Strain Fitness in Escherichia coli ECRC101 around MCAODC_16675

Experiment: Sodium-DL-Lactate

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyddA and ydeM are separated by 317 nucleotidesydeM and ydeN are separated by 261 nucleotides MCAODC_16670: yddA - Inner membrane ABC transporter ATP-binding protein YddA, at 236,475 to 238,133 yddA MCAODC_16675: ydeM - anaerobic sulfatase maturase, at 238,451 to 239,608 ydeM MCAODC_16680: ydeN - Uncharacterized sulfatase YdeN, at 239,870 to 241,342 ydeN Position (kb) 238 239 240Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 237.581 kb on + strand, within yddAat 237.582 kb on - strand, within yddAat 237.582 kb on - strand, within yddAat 237.629 kb on + strand, within yddAat 238.325 kb on + strandat 238.536 kb on + strandat 238.612 kb on + strand, within ydeMat 239.473 kb on - strand, within ydeMat 239.658 kb on - strandat 239.719 kb on + strandat 239.754 kb on - strandat 239.841 kb on - strandat 239.861 kb on - strandat 239.909 kb on + strandat 240.100 kb on + strand, within ydeNat 240.101 kb on - strand, within ydeNat 240.241 kb on - strand, within ydeNat 240.254 kb on - strand, within ydeNat 240.326 kb on - strand, within ydeNat 240.453 kb on + strand, within ydeNat 240.514 kb on + strand, within ydeN

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Per-strain Table

Position Strand Gene LocusTag Fraction Sodium-DL-Lactate
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237,581 + yddA MCAODC_16670 0.67 +1.8
237,582 - yddA MCAODC_16670 0.67 +2.3
237,582 - yddA MCAODC_16670 0.67 -3.0
237,629 + yddA MCAODC_16670 0.70 +0.2
238,325 + -0.1
238,536 + -1.9
238,612 + ydeM MCAODC_16675 0.14 -2.7
239,473 - ydeM MCAODC_16675 0.88 -0.6
239,658 - +0.5
239,719 + -1.3
239,754 - +1.0
239,841 - +0.8
239,861 - +0.4
239,909 + -0.1
240,100 + ydeN MCAODC_16680 0.16 +1.8
240,101 - ydeN MCAODC_16680 0.16 -3.6
240,241 - ydeN MCAODC_16680 0.25 +0.5
240,254 - ydeN MCAODC_16680 0.26 +2.3
240,326 - ydeN MCAODC_16680 0.31 +0.5
240,453 + ydeN MCAODC_16680 0.40 -0.4
240,514 + ydeN MCAODC_16680 0.44 -2.4

Or see this region's nucleotide sequence