Experiment: Sodium-DL-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rbsA and MCAODC_03225 overlap by 4 nucleotides MCAODC_03225 and rbsB are separated by 18 nucleotides rbsB and ydaE are separated by 54 nucleotides ydaE and fba are separated by 14 nucleotides
MCAODC_03220: rbsA - Ribose import ATP-binding protein RbsA, at 647,726 to 649,246
rbsA
MCAODC_03225: MCAODC_03225 - ribose ABC transporter permease, at 649,243 to 650,217
_03225
MCAODC_03230: rbsB - transcriptional regulator, at 650,236 to 651,177
rbsB
MCAODC_03235: ydaE - D-lyxose/D-mannose isomerase, at 651,232 to 651,915
ydaE
MCAODC_03240: fba - ketose 1,6-bisphosphate aldolase, at 651,930 to 652,790
fba
Position (kb) 650
651
652 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 649.389 kb on + strand, within MCAODC_03225 at 649.522 kb on - strand, within MCAODC_03225 at 649.645 kb on + strand, within MCAODC_03225 at 649.645 kb on + strand, within MCAODC_03225 at 649.646 kb on - strand, within MCAODC_03225 at 649.646 kb on - strand, within MCAODC_03225 at 649.646 kb on - strand, within MCAODC_03225 at 649.648 kb on - strand, within MCAODC_03225 at 649.648 kb on - strand, within MCAODC_03225 at 649.695 kb on + strand, within MCAODC_03225 at 649.710 kb on + strand, within MCAODC_03225 at 649.735 kb on + strand, within MCAODC_03225 at 649.735 kb on + strand, within MCAODC_03225 at 649.735 kb on + strand, within MCAODC_03225 at 649.736 kb on - strand, within MCAODC_03225 at 649.736 kb on - strand, within MCAODC_03225 at 649.736 kb on - strand, within MCAODC_03225 at 649.736 kb on - strand, within MCAODC_03225 at 649.736 kb on - strand, within MCAODC_03225 at 649.736 kb on - strand, within MCAODC_03225 at 649.736 kb on - strand, within MCAODC_03225 at 649.827 kb on + strand, within MCAODC_03225 at 649.827 kb on + strand, within MCAODC_03225 at 649.828 kb on - strand, within MCAODC_03225 at 649.828 kb on - strand, within MCAODC_03225 at 649.828 kb on - strand, within MCAODC_03225 at 649.831 kb on + strand, within MCAODC_03225 at 649.831 kb on + strand, within MCAODC_03225 at 649.867 kb on - strand, within MCAODC_03225 at 649.867 kb on - strand, within MCAODC_03225 at 649.875 kb on + strand, within MCAODC_03225 at 649.875 kb on + strand, within MCAODC_03225 at 649.876 kb on - strand, within MCAODC_03225 at 649.904 kb on - strand, within MCAODC_03225 at 649.960 kb on + strand, within MCAODC_03225 at 649.979 kb on + strand, within MCAODC_03225 at 649.979 kb on + strand, within MCAODC_03225 at 649.980 kb on - strand, within MCAODC_03225 at 649.980 kb on - strand, within MCAODC_03225 at 649.980 kb on - strand, within MCAODC_03225 at 649.980 kb on - strand, within MCAODC_03225 at 650.081 kb on + strand, within MCAODC_03225 at 650.085 kb on + strand, within MCAODC_03225 at 650.128 kb on + strand at 650.128 kb on + strand