Experiment: Sodium-DL-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt NIAGMN_02050 and NIAGMN_02055 are separated by 15 nucleotides NIAGMN_02055 and NIAGMN_02060 are separated by 298 nucleotides NIAGMN_02060 and NIAGMN_02065 are separated by 21 nucleotides NIAGMN_02065 and NIAGMN_02070 are separated by 10 nucleotides NIAGMN_02070 and NIAGMN_02075 are separated by 11 nucleotides NIAGMN_02075 and NIAGMN_02080 overlap by 4 nucleotides NIAGMN_02080 and NIAGMN_02085 are separated by 92 nucleotides
NIAGMN_02050: NIAGMN_02050 - Putative transmembrane protein, at 370,100 to 370,750
_02050
NIAGMN_02055: NIAGMN_02055 - transcriptional regulator, at 370,766 to 371,170
_02055
NIAGMN_02060: NIAGMN_02060 - DNA-binding protein, at 371,469 to 371,672
_02060
NIAGMN_02065: NIAGMN_02065 - DUF4761 domain-containing protein, at 371,694 to 372,044
_02065
NIAGMN_02070: NIAGMN_02070 - hypothetical protein, at 372,055 to 372,333
_02070
NIAGMN_02075: NIAGMN_02075 - DUF4754 domain-containing protein, at 372,345 to 372,587
_02075
NIAGMN_02080: NIAGMN_02080 - Membrane protein, at 372,584 to 372,697
_02080
NIAGMN_02085: NIAGMN_02085 - Phage protein, at 372,790 to 373,206
_02085
Position (kb) 371
372
373 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 370.761 kb on + strand at 370.764 kb on + strand at 370.765 kb on - strand at 370.772 kb on - strand at 370.778 kb on - strand at 370.778 kb on - strand at 370.789 kb on - strand at 370.811 kb on - strand, within NIAGMN_02055 at 370.814 kb on - strand, within NIAGMN_02055 at 370.830 kb on - strand, within NIAGMN_02055 at 370.878 kb on - strand, within NIAGMN_02055 at 371.224 kb on + strand at 371.237 kb on + strand at 371.237 kb on + strand at 371.238 kb on - strand at 371.240 kb on - strand at 371.240 kb on - strand at 371.240 kb on - strand at 371.288 kb on - strand at 371.478 kb on - strand at 371.508 kb on + strand, within NIAGMN_02060 at 371.512 kb on + strand, within NIAGMN_02060 at 371.526 kb on + strand, within NIAGMN_02060 at 371.537 kb on - strand, within NIAGMN_02060 at 371.537 kb on - strand, within NIAGMN_02060 at 371.543 kb on + strand, within NIAGMN_02060 at 371.555 kb on + strand, within NIAGMN_02060 at 371.589 kb on - strand, within NIAGMN_02060 at 371.630 kb on - strand, within NIAGMN_02060 at 371.752 kb on + strand, within NIAGMN_02065 at 371.753 kb on - strand, within NIAGMN_02065 at 371.780 kb on + strand, within NIAGMN_02065 at 371.783 kb on + strand, within NIAGMN_02065 at 371.784 kb on - strand, within NIAGMN_02065 at 371.793 kb on + strand, within NIAGMN_02065 at 372.565 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Sodium-DL-Lactate remove 370,761 + +0.3 370,764 + -1.0 370,765 - -1.5 370,772 - +1.0 370,778 - +2.2 370,778 - -0.3 370,789 - +2.5 370,811 - NIAGMN_02055 0.11 -0.4 370,814 - NIAGMN_02055 0.12 -1.4 370,830 - NIAGMN_02055 0.16 +0.1 370,878 - NIAGMN_02055 0.28 -0.3 371,224 + -1.6 371,237 + +1.5 371,237 + +0.4 371,238 - +0.2 371,240 - -1.5 371,240 - -1.0 371,240 - -0.0 371,288 - +0.9 371,478 - -1.0 371,508 + NIAGMN_02060 0.19 +0.9 371,512 + NIAGMN_02060 0.21 -3.1 371,526 + NIAGMN_02060 0.28 +3.1 371,537 - NIAGMN_02060 0.33 +0.7 371,537 - NIAGMN_02060 0.33 +0.2 371,543 + NIAGMN_02060 0.36 +0.5 371,555 + NIAGMN_02060 0.42 -0.2 371,589 - NIAGMN_02060 0.59 +0.4 371,630 - NIAGMN_02060 0.79 +2.1 371,752 + NIAGMN_02065 0.17 +0.4 371,753 - NIAGMN_02065 0.17 +2.4 371,780 + NIAGMN_02065 0.25 -0.7 371,783 + NIAGMN_02065 0.25 -2.4 371,784 - NIAGMN_02065 0.26 +1.3 371,793 + NIAGMN_02065 0.28 -0.2 372,565 - -0.7
Or see this region's nucleotide sequence