Experiment: Bas51
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yibN and grxC are separated by 141 nucleotides grxC and secB are separated by 62 nucleotides secB and gpsA overlap by 1 nucleotides gpsA and cysE are separated by 79 nucleotides cysE and trmL are separated by 52 nucleotides
HEPCGN_14580: yibN - Uncharacterized protein YibN, at 1,671,163 to 1,671,594
yibN
HEPCGN_14585: grxC - glutaredoxin 3, at 1,671,736 to 1,671,987
grxC
HEPCGN_14590: secB - protein-export chaperone SecB, at 1,672,050 to 1,672,517
secB
HEPCGN_14595: gpsA - NAD(P)H-dependent glycerol-3-phosphate dehydrogenase, at 1,672,517 to 1,673,536
gpsA
HEPCGN_14600: cysE - serine O-acetyltransferase, at 1,673,616 to 1,674,437
cysE
HEPCGN_14605: trmL - tRNA (uridine(34)/cytosine(34)/5-carboxymethylaminomethyluridine (34)-2'-O)-methyltransferase TrmL, at 1,674,490 to 1,674,963
trmL
Position (kb)
1672
1673
1674 Strain fitness (log2 ratio)
-2
-1
0
1 at 1671.527 kb on + strand, within yibN at 1671.598 kb on - strand at 1671.652 kb on + strand at 1671.652 kb on + strand at 1671.652 kb on + strand at 1671.652 kb on + strand at 1671.653 kb on - strand at 1671.653 kb on - strand at 1671.653 kb on - strand at 1671.658 kb on + strand at 1671.660 kb on + strand at 1671.660 kb on + strand at 1671.660 kb on + strand at 1671.661 kb on - strand at 1671.661 kb on - strand at 1671.730 kb on + strand at 1671.730 kb on + strand at 1671.730 kb on + strand at 1671.731 kb on - strand at 1672.017 kb on + strand at 1672.034 kb on + strand at 1672.467 kb on + strand, within secB at 1673.593 kb on + strand at 1673.655 kb on - strand at 1673.786 kb on + strand, within cysE at 1673.923 kb on + strand, within cysE at 1674.366 kb on - strand at 1674.402 kb on - strand at 1674.402 kb on - strand at 1674.405 kb on - strand at 1674.410 kb on + strand at 1674.410 kb on + strand at 1674.411 kb on - strand at 1674.465 kb on + strand at 1674.465 kb on + strand at 1674.465 kb on + strand at 1674.466 kb on - strand at 1674.466 kb on - strand at 1674.466 kb on - strand at 1674.482 kb on + strand at 1674.482 kb on + strand at 1674.487 kb on + strand at 1674.487 kb on + strand at 1674.492 kb on - strand at 1674.492 kb on - strand at 1674.492 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bas51 remove 1,671,527 + yibN HEPCGN_14580 0.84 -0.9 1,671,598 - +0.7 1,671,652 + +0.5 1,671,652 + +1.1 1,671,652 + +0.2 1,671,652 + +0.4 1,671,653 - +0.1 1,671,653 - +0.7 1,671,653 - +0.1 1,671,658 + +0.5 1,671,660 + -0.0 1,671,660 + -0.0 1,671,660 + -0.2 1,671,661 - +0.4 1,671,661 - -2.2 1,671,730 + -0.0 1,671,730 + +0.5 1,671,730 + +1.0 1,671,731 - -1.7 1,672,017 + -0.0 1,672,034 + -0.8 1,672,467 + secB HEPCGN_14590 0.89 -1.1 1,673,593 + +0.2 1,673,655 - -1.6 1,673,786 + cysE HEPCGN_14600 0.21 -0.5 1,673,923 + cysE HEPCGN_14600 0.37 -0.9 1,674,366 - -0.6 1,674,402 - +0.0 1,674,402 - +0.5 1,674,405 - -1.1 1,674,410 + +0.2 1,674,410 + -1.0 1,674,411 - -0.7 1,674,465 + -0.5 1,674,465 + -0.1 1,674,465 + +0.3 1,674,466 - +0.2 1,674,466 - -0.2 1,674,466 - -0.3 1,674,482 + -0.4 1,674,482 + +0.0 1,674,487 + -0.5 1,674,487 + +0.2 1,674,492 - +0.5 1,674,492 - +0.6 1,674,492 - -0.4
Or see this region's nucleotide sequence