Experiment: Sodium-DL-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt sppA and sIMPL are separated by 115 nucleotides sIMPL and ansA are separated by 131 nucleotides ansA and pncA are separated by 10 nucleotides pncA and ydjE are separated by 92 nucleotides
MCAODC_18595: sppA - signal peptide peptidase SppA, at 577,617 to 579,473
sppA
MCAODC_18600: sIMPL - SIMPL domain-containing protein, at 579,589 to 580,299
sIMPL
MCAODC_18605: ansA - asparaginase, at 580,431 to 581,447
ansA
MCAODC_18610: pncA - bifunctional nicotinamidase/pyrazinamidase, at 581,458 to 582,099
pncA
MCAODC_18615: ydjE - Inner membrane metabolite transport protein YdjE, at 582,192 to 583,352
ydjE
Position (kb) 580
581
582 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 579.471 kb on + strand at 579.472 kb on - strand at 579.548 kb on + strand at 579.608 kb on + strand at 579.609 kb on - strand at 579.672 kb on + strand, within sIMPL at 579.697 kb on + strand, within sIMPL at 580.179 kb on + strand, within sIMPL at 580.251 kb on + strand at 580.363 kb on + strand at 580.363 kb on + strand at 580.392 kb on - strand at 580.421 kb on + strand at 580.500 kb on + strand at 580.500 kb on + strand at 580.500 kb on + strand at 580.501 kb on - strand at 580.501 kb on - strand at 580.501 kb on - strand at 580.501 kb on - strand at 580.502 kb on + strand at 580.502 kb on + strand at 580.502 kb on + strand at 580.504 kb on + strand at 580.505 kb on - strand at 580.523 kb on - strand at 580.640 kb on + strand, within ansA at 580.641 kb on - strand, within ansA at 580.641 kb on - strand, within ansA at 580.675 kb on - strand, within ansA at 580.716 kb on + strand, within ansA at 580.801 kb on + strand, within ansA at 580.820 kb on + strand, within ansA at 580.839 kb on + strand, within ansA at 580.839 kb on + strand, within ansA at 580.839 kb on + strand, within ansA at 580.870 kb on + strand, within ansA at 580.870 kb on + strand, within ansA at 580.871 kb on - strand, within ansA at 580.884 kb on - strand, within ansA at 580.983 kb on - strand, within ansA at 581.041 kb on + strand, within ansA at 581.071 kb on + strand, within ansA at 581.154 kb on + strand, within ansA at 581.249 kb on + strand, within ansA at 581.249 kb on + strand, within ansA at 581.249 kb on + strand, within ansA at 581.249 kb on + strand, within ansA at 581.335 kb on + strand, within ansA at 581.335 kb on + strand, within ansA at 581.361 kb on + strand at 581.541 kb on + strand, within pncA at 581.541 kb on + strand, within pncA at 581.541 kb on + strand, within pncA at 581.668 kb on - strand, within pncA at 581.745 kb on - strand, within pncA at 581.816 kb on - strand, within pncA at 581.957 kb on + strand, within pncA at 581.957 kb on + strand, within pncA at 581.964 kb on + strand, within pncA at 582.062 kb on + strand at 582.066 kb on + strand at 582.066 kb on + strand at 582.067 kb on - strand at 582.067 kb on - strand at 582.104 kb on - strand at 582.124 kb on - strand at 582.322 kb on - strand, within ydjE
Per-strain Table
Position Strand Gene LocusTag Fraction Sodium-DL-Lactate remove 579,471 + -0.2 579,472 - +0.1 579,548 + +0.9 579,608 + +0.9 579,609 - -1.7 579,672 + sIMPL MCAODC_18600 0.12 -0.2 579,697 + sIMPL MCAODC_18600 0.15 -0.5 580,179 + sIMPL MCAODC_18600 0.83 -1.2 580,251 + +0.0 580,363 + -1.2 580,363 + +1.6 580,392 - -0.7 580,421 + -0.1 580,500 + -0.3 580,500 + +1.2 580,500 + +0.3 580,501 - +0.0 580,501 - -0.0 580,501 - -0.7 580,501 - -0.5 580,502 + -0.3 580,502 + -0.1 580,502 + -0.4 580,504 + +0.3 580,505 - -3.6 580,523 - +1.0 580,640 + ansA MCAODC_18605 0.21 +0.6 580,641 - ansA MCAODC_18605 0.21 +1.9 580,641 - ansA MCAODC_18605 0.21 +0.3 580,675 - ansA MCAODC_18605 0.24 -1.8 580,716 + ansA MCAODC_18605 0.28 +0.6 580,801 + ansA MCAODC_18605 0.36 -1.2 580,820 + ansA MCAODC_18605 0.38 +2.6 580,839 + ansA MCAODC_18605 0.40 +0.5 580,839 + ansA MCAODC_18605 0.40 -1.2 580,839 + ansA MCAODC_18605 0.40 -0.3 580,870 + ansA MCAODC_18605 0.43 +0.3 580,870 + ansA MCAODC_18605 0.43 +0.1 580,871 - ansA MCAODC_18605 0.43 -1.0 580,884 - ansA MCAODC_18605 0.45 -1.3 580,983 - ansA MCAODC_18605 0.54 -0.9 581,041 + ansA MCAODC_18605 0.60 -0.8 581,071 + ansA MCAODC_18605 0.63 -2.0 581,154 + ansA MCAODC_18605 0.71 -0.7 581,249 + ansA MCAODC_18605 0.80 +0.3 581,249 + ansA MCAODC_18605 0.80 +0.7 581,249 + ansA MCAODC_18605 0.80 -0.3 581,249 + ansA MCAODC_18605 0.80 -0.5 581,335 + ansA MCAODC_18605 0.89 -0.4 581,335 + ansA MCAODC_18605 0.89 -0.6 581,361 + -2.7 581,541 + pncA MCAODC_18610 0.13 -0.3 581,541 + pncA MCAODC_18610 0.13 +1.5 581,541 + pncA MCAODC_18610 0.13 -0.7 581,668 - pncA MCAODC_18610 0.33 +0.2 581,745 - pncA MCAODC_18610 0.45 +0.9 581,816 - pncA MCAODC_18610 0.56 +1.1 581,957 + pncA MCAODC_18610 0.78 -0.2 581,957 + pncA MCAODC_18610 0.78 -0.0 581,964 + pncA MCAODC_18610 0.79 -0.2 582,062 + +0.8 582,066 + +0.7 582,066 + +0.8 582,067 - +2.2 582,067 - -0.7 582,104 - -0.4 582,124 - +0.8 582,322 - ydjE MCAODC_18615 0.11 +0.1
Or see this region's nucleotide sequence