand, within ybjI at 730.278 kb on + strand, within ybjI at 730.483 kb on + strand at 730.542 kb on + strand at 730.542 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Sodium-DL-Lactate remove 727,419 + ybjL NIAGMN_04040 0.88 +0.5 727,419 + ybjL NIAGMN_04040 0.88 +0.9 727,419 + ybjL NIAGMN_04040 0.88 -1.3 727,423 + ybjL NIAGMN_04040 0.88 +1.8 727,548 + +0.2 727,548 + +0.7 727,549 - +1.8 727,600 + -0.9 727,688 + +0.8 727,793 - -1.6 727,793 - -0.0 727,796 - +0.2 727,796 - +0.8 727,848 - rcdA NIAGMN_04050 0.10 +2.3 727,884 - rcdA NIAGMN_04050 0.17 -1.4 728,054 - rcdA NIAGMN_04050 0.48 -1.3 728,189 - rcdA NIAGMN_04050 0.74 +0.5 728,291 + -0.8 728,435 - -1.2 728,435 - -2.7 728,525 - -1.2 728,644 + ybjJ NIAGMN_04055 0.19 -0.2 728,691 + ybjJ NIAGMN_04055 0.23 +1.4 728,691 + ybjJ NIAGMN_04055 0.23 -2.7 728,694 + ybjJ NIAGMN_04055 0.23 -1.0 728,694 + ybjJ NIAGMN_04055 0.23 +0.3 728,694 + ybjJ NIAGMN_04055 0.23 -1.0 728,694 + ybjJ NIAGMN_04055 0.23 +2.0 728,695 - ybjJ NIAGMN_04055 0.23 -1.1 728,946 + ybjJ NIAGMN_04055 0.44 +0.4 728,959 + ybjJ NIAGMN_04055 0.45 -0.1 728,959 + ybjJ NIAGMN_04055 0.45 +1.1 728,959 + ybjJ NIAGMN_04055 0.45 +0.1 728,961 + ybjJ NIAGMN_04055 0.45 -1.2 728,961 + ybjJ NIAGMN_04055 0.45 +0.9 728,961 + ybjJ NIAGMN_04055 0.45 -0.4 728,961 + ybjJ NIAGMN_04055 0.45 -0.2 728,963 + ybjJ NIAGMN_04055 0.45 -2.6 728,963 + ybjJ NIAGMN_04055 0.45 +2.8 728,963 + ybjJ NIAGMN_04055 0.45 -0.5 728,963 + ybjJ NIAGMN_04055 0.45 -2.5 728,963 + ybjJ NIAGMN_04055 0.45 -0.4 728,963 + ybjJ NIAGMN_04055 0.45 -2.3 728,963 + ybjJ NIAGMN_04055 0.45 +1.3 728,963 + ybjJ NIAGMN_04055 0.45 -2.0 728,963 + ybjJ NIAGMN_04055 0.45 -1.1 728,963 + ybjJ NIAGMN_04055 0.45 -0.1 728,963 + ybjJ NIAGMN_04055 0.45 -0.1 728,963 + ybjJ NIAGMN_04055 0.45 -0.8 728,963 + ybjJ NIAGMN_04055 0.45 -1.0 728,964 - ybjJ NIAGMN_04055 0.45 +0.6 728,964 - ybjJ NIAGMN_04055 0.45 +0.2 728,964 - ybjJ NIAGMN_04055 0.45 -0.1 728,965 + ybjJ NIAGMN_04055 0.46 -1.1 728,965 + ybjJ NIAGMN_04055 0.46 -0.1 729,126 + ybjJ NIAGMN_04055 0.59 +0.0 729,132 + ybjJ NIAGMN_04055 0.59 -0.3 729,132 + ybjJ NIAGMN_04055 0.59 -0.2 729,279 + ybjJ NIAGMN_04055 0.72 +0.2 729,513 + -0.6 729,513 + -0.5 729,513 + +2.5 729,513 + -2.0 729,514 - -0.5 729,517 + +1.0 729,517 + -1.1 729,617 - -1.7 729,665 + -0.3 729,891 + ybjI NIAGMN_04060 0.33 -0.4 730,009 + ybjI NIAGMN_04060 0.47 -1.0 730,010 - ybjI NIAGMN_04060 0.48 -3.1 730,046 + ybjI NIAGMN_04060 0.52 +0.2 730,137 + ybjI NIAGMN_04060 0.63 +0.5 730,137 + ybjI NIAGMN_04060 0.63 +2.0 730,235 + ybjI NIAGMN_04060 0.75 -0.8 730,236 - ybjI NIAGMN_04060 0.75 -0.6 730,278 + ybjI NIAGMN_04060 0.80 -0.6 730,483 + -0.2 730,542 + -0.9 730,542 + -0.2
Or see this region's nucleotide sequence