Strain Fitness in Escherichia coli ECRC101 around MCAODC_19630

Experiment: Sodium-DL-Lactate

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntfliD and fliS are separated by 24 nucleotidesfliS and fliT overlap by 1 nucleotidesfliT and amyA are separated by 77 nucleotides MCAODC_19620: fliD - flagellar filament capping protein FliD, at 755,673 to 757,070 fliD MCAODC_19625: fliS - flagellar export chaperone FliS, at 757,095 to 757,505 fliS MCAODC_19630: fliT - flagella biosynthesis regulatory protein FliT, at 757,505 to 757,870 fliT MCAODC_19635: amyA - alpha-amylase, at 757,948 to 759,435 amyA Position (kb) 757 758Strain fitness (log2 ratio) -2 -1 0 1 2 3at 756.510 kb on - strand, within fliDat 756.520 kb on + strand, within fliDat 756.525 kb on - strand, within fliDat 756.525 kb on - strand, within fliDat 756.653 kb on + strand, within fliDat 756.656 kb on + strand, within fliDat 756.656 kb on + strand, within fliDat 756.656 kb on + strand, within fliDat 756.656 kb on + strand, within fliDat 756.675 kb on + strand, within fliDat 756.781 kb on + strand, within fliDat 756.920 kb on + strand, within fliDat 756.921 kb on - strand, within fliDat 757.029 kb on + strandat 757.034 kb on - strandat 757.058 kb on + strandat 757.058 kb on + strandat 757.098 kb on + strandat 757.122 kb on + strandat 757.122 kb on + strandat 757.359 kb on + strand, within fliSat 757.359 kb on + strand, within fliSat 757.361 kb on + strand, within fliSat 757.361 kb on + strand, within fliSat 757.361 kb on + strand, within fliSat 757.361 kb on + strand, within fliSat 757.361 kb on + strand, within fliSat 757.362 kb on - strand, within fliSat 757.362 kb on - strand, within fliSat 757.365 kb on + strand, within fliSat 757.365 kb on + strand, within fliSat 757.365 kb on + strand, within fliSat 757.365 kb on + strand, within fliSat 757.367 kb on + strand, within fliSat 757.367 kb on + strand, within fliSat 757.435 kb on + strand, within fliSat 757.445 kb on + strand, within fliSat 757.524 kb on + strandat 757.527 kb on - strandat 757.569 kb on + strand, within fliTat 757.622 kb on + strand, within fliTat 757.622 kb on + strand, within fliTat 757.622 kb on + strand, within fliTat 757.622 kb on + strand, within fliTat 757.623 kb on - strand, within fliTat 757.737 kb on + strand, within fliTat 757.737 kb on + strand, within fliTat 757.738 kb on - strand, within fliTat 757.920 kb on + strandat 757.920 kb on + strandat 757.920 kb on + strandat 757.920 kb on + strandat 757.920 kb on + strandat 757.921 kb on - strandat 757.954 kb on - strandat 757.968 kb on - strandat 757.968 kb on - strandat 757.968 kb on - strandat 757.987 kb on + strandat 758.052 kb on + strandat 758.080 kb on + strandat 758.123 kb on - strand, within amyAat 758.129 kb on + strand, within amyAat 758.252 kb on + strand, within amyAat 758.252 kb on + strand, within amyAat 758.289 kb on + strand, within amyAat 758.406 kb on + strand, within amyAat 758.427 kb on + strand, within amyAat 758.473 kb on - strand, within amyAat 758.599 kb on + strand, within amyAat 758.599 kb on + strand, within amyAat 758.599 kb on + strand, within amyAat 758.599 kb on + strand, within amyAat 758.664 kb on + strand, within amyAat 758.680 kb on + strand, within amyAat 758.702 kb on + strand, within amyAat 758.702 kb on + strand, within amyAat 758.730 kb on + strand, within amyAat 758.730 kb on + strand, within amyAat 758.776 kb on + strand, within amyAat 758.777 kb on - strand, within amyA

