Experiment: Sodium-DL-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yobB and exoX are separated by 23 nucleotides exoX and ptrB overlap by 4 nucleotides ptrB and yebE are separated by 208 nucleotides yebE and NIAGMN_01690 are separated by 59 nucleotides
NIAGMN_01670: yobB - Uncharacterized protein YobB, at 302,853 to 303,509
yobB
NIAGMN_01675: exoX - exodeoxyribonuclease X, at 303,533 to 304,195
exoX
NIAGMN_01680: ptrB - oligopeptidase B, at 304,192 to 306,252
ptrB
NIAGMN_01685: yebE - Inner membrane protein YebE, at 306,461 to 307,120
yebE
NIAGMN_01690: NIAGMN_01690 - hypothetical protein, at 307,180 to 307,290
_01690
Position (kb) 304
305
306
307 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 303.454 kb on + strand at 303.658 kb on + strand, within exoX at 303.743 kb on + strand, within exoX at 303.885 kb on - strand, within exoX at 303.906 kb on + strand, within exoX at 303.953 kb on + strand, within exoX at 303.956 kb on - strand, within exoX at 304.193 kb on + strand at 304.268 kb on + strand at 304.318 kb on - strand at 304.388 kb on + strand at 304.479 kb on + strand, within ptrB at 304.705 kb on + strand, within ptrB at 304.743 kb on + strand, within ptrB at 304.823 kb on + strand, within ptrB at 304.858 kb on - strand, within ptrB at 304.890 kb on - strand, within ptrB at 305.051 kb on - strand, within ptrB at 305.366 kb on + strand, within ptrB at 305.366 kb on + strand, within ptrB at 305.366 kb on + strand, within ptrB at 305.543 kb on - strand, within ptrB at 305.690 kb on + strand, within ptrB at 305.691 kb on - strand, within ptrB at 305.964 kb on + strand, within ptrB at 305.970 kb on - strand, within ptrB at 305.988 kb on - strand, within ptrB at 306.021 kb on - strand, within ptrB at 306.117 kb on - strand at 306.282 kb on - strand at 306.284 kb on - strand at 306.284 kb on - strand at 306.349 kb on + strand at 306.350 kb on - strand at 307.145 kb on - strand at 307.159 kb on - strand at 307.183 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Sodium-DL-Lactate remove 303,454 + -1.0 303,658 + exoX NIAGMN_01675 0.19 -0.3 303,743 + exoX NIAGMN_01675 0.32 +1.2 303,885 - exoX NIAGMN_01675 0.53 +1.4 303,906 + exoX NIAGMN_01675 0.56 -0.6 303,953 + exoX NIAGMN_01675 0.63 -0.1 303,956 - exoX NIAGMN_01675 0.64 -1.2 304,193 + +1.1 304,268 + -0.1 304,318 - -1.6 304,388 + -0.5 304,479 + ptrB NIAGMN_01680 0.14 +0.9 304,705 + ptrB NIAGMN_01680 0.25 -1.2 304,743 + ptrB NIAGMN_01680 0.27 -0.5 304,823 + ptrB NIAGMN_01680 0.31 +0.5 304,858 - ptrB NIAGMN_01680 0.32 +2.0 304,890 - ptrB NIAGMN_01680 0.34 -0.4 305,051 - ptrB NIAGMN_01680 0.42 +3.4 305,366 + ptrB NIAGMN_01680 0.57 +0.3 305,366 + ptrB NIAGMN_01680 0.57 +1.4 305,366 + ptrB NIAGMN_01680 0.57 +1.4 305,543 - ptrB NIAGMN_01680 0.66 -3.7 305,690 + ptrB NIAGMN_01680 0.73 -0.3 305,691 - ptrB NIAGMN_01680 0.73 +0.4 305,964 + ptrB NIAGMN_01680 0.86 -1.7 305,970 - ptrB NIAGMN_01680 0.86 -0.7 305,988 - ptrB NIAGMN_01680 0.87 +0.7 306,021 - ptrB NIAGMN_01680 0.89 +0.5 306,117 - +0.7 306,282 - -0.1 306,284 - +0.5 306,284 - -0.6 306,349 + +0.8 306,350 - -0.8 307,145 - +0.4 307,159 - +0.6 307,183 + -0.2
Or see this region's nucleotide sequence