Strain Fitness in Escherichia coli ECRC101 around MCAODC_21805

Experiment: Sodium-DL-Lactate

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntsirB1 and sirB2 are separated by 3 nucleotidessirB2 and prmC overlap by 4 nucleotidesprmC and prfA overlap by 1 nucleotides MCAODC_21795: sirB1 - invasion regulator SirB1, at 1,141,843 to 1,142,652 sirB1 MCAODC_21800: sirB2 - invasion regulator SirB2, at 1,142,656 to 1,143,048 sirB2 MCAODC_21805: prmC - peptide chain release factor N(5)-glutamine methyltransferase, at 1,143,045 to 1,143,878 prmC MCAODC_21810: prfA - peptide chain release factor 1, at 1,143,878 to 1,144,960 prfA Position (kb) 1143 1144Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3at 1142.117 kb on - strand, within sirB1at 1142.151 kb on - strand, within sirB1at 1142.192 kb on - strand, within sirB1at 1142.203 kb on - strand, within sirB1at 1142.350 kb on + strand, within sirB1at 1142.351 kb on - strand, within sirB1at 1142.351 kb on - strand, within sirB1at 1142.692 kb on - strandat 1142.692 kb on - strandat 1142.702 kb on + strand, within sirB2at 1142.703 kb on - strand, within sirB2at 1142.703 kb on - strand, within sirB2at 1142.779 kb on - strand, within sirB2at 1142.796 kb on - strand, within sirB2at 1142.796 kb on - strand, within sirB2at 1142.803 kb on - strand, within sirB2at 1142.893 kb on + strand, within sirB2at 1142.894 kb on - strand, within sirB2at 1142.966 kb on - strand, within sirB2at 1142.978 kb on - strand, within sirB2at 1142.984 kb on - strand, within sirB2at 1142.984 kb on - strand, within sirB2at 1143.012 kb on + strandat 1143.013 kb on - strandat 1143.013 kb on - strandat 1143.013 kb on - strandat 1143.013 kb on - strandat 1143.013 kb on - strandat 1143.013 kb on - strandat 1143.426 kb on - strand, within prmCat 1143.667 kb on - strand, within prmCat 1143.765 kb on - strand, within prmCat 1143.929 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Sodium-DL-Lactate
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1,142,117 - sirB1 MCAODC_21795 0.34 -0.0
1,142,151 - sirB1 MCAODC_21795 0.38 -0.7
1,142,192 - sirB1 MCAODC_21795 0.43 -2.0
1,142,203 - sirB1 MCAODC_21795 0.44 -0.6
1,142,350 + sirB1 MCAODC_21795 0.63 +0.6
1,142,351 - sirB1 MCAODC_21795 0.63 +2.7
1,142,351 - sirB1 MCAODC_21795 0.63 +3.2
1,142,692 - -1.6
1,142,692 - -0.9
1,142,702 + sirB2 MCAODC_21800 0.12 -2.2
1,142,703 - sirB2 MCAODC_21800 0.12 +0.4
1,142,703 - sirB2 MCAODC_21800 0.12 +1.6
1,142,779 - sirB2 MCAODC_21800 0.31 -2.7
1,142,796 - sirB2 MCAODC_21800 0.36 -0.8
1,142,796 - sirB2 MCAODC_21800 0.36 -0.9
1,142,803 - sirB2 MCAODC_21800 0.37 -0.3
1,142,893 + sirB2 MCAODC_21800 0.60 -4.1
1,142,894 - sirB2 MCAODC_21800 0.61 +1.1
1,142,966 - sirB2 MCAODC_21800 0.79 +0.5
1,142,978 - sirB2 MCAODC_21800 0.82 +0.7
1,142,984 - sirB2 MCAODC_21800 0.83 -0.8
1,142,984 - sirB2 MCAODC_21800 0.83 +1.2
1,143,012 + +1.0
1,143,013 - +1.2
1,143,013 - +0.4
1,143,013 - -0.3
1,143,013 - -1.1
1,143,013 - +0.2
1,143,013 - +0.8
1,143,426 - prmC MCAODC_21805 0.46 -3.7
1,143,667 - prmC MCAODC_21805 0.75 -0.6
1,143,765 - prmC MCAODC_21805 0.86 -0.3
1,143,929 - -0.2

Or see this region's nucleotide sequence