Experiment: Sodium-DL-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt xerD and NIAGMN_02045 are separated by 5 nucleotides NIAGMN_02045 and NIAGMN_02050 are separated by 29 nucleotides NIAGMN_02050 and NIAGMN_02055 are separated by 15 nucleotides NIAGMN_02055 and NIAGMN_02060 are separated by 298 nucleotides NIAGMN_02060 and NIAGMN_02065 are separated by 21 nucleotides
NIAGMN_02040: xerD - integrase, at 368,710 to 369,717
xerD
NIAGMN_02045: NIAGMN_02045 - DUF2511 domain-containing protein, at 369,723 to 370,070
_02045
NIAGMN_02050: NIAGMN_02050 - Putative transmembrane protein, at 370,100 to 370,750
_02050
NIAGMN_02055: NIAGMN_02055 - transcriptional regulator, at 370,766 to 371,170
_02055
NIAGMN_02060: NIAGMN_02060 - DNA-binding protein, at 371,469 to 371,672
_02060
NIAGMN_02065: NIAGMN_02065 - DUF4761 domain-containing protein, at 371,694 to 372,044
_02065
Position (kb) 370
371 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 369.194 kb on - strand, within xerD at 369.304 kb on + strand, within xerD at 369.305 kb on - strand, within xerD at 369.305 kb on - strand, within xerD at 369.397 kb on - strand, within xerD at 369.721 kb on - strand at 369.721 kb on - strand at 369.729 kb on - strand at 369.832 kb on - strand, within NIAGMN_02045 at 370.394 kb on + strand, within NIAGMN_02050 at 370.761 kb on + strand at 370.764 kb on + strand at 370.765 kb on - strand at 370.772 kb on - strand at 370.778 kb on - strand at 370.778 kb on - strand at 370.789 kb on - strand at 370.811 kb on - strand, within NIAGMN_02055 at 370.814 kb on - strand, within NIAGMN_02055 at 370.830 kb on - strand, within NIAGMN_02055 at 370.878 kb on - strand, within NIAGMN_02055 at 371.224 kb on + strand at 371.237 kb on + strand at 371.237 kb on + strand at 371.238 kb on - strand at 371.240 kb on - strand at 371.240 kb on - strand at 371.240 kb on - strand at 371.288 kb on - strand at 371.478 kb on - strand at 371.508 kb on + strand, within NIAGMN_02060 at 371.512 kb on + strand, within NIAGMN_02060 at 371.526 kb on + strand, within NIAGMN_02060 at 371.537 kb on - strand, within NIAGMN_02060 at 371.537 kb on - strand, within NIAGMN_02060 at 371.543 kb on + strand, within NIAGMN_02060 at 371.555 kb on + strand, within NIAGMN_02060 at 371.589 kb on - strand, within NIAGMN_02060 at 371.630 kb on - strand, within NIAGMN_02060
Per-strain Table
Position Strand Gene LocusTag Fraction Sodium-DL-Lactate remove 369,194 - xerD NIAGMN_02040 0.48 +1.5 369,304 + xerD NIAGMN_02040 0.59 -0.2 369,305 - xerD NIAGMN_02040 0.59 +0.3 369,305 - xerD NIAGMN_02040 0.59 -1.0 369,397 - xerD NIAGMN_02040 0.68 -0.1 369,721 - -0.5 369,721 - -0.1 369,729 - +1.5 369,832 - NIAGMN_02045 0.31 -0.0 370,394 + NIAGMN_02050 0.45 -1.1 370,761 + +0.3 370,764 + -1.0 370,765 - -1.5 370,772 - +1.0 370,778 - +2.2 370,778 - -0.3 370,789 - +2.5 370,811 - NIAGMN_02055 0.11 -0.4 370,814 - NIAGMN_02055 0.12 -1.4 370,830 - NIAGMN_02055 0.16 +0.1 370,878 - NIAGMN_02055 0.28 -0.3 371,224 + -1.6 371,237 + +1.5 371,237 + +0.4 371,238 - +0.2 371,240 - -1.5 371,240 - -1.0 371,240 - -0.0 371,288 - +0.9 371,478 - -1.0 371,508 + NIAGMN_02060 0.19 +0.9 371,512 + NIAGMN_02060 0.21 -3.1 371,526 + NIAGMN_02060 0.28 +3.1 371,537 - NIAGMN_02060 0.33 +0.7 371,537 - NIAGMN_02060 0.33 +0.2 371,543 + NIAGMN_02060 0.36 +0.5 371,555 + NIAGMN_02060 0.42 -0.2 371,589 - NIAGMN_02060 0.59 +0.4 371,630 - NIAGMN_02060 0.79 +2.1
Or see this region's nucleotide sequence