at 650.128 kb on + strand at 650.129 kb on - strand at 650.129 kb on - strand at 650.189 kb on - strand at 650.230 kb on + strand at 650.231 kb on - strand at 650.231 kb on - strand at 650.331 kb on - strand, within rbsB at 650.444 kb on - strand, within rbsB at 650.471 kb on + strand, within rbsB at 650.472 kb on - strand, within rbsB at 650.472 kb on - strand, within rbsB at 650.505 kb on + strand, within rbsB at 650.592 kb on - strand, within rbsB at 650.655 kb on + strand, within rbsB at 650.655 kb on + strand, within rbsB at 650.656 kb on - strand, within rbsB at 650.656 kb on - strand, within rbsB at 650.659 kb on + strand, within rbsB at 650.659 kb on + strand, within rbsB at 650.659 kb on + strand, within rbsB at 650.659 kb on + strand, within rbsB at 650.659 kb on + strand, within rbsB at 650.659 kb on + strand, within rbsB at 650.659 kb on + strand, within rbsB at 650.660 kb on - strand, within rbsB at 650.660 kb on - strand, within rbsB at 650.660 kb on - strand, within rbsB at 650.660 kb on - strand, within rbsB at 650.660 kb on - strand, within rbsB at 650.660 kb on - strand, within rbsB at 650.660 kb on - strand, within rbsB at 650.756 kb on - strand, within rbsB at 650.834 kb on + strand, within rbsB at 650.834 kb on + strand, within rbsB at 650.834 kb on + strand, within rbsB at 650.835 kb on - strand, within rbsB at 650.876 kb on + strand, within rbsB at 650.876 kb on + strand, within rbsB at 650.938 kb on - strand, within rbsB at 650.949 kb on + strand, within rbsB at 651.154 kb on + strand at 651.155 kb on - strand at 651.155 kb on - strand at 651.175 kb on + strand at 651.175 kb on + strand at 651.176 kb on - strand at 651.246 kb on + strand at 651.247 kb on - strand at 651.248 kb on + strand at 651.248 kb on + strand at 651.267 kb on + strand at 651.267 kb on + strand at 651.268 kb on - strand at 651.296 kb on + strand at 651.296 kb on + strand at 651.297 kb on - strand at 651.297 kb on - strand at 651.297 kb on - strand at 651.444 kb on + strand, within ydaE at 651.478 kb on + strand, within ydaE at 651.479 kb on - strand, within ydaE at 651.479 kb on - strand, within ydaE at 651.479 kb on - strand, within ydaE at 651.552 kb on + strand, within ydaE at 651.552 kb on + strand, within ydaE at 651.585 kb on + strand, within ydaE at 651.586 kb on - strand, within ydaE at 651.591 kb on - strand, within ydaE at 651.591 kb on - strand, within ydaE at 651.618 kb on + strand, within ydaE at 651.618 kb on + strand, within ydaE at 651.619 kb on - strand, within ydaE at 651.656 kb on + strand, within ydaE at 651.656 kb on + strand, within ydaE at 651.656 kb on + strand, within ydaE at 651.656 kb on + strand, within ydaE at 651.657 kb on - strand, within ydaE at 651.678 kb on + strand, within ydaE at 651.679 kb on - strand, within ydaE at 651.679 kb on - strand, within