Strain Fitness in Escherichia coli ECRC101 around MCAODC_03425

Experiment: Sodium-DL-Lactate

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntaphA and MCAODC_03425 are separated by 400 nucleotidesMCAODC_03425 and tyrB are separated by 338 nucleotides MCAODC_03420: aphA - acid phosphatase AphA, at 690,791 to 691,504 aphA MCAODC_03425: MCAODC_03425 - hypothetical protein, at 691,905 to 692,264 _03425 MCAODC_03430: tyrB - aromatic amino acid transaminase, at 692,603 to 693,796 tyrB Position (kb) 691 692 693Strain fitness (log2 ratio) -2 -1 0 1 2at 690.917 kb on - strand, within aphAat 690.917 kb on - strand, within aphAat 691.124 kb on + strand, within aphAat 691.124 kb on + strand, within aphAat 691.125 kb on - strand, within aphAat 691.125 kb on - strand, within aphAat 691.125 kb on - strand, within aphAat 691.125 kb on - strand, within aphAat 691.125 kb on - strand, within aphAat 691.125 kb on - strand, within aphAat 691.132 kb on + strand, within aphAat 691.132 kb on + strand, within aphAat 691.133 kb on - strand, within aphAat 691.205 kb on + strand, within aphAat 691.221 kb on + strand, within aphAat 691.221 kb on + strand, within aphAat 691.222 kb on - strand, within aphAat 691.283 kb on + strand, within aphAat 691.399 kb on + strand, within aphAat 691.519 kb on - strandat 691.525 kb on + strandat 691.604 kb on - strandat 691.639 kb on + strandat 691.648 kb on + strandat 691.699 kb on + strandat 691.705 kb on + strandat 691.863 kb on - strandat 691.875 kb on - strandat 691.946 kb on - strand, within MCAODC_03425at 692.010 kb on - strand, within MCAODC_03425at 692.107 kb on + strand, within MCAODC_03425at 692.138 kb on + strand, within MCAODC_03425at 692.142 kb on + strand, within MCAODC_03425at 692.197 kb on - strand, within MCAODC_03425at 692.531 kb on - strandat 692.605 kb on - strandat 692.605 kb on - strandat 692.650 kb on + strandat 692.651 kb on - strandat 692.691 kb on + strandat 692.692 kb on - strandat 692.712 kb on - strandat 692.712 kb on - strandat 692.735 kb on + strand, within tyrBat 692.743 kb on + strand, within tyrBat 692.744 kb on - strand, within tyrBat 692.745 kb on + strand, within tyrBat 692.745 kb on + strand, within tyrBat 692.746 kb on - strand, within tyrBat 692.767 kb on + strand, within tyrBat 692.807 kb on + strand, within tyrBat 692.808 kb on - strand, within tyrBat 692.808 kb on - strand, within tyrBat 692.810 kb on + strand, within tyrBat 692.810 kb on + strand, within tyrBat 692.810 kb on + strand, within tyrBat 692.849 kb on - strand, within tyrBat 692.861 kb on + strand, within tyrBat 692.946 kb on + strand, within tyrBat 692.946 kb on + strand, within tyrBat 693.153 kb on + strand, within tyrBat 693.153 kb on + strand, within tyrBat 693.154 kb on - strand, within tyrBat 693.161 kb on - strand, within tyrB

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Per-strain Table

Position Strand Gene LocusTag Fraction Sodium-DL-Lactate
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690,917 - aphA MCAODC_03420 0.18 -0.0
690,917 - aphA MCAODC_03420 0.18 -1.8
691,124 + aphA MCAODC_03420 0.47 +0.7
691,124 + aphA MCAODC_03420 0.47 +0.1
691,125 - aphA MCAODC_03420 0.47 -0.6
691,125 - aphA MCAODC_03420 0.47 +0.2
691,125 - aphA MCAODC_03420 0.47 -0.3
691,125 - aphA MCAODC_03420 0.47 +1.0
691,125 - aphA MCAODC_03420 0.47 +0.8
691,125 - aphA MCAODC_03420 0.47 +0.1
691,132 + aphA MCAODC_03420 0.48 -0.6
691,132 + aphA MCAODC_03420 0.48 +0.7
691,133 - aphA MCAODC_03420 0.48 +0.4
691,205 + aphA MCAODC_03420 0.58 +0.9
691,221 + aphA MCAODC_03420 0.60 -1.8
691,221 + aphA MCAODC_03420 0.60 -0.0
691,222 - aphA MCAODC_03420 0.60 -1.6
691,283 + aphA MCAODC_03420 0.69 +1.2
691,399 + aphA MCAODC_03420 0.85 -0.6
691,519 - -0.5
691,525 + -0.8
691,604 - -0.0
691,639 + +0.2
691,648 + +0.1
691,699 + +1.8
691,705 + +1.2
691,863 - +1.3
691,875 - -0.9
691,946 - MCAODC_03425 0.11 +0.6
692,010 - MCAODC_03425 0.29 +1.1
692,107 + MCAODC_03425 0.56 +1.8
692,138 + MCAODC_03425 0.65 +0.4
692,142 + MCAODC_03425 0.66 +0.2
692,197 - MCAODC_03425 0.81 +0.8
692,531 - -0.3
692,605 - -0.2
692,605 - +0.6
692,650 + -0.4
692,651 - -0.2
692,691 + +0.8
692,692 - -0.3
692,712 - +0.3
692,712 - -0.0
692,735 + tyrB MCAODC_03430 0.11 -0.3
692,743 + tyrB MCAODC_03430 0.12 +1.1
692,744 - tyrB MCAODC_03430 0.12 -1.1
692,745 + tyrB MCAODC_03430 0.12 -0.1
692,745 + tyrB MCAODC_03430 0.12 -0.3
692,746 - tyrB MCAODC_03430 0.12 -0.2
692,767 + tyrB MCAODC_03430 0.14 -0.5
692,807 + tyrB MCAODC_03430 0.17 +0.8
692,808 - tyrB MCAODC_03430 0.17 +0.1
692,808 - tyrB MCAODC_03430 0.17 -0.2
692,810 + tyrB MCAODC_03430 0.17 +0.0
692,810 + tyrB MCAODC_03430 0.17 -0.1
692,810 + tyrB MCAODC_03430 0.17 +0.2
692,849 - tyrB MCAODC_03430 0.21 -0.8
692,861 + tyrB MCAODC_03430 0.22 -0.2
692,946 + tyrB MCAODC_03430 0.29 +0.3
692,946 + tyrB MCAODC_03430 0.29 +0.1
693,153 + tyrB MCAODC_03430 0.46 +1.5
693,153 + tyrB MCAODC_03430 0.46 +0.1
693,154 - tyrB MCAODC_03430 0.46 -0.4
693,161 - tyrB MCAODC_03430 0.47 +0.7

Or see this region's nucleotide sequence