Strain Fitness in Escherichia coli ECRC101 around MCAODC_22855

Experiment: Sodium-DL-Lactate

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntcomR and ycfJ are separated by 61 nucleotidesycfJ and ndh are separated by 226 nucleotidesndh and ycfP are separated by 399 nucleotidesycfP and nagZ are separated by 22 nucleotides MCAODC_22845: comR - TetR family copper-responsive transcriptional repressor ComR, at 1,312,460 to 1,313,155 comR MCAODC_22850: ycfJ - Uncharacterized protein YcfJ, at 1,313,217 to 1,313,756 ycfJ MCAODC_22855: ndh - NADH-quinone dehydrogenase, at 1,313,983 to 1,315,287 ndh MCAODC_22860: ycfP - alpha/beta hydrolase YcfP, at 1,315,687 to 1,316,229 ycfP MCAODC_22865: nagZ - beta-N-acetylhexosaminidase, at 1,316,252 to 1,317,277 nagZ Position (kb) 1313 1314 1315 1316Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 1313.044 kb on + strand, within comRat 1313.050 kb on + strand, within comRat 1313.051 kb on - strand, within comRat 1313.053 kb on + strand, within comRat 1313.208 kb on - strandat 1313.208 kb on - strandat 1313.218 kb on + strandat 1313.220 kb on + strandat 1313.253 kb on - strandat 1313.321 kb on - strand, within ycfJat 1313.360 kb on - strand, within ycfJat 1313.393 kb on - strand, within ycfJat 1313.750 kb on + strandat 1313.763 kb on - strandat 1313.780 kb on + strandat 1313.780 kb on + strandat 1313.815 kb on - strandat 1313.854 kb on - strandat 1313.857 kb on + strandat 1313.976 kb on - strandat 1313.976 kb on - strandat 1314.021 kb on + strandat 1314.021 kb on + strandat 1314.052 kb on - strandat 1314.057 kb on - strandat 1314.097 kb on - strandat 1314.098 kb on + strandat 1314.098 kb on + strandat 1314.099 kb on - strandat 1314.099 kb on - strandat 1314.099 kb on - strandat 1314.099 kb on - strandat 1314.099 kb on - strandat 1314.099 kb on - strandat 1314.099 kb on - strandat 1314.101 kb on - strandat 1314.352 kb on - strand, within ndhat 1314.356 kb on + strand, within ndhat 1314.356 kb on + strand, within ndhat 1314.357 kb on - strand, within ndhat 1314.476 kb on - strand, within ndhat 1314.497 kb on + strand, within ndhat 1314.606 kb on + strand, within ndhat 1314.606 kb on + strand, within ndhat 1314.606 kb on + strand, within ndhat 1314.630 kb on + strand, within ndhat 1314.646 kb on + strand, within ndhat 1314.647 kb on - strand, within ndhat 1314.656 kb on - strand, within ndhat 1314.945 kb on - strand, within ndhat 1315.030 kb on - strand, within ndhat 1315.210 kb on - strandat 1315.282 kb on - strandat 1315.282 kb on - strandat 1315.282 kb on - strandat 1315.338 kb on + strandat 1315.339 kb on - strandat 1315.530 kb on + strandat 1315.530 kb on + strandat 1315.544 kb on + strandat 1315.544 kb on + strandat 1315.544 kb on + strandat 1315.544 kb on + strandat 1315.544 kb on + strandat 1315.544 kb on + strandat 1315.544 kb on + strandat 1315.544 kb on + strandat 1315.667 kb on - strandat 1315.710 kb on + strandat 1315.711 kb on - strandat 1315.735 kb on - strandat 1315.831 kb on - strand, within ycfPat 1315.890 kb on + strand, within ycfPat 