Experiment: Sodium-DL-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MCAODC_05660 and ilvN are separated by 3 nucleotides ilvN and MCAODC_05670 are separated by 262 nucleotides MCAODC_05670 and MCAODC_05675 are separated by 147 nucleotides MCAODC_05675 and MCAODC_05680 are separated by 277 nucleotides
MCAODC_05660: MCAODC_05660 - Acetolactate synthase, at 1,142,912 to 1,144,600
_05660
MCAODC_05665: ilvN - acetolactate synthase small subunit, at 1,144,604 to 1,144,894
ilvN
MCAODC_05670: MCAODC_05670 - hypothetical protein, at 1,145,157 to 1,145,810
_05670
MCAODC_05675: MCAODC_05675 - hypothetical protein, at 1,145,958 to 1,146,440
_05675
MCAODC_05680: MCAODC_05680 - hypothetical protein, at 1,146,718 to 1,147,032
_05680
Position (kb)
1145
1146 Strain fitness (log2 ratio)
-1
0
1
2
3 at 1144.261 kb on + strand, within MCAODC_05660 at 1144.262 kb on - strand, within MCAODC_05660 at 1144.262 kb on - strand, within MCAODC_05660 at 1144.364 kb on + strand, within MCAODC_05660 at 1144.365 kb on - strand, within MCAODC_05660 at 1144.387 kb on + strand, within MCAODC_05660 at 1144.387 kb on + strand, within MCAODC_05660 at 1144.388 kb on - strand, within MCAODC_05660 at 1144.392 kb on - strand, within MCAODC_05660 at 1144.412 kb on - strand, within MCAODC_05660 at 1144.598 kb on + strand at 1144.666 kb on - strand, within ilvN at 1144.756 kb on + strand, within ilvN at 1144.756 kb on + strand, within ilvN at 1144.757 kb on - strand, within ilvN at 1144.814 kb on - strand, within ilvN at 1144.868 kb on - strand at 1144.924 kb on - strand at 1144.944 kb on - strand at 1144.957 kb on + strand at 1144.957 kb on + strand at 1144.957 kb on + strand at 1144.957 kb on + strand at 1144.958 kb on - strand at 1144.958 kb on - strand at 1144.959 kb on + strand at 1144.959 kb on + strand at 1144.959 kb on + strand at 1144.960 kb on - strand at 1144.961 kb on + strand at 1144.961 kb on + strand at 1145.001 kb on - strand at 1145.101 kb on - strand at 1145.449 kb on - strand, within MCAODC_05670 at 1145.634 kb on - strand, within MCAODC_05670 at 1145.815 kb on + strand at 1146.452 kb on + strand at 1146.464 kb on - strand at 1146.545 kb on - strand at 1146.551 kb on + strand at 1146.555 kb on - strand at 1146.599 kb on + strand at 1146.714 kb on + strand at 1146.718 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Sodium-DL-Lactate remove 1,144,261 + MCAODC_05660 0.80 +0.0 1,144,262 - MCAODC_05660 0.80 +0.4 1,144,262 - MCAODC_05660 0.80 -0.7 1,144,364 + MCAODC_05660 0.86 -0.0 1,144,365 - MCAODC_05660 0.86 -0.6 1,144,387 + MCAODC_05660 0.87 +0.4 1,144,387 + MCAODC_05660 0.87 -0.4 1,144,388 - MCAODC_05660 0.87 +0.5 1,144,392 - MCAODC_05660 0.88 -1.7 1,144,412 - MCAODC_05660 0.89 -0.1 1,144,598 + +0.1 1,144,666 - ilvN MCAODC_05665 0.21 +1.0 1,144,756 + ilvN MCAODC_05665 0.52 +0.6 1,144,756 + ilvN MCAODC_05665 0.52 +0.7 1,144,757 - ilvN MCAODC_05665 0.53 +1.1 1,144,814 - ilvN MCAODC_05665 0.72 -1.5 1,144,868 - +0.2 1,144,924 - +0.9 1,144,944 - -0.0 1,144,957 + +0.8 1,144,957 + +0.7 1,144,957 + -1.3 1,144,957 + -0.4 1,144,958 - +0.4 1,144,958 - -0.2 1,144,959 + -0.1 1,144,959 + -1.7 1,144,959 + +0.6 1,144,960 - +1.3 1,144,961 + +0.2 1,144,961 + +0.5 1,145,001 - -0.3 1,145,101 - +0.0 1,145,449 - MCAODC_05670 0.45 -1.5 1,145,634 - MCAODC_05670 0.73 +0.2 1,145,815 + -0.6 1,146,452 + +0.1 1,146,464 - -0.4 1,146,545 - +1.2 1,146,551 + +2.8 1,146,555 - +0.9 1,146,599 + -1.3 1,146,714 + -0.7 1,146,718 - +1.0
Or see this region's nucleotide sequence