Experiment: Bas51
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ribD and nrdR are separated by 3 nucleotides nrdR and HEPCGN_07090 are separated by 150 nucleotides HEPCGN_07090 and HEPCGN_07095 overlap by 16 nucleotides HEPCGN_07095 and HEPCGN_07100 are separated by 152 nucleotides
HEPCGN_07080: ribD - bifunctional diaminohydroxyphosphoribosylaminopyrimidine deaminase/5-amino-6-(5-phosphoribosylamino)uracil reductase RibD, at 150,828 to 151,931
ribD
HEPCGN_07085: nrdR - transcriptional regulator NrdR, at 151,935 to 152,384
nrdR
HEPCGN_07090: HEPCGN_07090 - hypothetical protein, at 152,535 to 152,723
_07090
HEPCGN_07095: HEPCGN_07095 - RNA-guided endonuclease TnpB family protein, at 152,708 to 153,514
_07095
HEPCGN_07100: HEPCGN_07100 - Putative virulence protein, at 153,667 to 153,855
_07100
Position (kb)
152
153
154 Strain fitness (log2 ratio)
-2
-1
0
1 at 151.977 kb on - strand at 152.015 kb on - strand, within nrdR at 152.025 kb on - strand, within nrdR at 152.188 kb on - strand, within nrdR at 152.283 kb on - strand, within nrdR at 152.283 kb on - strand, within nrdR at 152.352 kb on - strand at 152.438 kb on + strand at 152.439 kb on - strand at 152.466 kb on - strand at 152.491 kb on + strand at 152.534 kb on + strand at 152.535 kb on - strand at 152.535 kb on - strand at 152.540 kb on + strand at 152.540 kb on + strand at 152.540 kb on + strand at 152.541 kb on - strand at 152.541 kb on - strand at 152.543 kb on + strand at 152.843 kb on - strand, within HEPCGN_07095 at 152.852 kb on + strand, within HEPCGN_07095 at 152.852 kb on + strand, within HEPCGN_07095 at 152.932 kb on - strand, within HEPCGN_07095 at 152.932 kb on - strand, within HEPCGN_07095 at 153.774 kb on + strand, within HEPCGN_07100 at 154.370 kb on + strand at 154.399 kb on - strand at 154.474 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bas51 remove 151,977 - +0.7 152,015 - nrdR HEPCGN_07085 0.18 -1.9 152,025 - nrdR HEPCGN_07085 0.20 +0.3 152,188 - nrdR HEPCGN_07085 0.56 +0.3 152,283 - nrdR HEPCGN_07085 0.77 -1.1 152,283 - nrdR HEPCGN_07085 0.77 -0.8 152,352 - -0.8 152,438 + -1.1 152,439 - +0.2 152,466 - +1.4 152,491 + -0.1 152,534 + +1.4 152,535 - +1.1 152,535 - +0.5 152,540 + +0.3 152,540 + +0.1 152,540 + +0.9 152,541 - -0.6 152,541 - +0.1 152,543 + +0.8 152,843 - HEPCGN_07095 0.17 -0.1 152,852 + HEPCGN_07095 0.18 +0.0 152,852 + HEPCGN_07095 0.18 -0.0 152,932 - HEPCGN_07095 0.28 +0.1 152,932 - HEPCGN_07095 0.28 +0.3 153,774 + HEPCGN_07100 0.57 -0.1 154,370 + +1.0 154,399 - -0.3 154,474 - +0.4
Or see this region's nucleotide sequence