Strain Fitness in Escherichia coli ECRC101 around MCAODC_05665

Experiment: Sodium-DL-Lactate

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntMCAODC_05660 and ilvN are separated by 3 nucleotidesilvN and MCAODC_05670 are separated by 262 nucleotides MCAODC_05660: MCAODC_05660 - Acetolactate synthase, at 1,142,912 to 1,144,600 _05660 MCAODC_05665: ilvN - acetolactate synthase small subunit, at 1,144,604 to 1,144,894 ilvN MCAODC_05670: MCAODC_05670 - hypothetical protein, at 1,145,157 to 1,145,810 _05670 Position (kb) 1144 1145Strain fitness (log2 ratio) -2 -1 0 1at 1143.643 kb on - strand, within MCAODC_05660at 1143.688 kb on + strand, within MCAODC_05660at 1143.689 kb on - strand, within MCAODC_05660at 1143.689 kb on - strand, within MCAODC_05660at 1143.689 kb on - strand, within MCAODC_05660at 1143.722 kb on + strand, within MCAODC_05660at 1143.722 kb on + strand, within MCAODC_05660at 1143.723 kb on - strand, within MCAODC_05660at 1143.724 kb on + strand, within MCAODC_05660at 1143.821 kb on - strand, within MCAODC_05660at 1143.821 kb on - strand, within MCAODC_05660at 1143.873 kb on + strand, within MCAODC_05660at 1143.874 kb on - strand, within MCAODC_05660at 1144.014 kb on + strand, within MCAODC_05660at 1144.022 kb on + strand, within MCAODC_05660at 1144.070 kb on - strand, within MCAODC_05660at 1144.261 kb on + strand, within MCAODC_05660at 1144.262 kb on - strand, within MCAODC_05660at 1144.262 kb on - strand, within MCAODC_05660at 1144.364 kb on + strand, within MCAODC_05660at 1144.365 kb on - strand, within MCAODC_05660at 1144.387 kb on + strand, within MCAODC_05660at 1144.387 kb on + strand, within MCAODC_05660at 1144.388 kb on - strand, within MCAODC_05660at 1144.392 kb on - strand, within MCAODC_05660at 1144.412 kb on - strand, within MCAODC_05660at 1144.598 kb on + strandat 1144.666 kb on - strand, within ilvNat 1144.756 kb on + strand, within ilvNat 1144.756 kb on + strand, within ilvNat 1144.757 kb on - strand, within ilvNat 1144.814 kb on - strand, within ilvNat 1144.868 kb on - strandat 1144.924 kb on - strandat 1144.944 kb on - strandat 1144.957 kb on + strandat 1144.957 kb on + strandat 1144.957 kb on + strandat 1144.957 kb on + strandat 1144.958 kb on - strandat 1144.958 kb on - strandat 1144.959 kb on + strandat 1144.959 kb on + strandat 1144.959 kb on + strandat 1144.960 kb on - strandat 1144.961 kb on + strandat 1144.961 kb on + strandat 1145.001 kb on - strandat 1145.101 kb on - strandat 1145.449 kb on - strand, within MCAODC_05670at 1145.634 kb on - strand, within MCAODC_05670at 1145.815 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Sodium-DL-Lactate
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1,143,643 - MCAODC_05660 0.43 -0.7
1,143,688 + MCAODC_05660 0.46 +0.4
1,143,689 - MCAODC_05660 0.46 +0.4
1,143,689 - MCAODC_05660 0.46 -1.9
1,143,689 - MCAODC_05660 0.46 -1.0
1,143,722 + MCAODC_05660 0.48 -0.2
1,143,722 + MCAODC_05660 0.48 -0.1
1,143,723 - MCAODC_05660 0.48 +0.5
1,143,724 + MCAODC_05660 0.48 -0.2
1,143,821 - MCAODC_05660 0.54 +0.2
1,143,821 - MCAODC_05660 0.54 -0.2
1,143,873 + MCAODC_05660 0.57 +0.3
1,143,874 - MCAODC_05660 0.57 +0.2
1,144,014 + MCAODC_05660 0.65 +0.2
1,144,022 + MCAODC_05660 0.66 +0.3
1,144,070 - MCAODC_05660 0.69 -0.6
1,144,261 + MCAODC_05660 0.80 +0.0
1,144,262 - MCAODC_05660 0.80 +0.4
1,144,262 - MCAODC_05660 0.80 -0.7
1,144,364 + MCAODC_05660 0.86 -0.0
1,144,365 - MCAODC_05660 0.86 -0.6
1,144,387 + MCAODC_05660 0.87 +0.4
1,144,387 + MCAODC_05660 0.87 -0.4
1,144,388 - MCAODC_05660 0.87 +0.5
1,144,392 - MCAODC_05660 0.88 -1.7
1,144,412 - MCAODC_05660 0.89 -0.1
1,144,598 + +0.1
1,144,666 - ilvN MCAODC_05665 0.21 +1.0
1,144,756 + ilvN MCAODC_05665 0.52 +0.6
1,144,756 + ilvN MCAODC_05665 0.52 +0.7
1,144,757 - ilvN MCAODC_05665 0.53 +1.1
1,144,814 - ilvN MCAODC_05665 0.72 -1.5
1,144,868 - +0.2
1,144,924 - +0.9
1,144,944 - -0.0
1,144,957 + +0.8
1,144,957 + +0.7
1,144,957 + -1.3
1,144,957 + -0.4
1,144,958 - +0.4
1,144,958 - -0.2
1,144,959 + -0.1
1,144,959 + -1.7
1,144,959 + +0.6
1,144,960 - +1.3
1,144,961 + +0.2
1,144,961 + +0.5
1,145,001 - -0.3
1,145,101 - +0.0
1,145,449 - MCAODC_05670 0.45 -1.5
1,145,634 - MCAODC_05670 0.73 +0.2
1,145,815 + -0.6

Or see this region's nucleotide sequence