ydaE at 651.679 kb on - strand, within ydaE at 651.717 kb on + strand, within ydaE at 651.717 kb on + strand, within ydaE at 651.718 kb on - strand, within ydaE at 651.739 kb on + strand, within ydaE at 651.740 kb on - strand, within ydaE at 651.848 kb on - strand at 651.884 kb on + strand at 651.885 kb on - strand at 651.930 kb on - strand at 651.982 kb on - strand at 652.064 kb on + strand, within fba at 652.064 kb on + strand, within fba at 652.084 kb on + strand, within fba at 652.085 kb on - strand, within fba at 652.085 kb on - strand, within fba at 652.091 kb on + strand, within fba at 652.095 kb on + strand, within fba at 652.095 kb on + strand, within fba at 652.097 kb on + strand, within fba at 652.097 kb on + strand, within fba at 652.165 kb on + strand, within fba at 652.166 kb on - strand, within fba
Per-strain Table
Position Strand Gene LocusTag Fraction Sodium-DL-Lactate remove 649,389 + MCAODC_03225 0.15 -0.2 649,522 - MCAODC_03225 0.29 +1.9 649,645 + MCAODC_03225 0.41 -0.9 649,645 + MCAODC_03225 0.41 +0.1 649,646 - MCAODC_03225 0.41 +0.3 649,646 - MCAODC_03225 0.41 +0.0 649,646 - MCAODC_03225 0.41 +1.2 649,648 - MCAODC_03225 0.42 +0.0 649,648 - MCAODC_03225 0.42 -1.2 649,695 + MCAODC_03225 0.46 -1.6 649,710 + MCAODC_03225 0.48 +0.3 649,735 + MCAODC_03225 0.50 -3.5 649,735 + MCAODC_03225 0.50 +0.8 649,735 + MCAODC_03225 0.50 +1.5 649,736 - MCAODC_03225 0.51 -1.7 649,736 - MCAODC_03225 0.51 +0.1 649,736 - MCAODC_03225 0.51 +0.4 649,736 - MCAODC_03225 0.51 +0.2 649,736 - MCAODC_03225 0.51 +0.5 649,736 - MCAODC_03225 0.51 +1.4 649,736 - MCAODC_03225 0.51 +0.2 649,827 + MCAODC_03225 0.60 +1.6 649,827 + MCAODC_03225 0.60 -0.1 649,828 - MCAODC_03225 0.60 +0.5 649,828 - MCAODC_03225 0.60 +0.2 649,828 - MCAODC_03225 0.60 -1.5 649,831 + MCAODC_03225 0.60 -1.7 649,831 + MCAODC_03225 0.60 +0.5 649,867 - MCAODC_03225 0.64 -0.4 649,867 - MCAODC_03225 0.64 +0.3 649,875 + MCAODC_03225 0.65 -0.3 649,875 + MCAODC_03225 0.65 +0.7 649,876 - MCAODC_03225 0.65 -0.7 649,904 - MCAODC_03225 0.68 -1.9 649,960 + MCAODC_03225 0.74 -1.9 649,979 + MCAODC_03225 0.75 +0.6 649,979 + MCAODC_03225 0.75 +0.6 649,980 - MCAODC_03225 0.76 +0.2 649,980 - MCAODC_03225 0.76 +0.3 649,980 - MCAODC_03225 0.76 +0.2 649,980 - MCAODC_03225 0.76 +0.3 650,081 + MCAODC_03225 0.86 -0.0 650,085 + MCAODC_03225 0.86 -1.5 650,128 + +0.5 650,128 + +0.5 650,128 + -0.1 650,129 - -0.0 650,129 - +1.2 650,189 - +0.8 650,230 + +1.6 650,231 - -0.1 650,231 - -0.5 650,331 - rbsB MCAODC_03230 0.10 -1.0 650,444 - rbsB MCAODC_03230 0.22 -0.8 650,471 + rbsB MCAODC_03230 0.25 +0.7 650,472 - rbsB MCAODC_03230 0.25 -2.7 650,472 - rbsB MCAODC_03230 0.25 +1.0 650,505 + rbsB MCAODC_03230 0.29 +0.2 650,592 - rbsB MCAODC_03230 0.38 +0.6 650,655 + rbsB MCAODC_03230 0.44 +0.2 650,655 + rbsB MCAODC_03230 0.44 -0.7 650,656 - rbsB