Strain Fitness in Escherichia coli ECRC101 around MCAODC_18255

Experiment: Sodium-DL-Lactate

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntydiS and ydiT overlap by 4 nucleotidesydiT and fadK are separated by 56 nucleotides MCAODC_18250: ydiS - putative electron transfer flavoprotein-quinone oxidoreductase YdiS, at 510,378 to 511,667 ydiS MCAODC_18255: ydiT - Ferredoxin-like protein YdiT, at 511,664 to 511,957 ydiT MCAODC_18260: fadK - medium-chain fatty-acid--CoA ligase, at 512,014 to 513,555 fadK Position (kb) 511 512Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 510.684 kb on + strand, within ydiSat 510.685 kb on - strand, within ydiSat 510.685 kb on - strand, within ydiSat 510.685 kb on - strand, within ydiSat 510.686 kb on + strand, within ydiSat 510.687 kb on - strand, within ydiSat 510.691 kb on + strand, within ydiSat 510.824 kb on + strand, within ydiSat 510.864 kb on - strand, within ydiSat 510.932 kb on + strand, within ydiSat 510.980 kb on + strand, within ydiSat 511.058 kb on + strand, within ydiSat 511.214 kb on + strand, within ydiSat 511.313 kb on + strand, within ydiSat 511.420 kb on - strand, within ydiSat 511.781 kb on - strand, within ydiTat 511.813 kb on + strand, within ydiTat 511.813 kb on + strand, within ydiTat 511.917 kb on - strand, within ydiTat 511.956 kb on - strandat 512.164 kb on + strandat 512.165 kb on - strandat 512.165 kb on - strandat 512.170 kb on + strand, within fadKat 512.170 kb on + strand, within fadKat 512.171 kb on - strand, within fadKat 512.171 kb on - strand, within fadKat 512.171 kb on - strand, within fadKat 512.173 kb on - strand, within fadKat 512.283 kb on + strand, within fadKat 512.284 kb on - strand, within fadKat 512.287 kb on + strand, within fadKat 512.287 kb on + strand, within fadKat 512.287 kb on + strand, within fadKat 512.322 kb on - strand, within fadKat 512.367 kb on + strand, within fadKat 512.371 kb on - strand, within fadKat 512.395 kb on - strand, within fadKat 512.432 kb on + strand, within fadKat 512.465 kb on + strand, within fadKat 512.466 kb on - strand, within fadKat 512.519 kb on + strand, within fadKat 512.643 kb on + strand, within fadKat 512.649 kb on + strand, within fadKat 512.650 kb on - strand, within fadKat 512.672 kb on - strand, within fadKat 512.710 kb on - strand, within fadKat 512.772 kb on - strand, within fadKat 512.895 kb on - strand, within fadK

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Per-strain Table

Position Strand Gene LocusTag Fraction Sodium-DL-Lactate
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510,684 + ydiS MCAODC_18250 0.24 -1.4
510,685 - ydiS MCAODC_18250 0.24 +0.6
510,685 - ydiS MCAODC_18250 0.24 -0.7
510,685 - ydiS MCAODC_18250 0.24 -0.2
510,686 + ydiS MCAODC_18250 0.24 +0.8
510,687 - ydiS MCAODC_18250 0.24 +0.2
510,691 + ydiS MCAODC_18250 0.24 -3.2
510,824 + ydiS MCAODC_18250 0.35 -1.2
510,864 - ydiS MCAODC_18250 0.38 -0.1
510,932 + ydiS MCAODC_18250 0.43 -0.4
510,980 + ydiS MCAODC_18250 0.47 -0.4
511,058 + ydiS MCAODC_18250 0.53 -1.3
511,214 + ydiS MCAODC_18250 0.65 +0.5
511,313 + ydiS MCAODC_18250 0.72 -1.9
511,420 - ydiS MCAODC_18250 0.81 +0.2
511,781 - ydiT MCAODC_18255 0.40 +0.1
511,813 + ydiT MCAODC_18255 0.51 +0.3
511,813 + ydiT MCAODC_18255 0.51 +1.4
511,917 - ydiT MCAODC_18255 0.86 -2.3
511,956 - +1.7
512,164 + -1.0
512,165 - -0.2
512,165 - -0.4
512,170 + fadK MCAODC_18260 0.10 -0.2
512,170 + fadK MCAODC_18260 0.10 -1.7
512,171 - fadK MCAODC_18260 0.10 -0.2
512,171 - fadK MCAODC_18260 0.10 +0.3
512,171 - fadK MCAODC_18260 0.10 +1.0
512,173 - fadK MCAODC_18260 0.10 -3.5
512,283 + fadK MCAODC_18260 0.17 -0.5
512,284 - fadK MCAODC_18260 0.18 +1.8
512,287 + fadK MCAODC_18260 0.18 -1.5
512,287 + fadK MCAODC_18260 0.18 -0.9
512,287 + fadK MCAODC_18260 0.18 +0.2
512,322 - fadK MCAODC_18260 0.20 -1.1
512,367 + fadK MCAODC_18260 0.23 +0.9
512,371 - fadK MCAODC_18260 0.23 +0.3
512,395 - fadK MCAODC_18260 0.25 -1.7
512,432 + fadK MCAODC_18260 0.27 +0.2
512,465 + fadK MCAODC_18260 0.29 -0.6
512,466 - fadK MCAODC_18260 0.29 -1.9
512,519 + fadK MCAODC_18260 0.33 +1.1
512,643 + fadK MCAODC_18260 0.41 +0.3
512,649 + fadK MCAODC_18260 0.41 -0.7
512,650 - fadK MCAODC_18260 0.41 +1.7
512,672 - fadK MCAODC_18260 0.43 +1.5
512,710 - fadK MCAODC_18260 0.45 -0.2
512,772 - fadK MCAODC_18260 0.49 -0.9
512,895 - fadK MCAODC_18260 0.57 +0.8

Or see this region's nucleotide sequence