Experiment: Sodium-DL-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Arg_trna and Ile2_trna are separated by 80 nucleotides Ile2_trna and yhdJ are separated by 40 nucleotides yhdJ and MCAODC_21445 are separated by 150 nucleotides MCAODC_21445 and MCAODC_21450 are separated by 241 nucleotides
MCAODC_21430: Arg_trna - tRNA-Arg(tct), at 1,081,595 to 1,081,671
_trna
MCAODC_21435: Ile2_trna - tRNA-Ile2(cat), at 1,081,752 to 1,081,827
_trna
MCAODC_21440: yhdJ - DNA adenine methylase, at 1,081,868 to 1,082,926
yhdJ
MCAODC_21445: MCAODC_21445 - TrmB family transcriptional regulator, at 1,083,077 to 1,083,274
_21445
MCAODC_21450: MCAODC_21450 - DUF1133 domain-containing protein, at 1,083,516 to 1,084,046
_21450
Position (kb)
1081
1082
1083 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1081.062 kb on + strand at 1081.062 kb on + strand at 1081.072 kb on + strand at 1081.192 kb on + strand at 1081.195 kb on - strand at 1081.237 kb on + strand at 1081.237 kb on + strand at 1081.237 kb on + strand at 1081.238 kb on - strand at 1081.315 kb on - strand at 1081.315 kb on - strand at 1081.315 kb on - strand at 1081.315 kb on - strand at 1081.315 kb on - strand at 1081.315 kb on - strand at 1081.321 kb on - strand at 1081.330 kb on - strand at 1081.331 kb on + strand at 1081.426 kb on - strand at 1081.743 kb on - strand at 1081.839 kb on - strand at 1081.886 kb on + strand at 1082.092 kb on - strand, within yhdJ at 1082.094 kb on + strand, within yhdJ at 1082.108 kb on - strand, within yhdJ at 1082.109 kb on + strand, within yhdJ at 1082.110 kb on - strand, within yhdJ at 1082.110 kb on - strand, within yhdJ at 1082.127 kb on + strand, within yhdJ at 1082.170 kb on + strand, within yhdJ at 1082.171 kb on - strand, within yhdJ at 1082.171 kb on - strand, within yhdJ at 1082.172 kb on + strand, within yhdJ at 1082.173 kb on - strand, within yhdJ at 1082.173 kb on - strand, within yhdJ at 1082.214 kb on + strand, within yhdJ at 1082.214 kb on + strand, within yhdJ at 1082.214 kb on + strand, within yhdJ at 1082.215 kb on - strand, within yhdJ at 1082.215 kb on - strand, within yhdJ at 1082.219 kb on - strand, within yhdJ at 1082.219 kb on - strand, within yhdJ at 1082.433 kb on + strand, within yhdJ at 1082.434 kb on - strand, within yhdJ at 1082.434 kb on - strand, within yhdJ at 1082.473 kb on + strand, within yhdJ at 1082.621 kb on - strand, within yhdJ at 1082.892 kb on - strand at 1082.921 kb on - strand at 1082.949 kb on + strand at 1082.949 kb on + strand at 1083.037 kb on - strand at 1083.037 kb on - strand at 1083.112 kb on - strand, within MCAODC_21445 at 1083.353 kb on - strand at 1083.353 kb on - strand at 1083.387 kb on - strand at 1083.457 kb on - strand at 1083.541 kb on - strand at 1083.544 kb on + strand at 1083.545 kb on - strand at 1083.545 kb on - strand at 1083.545 kb on - strand at 1083.545 kb on - strand at 1083.651 kb on - strand, within MCAODC_21450 at 1083.671 kb on - strand, within MCAODC_21450 at 1083.687 kb on - strand, within MCAODC_21450 at 1083.758 kb on + strand, within MCAODC_21450 at 1083.759 kb on - strand, within MCAODC_21450 at 1083.867 kb on - strand, within MCAODC_21450
Per-strain Table
Position Strand Gene LocusTag Fraction Sodium-DL-Lactate remove 1,081,062 + +0.7 1,081,062 + -1.0 1,081,072 + +0.5 1,081,192 + +1.2 1,081,195 - -1.9 1,081,237 + -1.1 1,081,237 + +0.8 1,081,237 + -0.7 1,081,238 - -0.6 1,081,315 - -0.2 1,081,315 - +0.8 1,081,315 - +0.3 1,081,315 - +0.4 1,081,315 - -1.5 1,081,315 - +0.5 1,081,321 - +0.2 1,081,330 - +0.4 1,081,331 + +1.4 1,081,426 - +0.0 1,081,743 - -0.9 1,081,839 - -0.6 1,081,886 + +1.2 1,082,092 - yhdJ MCAODC_21440 0.21 +1.0 1,082,094 + yhdJ MCAODC_21440 0.21 -1.2 1,082,108 - yhdJ MCAODC_21440 0.23 +1.0 1,082,109 + yhdJ MCAODC_21440 0.23 -1.3 1,082,110 - yhdJ MCAODC_21440 0.23 -0.0 1,082,110 - yhdJ MCAODC_21440 0.23 -0.2 1,082,127 + yhdJ MCAODC_21440 0.24 +0.4 1,082,170 + yhdJ MCAODC_21440 0.29 +1.1 1,082,171 - yhdJ MCAODC_21440 0.29 +0.2 1,082,171 - yhdJ MCAODC_21440 0.29 -0.1 1,082,172 + yhdJ MCAODC_21440 0.29 -1.9 1,082,173 - yhdJ MCAODC_21440 0.29 -0.6 1,082,173 - yhdJ MCAODC_21440 0.29 -0.2 1,082,214 + yhdJ MCAODC_21440 0.33 +1.8 1,082,214 + yhdJ MCAODC_21440 0.33 -0.3 1,082,214 + yhdJ MCAODC_21440 0.33 +3.3 1,082,215 - yhdJ MCAODC_21440 0.33 +0.3 1,082,215 - yhdJ MCAODC_21440 0.33 +0.4 1,082,219 - yhdJ MCAODC_21440 0.33 +0.9 1,082,219 - yhdJ MCAODC_21440 0.33 -1.6 1,082,433 + yhdJ MCAODC_21440 0.53 -1.3 1,082,434 - yhdJ MCAODC_21440 0.53 +0.6 1,082,434 - yhdJ MCAODC_21440 0.53 -0.8 1,082,473 + yhdJ MCAODC_21440 0.57 +0.5 1,082,621 - yhdJ MCAODC_21440 0.71 -0.1 1,082,892 - +0.7 1,082,921 - +0.1 1,082,949 + +0.7 1,082,949 + +0.6 1,083,037 - +0.8 1,083,037 - +1.5 1,083,112 - MCAODC_21445 0.18 +3.5 1,083,353 - +0.2 1,083,353 - -1.1 1,083,387 - -0.7 1,083,457 - -0.4 1,083,541 - -1.4 1,083,544 + +1.6 1,083,545 - -0.2 1,083,545 - +1.5 1,083,545 - +1.2 1,083,545 - -0.1 1,083,651 - MCAODC_21450 0.25 -0.5 1,083,671 - MCAODC_21450 0.29 +1.8 1,083,687 - MCAODC_21450 0.32 -0.0 1,083,758 + MCAODC_21450 0.46 +1.1 1,083,759 - MCAODC_21450 0.46 -0.7 1,083,867 - MCAODC_21450 0.66 -0.7
Or see this region's nucleotide sequence