MCAODC_03230 0.45 +0.8 650,656 - rbsB MCAODC_03230 0.45 +0.1 650,659 + rbsB MCAODC_03230 0.45 -0.4 650,659 + rbsB MCAODC_03230 0.45 -0.4 650,659 + rbsB MCAODC_03230 0.45 +0.8 650,659 + rbsB MCAODC_03230 0.45 +0.4 650,659 + rbsB MCAODC_03230 0.45 -1.3 650,659 + rbsB MCAODC_03230 0.45 -0.1 650,659 + rbsB MCAODC_03230 0.45 +1.3 650,660 - rbsB MCAODC_03230 0.45 +0.7 650,660 - rbsB MCAODC_03230 0.45 +1.0 650,660 - rbsB MCAODC_03230 0.45 -1.0 650,660 - rbsB MCAODC_03230 0.45 +0.6 650,660 - rbsB MCAODC_03230 0.45 +0.4 650,660 - rbsB MCAODC_03230 0.45 -0.5 650,660 - rbsB MCAODC_03230 0.45 -0.7 650,756 - rbsB MCAODC_03230 0.55 -0.3 650,834 + rbsB MCAODC_03230 0.63 -0.2 650,834 + rbsB MCAODC_03230 0.63 +1.5 650,834 + rbsB MCAODC_03230 0.63 -1.8 650,835 - rbsB MCAODC_03230 0.64 -0.3 650,876 + rbsB MCAODC_03230 0.68 -0.1 650,876 + rbsB MCAODC_03230 0.68 +0.7 650,938 - rbsB MCAODC_03230 0.75 +0.3 650,949 + rbsB MCAODC_03230 0.76 +0.3 651,154 + +0.9 651,155 - +0.0 651,155 - -0.4 651,175 + +0.5 651,175 + -0.1 651,176 - -1.6 651,246 + +0.5 651,247 - +0.1 651,248 + -0.6 651,248 + +0.2 651,267 + +0.4 651,267 + -0.8 651,268 - +0.4 651,296 + -0.4 651,296 + -0.8 651,297 - +0.5 651,297 - -0.4 651,297 - -0.2 651,444 + ydaE MCAODC_03235 0.31 -0.1 651,478 + ydaE MCAODC_03235 0.36 -0.5 651,479 - ydaE MCAODC_03235 0.36 -0.3 651,479 - ydaE MCAODC_03235 0.36 -0.1 651,479 - ydaE MCAODC_03235 0.36 +1.1 651,552 + ydaE MCAODC_03235 0.47 -0.0 651,552 + ydaE MCAODC_03235 0.47 -0.5 651,585 + ydaE MCAODC_03235 0.52 +0.9 651,586 - ydaE MCAODC_03235 0.52 -0.9 651,591 - ydaE MCAODC_03235 0.52 +0.1 651,591 - ydaE MCAODC_03235 0.52 -0.0 651,618 + ydaE MCAODC_03235 0.56 -0.3 651,618 + ydaE MCAODC_03235 0.56 -1.7 651,619 - ydaE MCAODC_03235 0.57 -0.6 651,656 + ydaE MCAODC_03235 0.62 -0.3 651,656 + ydaE MCAODC_03235 0.62 +1.8 651,656 + ydaE MCAODC_03235 0.62 +1.2 651,656 + ydaE MCAODC_03235 0.62 +0.5 651,657 - ydaE MCAODC_03235 0.62 +0.3 651,678 + ydaE MCAODC_03235 0.65 -0.3 651,679 - ydaE MCAODC_03235 0.65 -0.7 651,679 - ydaE MCAODC_03235 0.65 +0.0 651,679 - ydaE MCAODC_03235 0.65 -1.0 651,717 + ydaE MCAODC_03235 0.71 +0.9 651,717 + ydaE MCAODC_03235 0.71 +0.3 651,718 - ydaE MCAODC_03235 0.71 -1.0 651,739 + ydaE MCAODC_03235 0.74 -0.8 651,740 - ydaE MCAODC_03235 0.74 -0.3 651,848 - +0.1 651,884 + -0.3 651,885 - -0.8 651,930 - +0.9 651,982 - +0.4 652,064 + fba MCAODC_03240 0.16 +0.4 652,064 + fba MCAODC_03240 0.16 -0.7 652,084 + fba MCAODC_03240 0.18 -1.9 652,085 - fba MCAODC_03240 0.18 +0.3 652,085 - fba MCAODC_03240 0.18 +1.7 652,091 + fba MCAODC_03240 0.19 +0.4 652,095 + fba MCAODC_03240 0.19 -0.9 652,095 + fba MCAODC_03240 0.19 +0.0 652,097 + fba MCAODC_03240 0.19 -2.0 652,097 + fba MCAODC_03240 0.19 -1.2 652,165 + fba MCAODC_03240 0.27 +1.7 652,166 - fba MCAODC_03240 0.27 -0.1
Or see this region's nucleotide sequence