Experiment: Guanine 0.6617 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt A4249_RS16000 and A4249_RS16005 are separated by 78 nucleotides A4249_RS16005 and A4249_RS16010 are separated by 114 nucleotides A4249_RS16010 and A4249_RS16015 overlap by 4 nucleotides A4249_RS16015 and A4249_RS16020 are separated by 145 nucleotides A4249_RS16020 and A4249_RS16025 are separated by 13 nucleotides
A4249_RS16000: A4249_RS16000 - hypothetical protein, at 3,225,421 to 3,228,270
_RS16000
A4249_RS16005: A4249_RS16005 - HU family DNA-binding protein, at 3,228,349 to 3,228,627
_RS16005
A4249_RS16010: A4249_RS16010 - flagellar type III secretion system pore protein FliP, at 3,228,742 to 3,229,554
_RS16010
A4249_RS16015: A4249_RS16015 - flagellar biosynthetic protein FliO, at 3,229,551 to 3,229,841
_RS16015
A4249_RS16020: A4249_RS16020 - flagellar basal body rod protein FlgB, at 3,229,987 to 3,230,391
_RS16020
A4249_RS16025: A4249_RS16025 - flagellar basal body rod protein FlgC, at 3,230,405 to 3,230,824
_RS16025
Position (kb)
3228
3229
3230 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3227.944 kb on + strand, within A4249_RS16000 at 3227.944 kb on + strand, within A4249_RS16000 at 3227.944 kb on + strand, within A4249_RS16000 at 3227.945 kb on - strand, within A4249_RS16000 at 3227.945 kb on - strand, within A4249_RS16000 at 3227.945 kb on - strand, within A4249_RS16000 at 3227.945 kb on - strand, within A4249_RS16000 at 3227.945 kb on - strand, within A4249_RS16000 at 3227.968 kb on + strand, within A4249_RS16000 at 3227.968 kb on + strand, within A4249_RS16000 at 3227.969 kb on - strand, within A4249_RS16000 at 3227.969 kb on - strand, within A4249_RS16000 at 3228.720 kb on + strand at 3228.721 kb on - strand at 3228.728 kb on + strand at 3228.729 kb on - strand at 3228.744 kb on - strand at 3229.169 kb on + strand, within A4249_RS16010 at 3229.170 kb on - strand, within A4249_RS16010 at 3229.170 kb on - strand, within A4249_RS16010 at 3229.754 kb on - strand, within A4249_RS16015 at 3229.754 kb on - strand, within A4249_RS16015 at 3229.924 kb on + strand at 3230.003 kb on + strand at 3230.004 kb on - strand at 3230.089 kb on - strand, within A4249_RS16020 at 3230.089 kb on - strand, within A4249_RS16020 at 3230.190 kb on + strand, within A4249_RS16020 at 3230.191 kb on - strand, within A4249_RS16020 at 3230.317 kb on + strand, within A4249_RS16020 at 3230.317 kb on + strand, within A4249_RS16020 at 3230.317 kb on + strand, within A4249_RS16020 at 3230.317 kb on + strand, within A4249_RS16020 at 3230.318 kb on - strand, within A4249_RS16020 at 3230.318 kb on - strand, within A4249_RS16020 at 3230.318 kb on - strand, within A4249_RS16020 at 3230.318 kb on - strand, within A4249_RS16020 at 3230.318 kb on - strand, within A4249_RS16020 at 3230.318 kb on - strand, within A4249_RS16020 at 3230.318 kb on - strand, within A4249_RS16020 at 3230.318 kb on - strand, within A4249_RS16020 at 3230.318 kb on - strand, within A4249_RS16020 at 3230.338 kb on + strand, within A4249_RS16020 at 3230.338 kb on + strand, within A4249_RS16020 at 3230.338 kb on + strand, within A4249_RS16020 at 3230.338 kb on + strand, within A4249_RS16020 at 3230.339 kb on - strand, within A4249_RS16020 at 3230.402 kb on + strand at 3230.402 kb on + strand at 3230.402 kb on + strand at 3230.402 kb on + strand at 3230.402 kb on + strand at 3230.402 kb on + strand at 3230.402 kb on + strand at 3230.402 kb on + strand at 3230.402 kb on + strand at 3230.403 kb on - strand at 3230.403 kb on - strand at 3230.403 kb on - strand at 3230.403 kb on - strand at 3230.403 kb on - strand at 3230.403 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Guanine 0.6617 mM (C) remove 3,227,944 + A4249_RS16000 0.89 -0.4 3,227,944 + A4249_RS16000 0.89 -0.6 3,227,944 + A4249_RS16000 0.89 +0.1 3,227,945 - A4249_RS16000 0.89 -0.0 3,227,945 - A4249_RS16000 0.89 -0.3 3,227,945 - A4249_RS16000 0.89 +1.5 3,227,945 - A4249_RS16000 0.89 +0.0 3,227,945 - A4249_RS16000 0.89 -0.2 3,227,968 + A4249_RS16000 0.89 +0.9 3,227,968 + A4249_RS16000 0.89 +0.5 3,227,969 - A4249_RS16000 0.89 +0.6 3,227,969 - A4249_RS16000 0.89 +0.2 3,228,720 + -0.6 3,228,721 - -0.4 3,228,728 + +0.1 3,228,729 - +1.9 3,228,744 - -0.5 3,229,169 + A4249_RS16010 0.53 +1.4 3,229,170 - A4249_RS16010 0.53 -0.3 3,229,170 - A4249_RS16010 0.53 +0.1 3,229,754 - A4249_RS16015 0.70 +0.6 3,229,754 - A4249_RS16015 0.70 +1.1 3,229,924 + -1.3 3,230,003 + +0.6 3,230,004 - -0.3 3,230,089 - A4249_RS16020 0.25 -0.7 3,230,089 - A4249_RS16020 0.25 -0.2 3,230,190 + A4249_RS16020 0.50 -0.5 3,230,191 - A4249_RS16020 0.50 +0.8 3,230,317 + A4249_RS16020 0.81 +0.4 3,230,317 + A4249_RS16020 0.81 -0.2 3,230,317 + A4249_RS16020 0.81 -0.4 3,230,317 + A4249_RS16020 0.81 -0.1 3,230,318 - A4249_RS16020 0.82 -0.8 3,230,318 - A4249_RS16020 0.82 -1.6 3,230,318 - A4249_RS16020 0.82 +0.4 3,230,318 - A4249_RS16020 0.82 -0.6 3,230,318 - A4249_RS16020 0.82 +0.2 3,230,318 - A4249_RS16020 0.82 +0.7 3,230,318 - A4249_RS16020 0.82 -1.0 3,230,318 - A4249_RS16020 0.82 +0.2 3,230,318 - A4249_RS16020 0.82 +0.2 3,230,338 + A4249_RS16020 0.87 +0.2 3,230,338 + A4249_RS16020 0.87 +0.1 3,230,338 + A4249_RS16020 0.87 -0.2 3,230,338 + A4249_RS16020 0.87 -0.6 3,230,339 - A4249_RS16020 0.87 +0.5 3,230,402 + -0.9 3,230,402 + +0.5 3,230,402 + +1.2 3,230,402 + -0.2 3,230,402 + -0.7 3,230,402 + +0.3 3,230,402 + +0.1 3,230,402 + -0.6 3,230,402 + -0.4 3,230,403 - -0.8 3,230,403 - +0.1 3,230,403 - -0.2 3,230,403 - -0.2 3,230,403 - -2.2 3,230,403 - +0.1
Or see this region's nucleotide sequence