1315.891 kb on - strand, within ycfPat 1315.891 kb on - strand, within ycfPat 1315.907 kb on + strand, within ycfPat 1315.959 kb on + strand, within ycfPat 1315.960 kb on - strand, within ycfPat 1316.104 kb on - strand, within ycfPat 1316.104 kb on - strand, within ycfPat 1316.104 kb on - strand, within ycfPat 1316.135 kb on - strand, within ycfPat 1316.144 kb on - strand, within ycfPat 1316.171 kb on - strand, within ycfPat 1316.219 kb on + strandat 1316.223 kb on + strandat 1316.223 kb on + strandat 1316.224 kb on - strandat 1316.242 kb on + strandat 1316.242 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Sodium-DL-Lactate
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1,313,044 + comR MCAODC_22845 0.84 +1.0
1,313,050 + comR MCAODC_22845 0.85 -0.3
1,313,051 - comR MCAODC_22845 0.85 +1.2
1,313,053 + comR MCAODC_22845 0.85 -0.2
1,313,208 - -0.0
1,313,208 - +0.3
1,313,218 + -1.4
1,313,220 + -0.3
1,313,253 - +0.1
1,313,321 - ycfJ MCAODC_22850 0.19 +1.0
1,313,360 - ycfJ MCAODC_22850 0.26 +0.3
1,313,393 - ycfJ MCAODC_22850 0.33 -1.6
1,313,750 + -0.2
1,313,763 - +1.0
1,313,780 + +0.2
1,313,780 + -0.4
1,313,815 - +0.1
1,313,854 - +0.4
1,313,857 + -4.6
1,313,976 - -0.0
1,313,976 - +1.1
1,314,021 + -1.3
1,314,021 + +1.2
1,314,052 - +1.5
1,314,057 - -1.0
1,314,097 - +1.0
1,314,098 + -0.4
1,314,098 + -2.9
1,314,099 - -1.5
1,314,099 - +1.1
1,314,099 - +1.9
1,314,099 - -1.8
1,314,099 - -0.8
1,314,099 - +2.5
1,314,099 - -1.5
1,314,101 - +0.3
1,314,352 - ndh MCAODC_22855 0.28 +0.4
1,314,356 + ndh MCAODC_22855 0.29 -0.5
1,314,356 + ndh MCAODC_22855 0.29 +0.2
1,314,357 - ndh MCAODC_22855 0.29 +0.5
1,314,476 - ndh MCAODC_22855 0.38 -1.6
1,314,497 + ndh MCAODC_22855 0.39 +1.7
1,314,606 + ndh MCAODC_22855 0.48 -2.7
1,314,606 + ndh MCAODC_22855 0.48 +0.8
1,314,606 + ndh MCAODC_22855 0.48 +0.4
1,314,630 + ndh MCAODC_22855 0.50 +1.7
1,314,646 + ndh MCAODC_22855 0.51 -0.0
1,314,647 - ndh MCAODC_22855 0.51 +0.7
1,314,656 - ndh MCAODC_22855 0.52 +0.7
1,314,945 - ndh MCAODC_22855 0.74 +0.1
1,315,030 - ndh MCAODC_22855 0.80 -0.2
1,315,210 - +0.7
1,315,282 - +0.4
1,315,282 - -0.1
1,315,282 - +0.7
1,315,338 + +0.4
1,315,339 - +0.3
1,315,530 + +0.3
1,315,530 + +0.6
1,315,544 + +1.0
1,315,544 + +0.8
1,315,544 + -2.1
1,315,544 + -1.2
1,315,544 + +0.4
1,315,544 + +0.1
1,315,544 + +0.1
1,315,544 + +0.7
1,315,667 - +1.4
1,315,710 + -0.1
1,315,711 - +0.3
1,315,735 - +2.0
1,315,831 - ycfP MCAODC_22860 0.27 -0.2
1,315,890 + ycfP MCAODC_22860 0.37 -0.2
1,315,891 - ycfP MCAODC_22860 0.38 -0.8
1,315,891 - ycfP MCAODC_22860 0.38 -1.1
1,315,907 + ycfP MCAODC_22860 0.41 -1.9
1,315,959 + ycfP MCAODC_22860 0.50 -0.2
1,315,960 - ycfP MCAODC_22860 0.50 -0.7
1,316,104 - ycfP MCAODC_22860 0.77 +2.3
1,316,104 - ycfP MCAODC_22860 0.77 -0.1
1,316,104 - ycfP MCAODC_22860 0.77 -1.2
1,316,135 - ycfP MCAODC_22860 0.83 +1.3
1,316,144 - ycfP MCAODC_22860 0.84 +0.5
1,316,171 - ycfP MCAODC_22860 0.89 -1.2
1,316,219 + -3.2
1,316,223 + -0.6
1,316,223 + -0.4
1,316,224 - -1.1
1,316,242 + -0.0
1,316,242 + +2.7

Or see this region's nucleotide sequence