Experiment: Sodium-DL-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt csiD and ygaQ are separated by 336 nucleotides ygaQ and ygaQ are separated by 6 nucleotides
MCAODC_11445: csiD - carbon starvation induced protein CsiD, at 2,272,809 to 2,273,786
csiD
MCAODC_11450: ygaQ - Putative uncharacterized protein YgaQ, at 2,274,123 to 2,275,130
ygaQ
MCAODC_11455: ygaQ - Putative uncharacterized protein YgaQ, at 2,275,137 to 2,276,375
ygaQ
Position (kb)
2274
2275
2276 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2273.183 kb on - strand, within csiD at 2273.272 kb on + strand, within csiD at 2273.272 kb on + strand, within csiD at 2273.272 kb on + strand, within csiD at 2273.272 kb on + strand, within csiD at 2273.273 kb on - strand, within csiD at 2273.294 kb on - strand, within csiD at 2273.342 kb on - strand, within csiD at 2273.342 kb on - strand, within csiD at 2273.370 kb on + strand, within csiD at 2273.524 kb on - strand, within csiD at 2273.587 kb on - strand, within csiD at 2273.611 kb on + strand, within csiD at 2273.734 kb on + strand at 2273.734 kb on + strand at 2273.802 kb on + strand at 2273.802 kb on + strand at 2273.803 kb on - strand at 2273.811 kb on + strand at 2273.811 kb on + strand at 2273.811 kb on + strand at 2273.812 kb on - strand at 2273.812 kb on - strand at 2273.812 kb on - strand at 2273.812 kb on - strand at 2273.948 kb on + strand at 2273.979 kb on - strand at 2274.111 kb on + strand at 2274.120 kb on - strand at 2274.131 kb on - strand at 2274.459 kb on + strand, within ygaQ at 2274.479 kb on - strand, within ygaQ at 2274.647 kb on + strand, within ygaQ at 2274.647 kb on + strand, within ygaQ at 2274.647 kb on + strand, within ygaQ at 2274.648 kb on - strand, within ygaQ at 2274.649 kb on + strand, within ygaQ at 2274.661 kb on - strand, within ygaQ at 2275.300 kb on - strand, within ygaQ at 2275.310 kb on + strand, within ygaQ at 2275.363 kb on - strand, within ygaQ at 2275.562 kb on - strand, within ygaQ at 2275.641 kb on + strand, within ygaQ at 2275.869 kb on - strand, within ygaQ at 2275.913 kb on + strand, within ygaQ at 2275.928 kb on - strand, within ygaQ at 2276.084 kb on + strand, within ygaQ
Per-strain Table
Position Strand Gene LocusTag Fraction Sodium-DL-Lactate remove 2,273,183 - csiD MCAODC_11445 0.38 +0.1 2,273,272 + csiD MCAODC_11445 0.47 -0.2 2,273,272 + csiD MCAODC_11445 0.47 +0.0 2,273,272 + csiD MCAODC_11445 0.47 +0.6 2,273,272 + csiD MCAODC_11445 0.47 +2.2 2,273,273 - csiD MCAODC_11445 0.47 +0.1 2,273,294 - csiD MCAODC_11445 0.50 +0.5 2,273,342 - csiD MCAODC_11445 0.54 -0.9 2,273,342 - csiD MCAODC_11445 0.54 +1.4 2,273,370 + csiD MCAODC_11445 0.57 +0.8 2,273,524 - csiD MCAODC_11445 0.73 -0.0 2,273,587 - csiD MCAODC_11445 0.80 -0.1 2,273,611 + csiD MCAODC_11445 0.82 -1.8 2,273,734 + +1.7 2,273,734 + +0.6 2,273,802 + +0.2 2,273,802 + -0.4 2,273,803 - +0.2 2,273,811 + -1.7 2,273,811 + -0.7 2,273,811 + -2.3 2,273,812 - -0.5 2,273,812 - +0.1 2,273,812 - +0.7 2,273,812 - +1.8 2,273,948 + +0.8 2,273,979 - -0.9 2,274,111 + -3.5 2,274,120 - -0.4 2,274,131 - -0.7 2,274,459 + ygaQ MCAODC_11450 0.33 -1.0 2,274,479 - ygaQ MCAODC_11450 0.35 -0.7 2,274,647 + ygaQ MCAODC_11450 0.52 +1.8 2,274,647 + ygaQ MCAODC_11450 0.52 -0.1 2,274,647 + ygaQ MCAODC_11450 0.52 -0.4 2,274,648 - ygaQ MCAODC_11450 0.52 +0.1 2,274,649 + ygaQ MCAODC_11450 0.52 +1.4 2,274,661 - ygaQ MCAODC_11450 0.53 +1.2 2,275,300 - ygaQ MCAODC_11455 0.13 -0.9 2,275,310 + ygaQ MCAODC_11455 0.14 +0.4 2,275,363 - ygaQ MCAODC_11455 0.18 +0.3 2,275,562 - ygaQ MCAODC_11455 0.34 +1.0 2,275,641 + ygaQ MCAODC_11455 0.41 -0.9 2,275,869 - ygaQ MCAODC_11455 0.59 -0.5 2,275,913 + ygaQ MCAODC_11455 0.63 +1.5 2,275,928 - ygaQ MCAODC_11455 0.64 -0.3 2,276,084 + ygaQ MCAODC_11455 0.76 +0.4
Or see this region's nucleotide sequence