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Per-strain Table

Position Strand Gene LocusTag Fraction Sodium-DL-Lactate
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756,510 - fliD MCAODC_19620 0.60 -0.1
756,520 + fliD MCAODC_19620 0.61 -0.2
756,525 - fliD MCAODC_19620 0.61 +1.1
756,525 - fliD MCAODC_19620 0.61 -1.6
756,653 + fliD MCAODC_19620 0.70 -0.9
756,656 + fliD MCAODC_19620 0.70 -0.1
756,656 + fliD MCAODC_19620 0.70 -0.4
756,656 + fliD MCAODC_19620 0.70 -0.9
756,656 + fliD MCAODC_19620 0.70 -1.2
756,675 + fliD MCAODC_19620 0.72 -0.1
756,781 + fliD MCAODC_19620 0.79 +0.4
756,920 + fliD MCAODC_19620 0.89 -0.2
756,921 - fliD MCAODC_19620 0.89 +2.0
757,029 + -0.3
757,034 - -0.7
757,058 + -0.0
757,058 + -1.8
757,098 + +1.2
757,122 + +0.4
757,122 + -0.7
757,359 + fliS MCAODC_19625 0.64 -0.1
757,359 + fliS MCAODC_19625 0.64 -1.0
757,361 + fliS MCAODC_19625 0.65 +0.9
757,361 + fliS MCAODC_19625 0.65 +0.8
757,361 + fliS MCAODC_19625 0.65 -0.9
757,361 + fliS MCAODC_19625 0.65 +0.6
757,361 + fliS MCAODC_19625 0.65 -0.0
757,362 - fliS MCAODC_19625 0.65 -1.0
757,362 - fliS MCAODC_19625 0.65 +0.9
757,365 + fliS MCAODC_19625 0.66 +0.9
757,365 + fliS MCAODC_19625 0.66 -0.7
757,365 + fliS MCAODC_19625 0.66 -0.0
757,365 + fliS MCAODC_19625 0.66 -0.9
757,367 + fliS MCAODC_19625 0.66 -0.1
757,367 + fliS MCAODC_19625 0.66 +1.6
757,435 + fliS MCAODC_19625 0.83 +0.1
757,445 + fliS MCAODC_19625 0.85 -0.3
757,524 + +0.6
757,527 - +1.3
757,569 + fliT MCAODC_19630 0.17 +2.5
757,622 + fliT MCAODC_19630 0.32 +0.7
757,622 + fliT MCAODC_19630 0.32 +0.5
757,622 + fliT MCAODC_19630 0.32 +0.3
757,622 + fliT MCAODC_19630 0.32 -1.5
757,623 - fliT MCAODC_19630 0.32 +0.2
757,737 + fliT MCAODC_19630 0.63 +0.2
757,737 + fliT MCAODC_19630 0.63 +0.7
757,738 - fliT MCAODC_19630 0.64 +0.1
757,920 + -0.3
757,920 + -0.5
757,920 + +1.2
757,920 + +1.2
757,920 + -1.8
757,921 - +2.2
757,954 - +0.4
757,968 - -0.7
757,968 - -1.1
757,968 - -0.5
757,987 + -2.3
758,052 + +0.1
758,080 + +0.8
758,123 - amyA MCAODC_19635 0.12 +3.1
758,129 + amyA MCAODC_19635 0.12 -1.3
758,252 + amyA MCAODC_19635 0.20 +0.2
758,252 + amyA MCAODC_19635 0.20 +0.3
758,289 + amyA MCAODC_19635 0.23 -2.0
758,406 + amyA MCAODC_19635 0.31 +1.1
758,427 + amyA MCAODC_19635 0.32 +1.3
758,473 - amyA MCAODC_19635 0.35 -0.9
758,599 + amyA MCAODC_19635 0.44 +2.2
758,599 + amyA MCAODC_19635 0.44 +1.6
758,599 + amyA MCAODC_19635 0.44 +0.3
758,599 + amyA MCAODC_19635 0.44 +1.0
758,664 + amyA MCAODC_19635 0.48 +0.1
758,680 + amyA MCAODC_19635 0.49 +2.5
758,702 + amyA MCAODC_19635 0.51 -0.4
758,702 + amyA MCAODC_19635 0.51 -0.2
758,730 + amyA MCAODC_19635 0.53 +1.7
758,730 + amyA MCAODC_19635 0.53 -0.5
758,776 + amyA MCAODC_19635 0.56 +0.8
758,777 - amyA MCAODC_19635 0.56 +0.3

Or see this region's nucleotide sequence