Experiment: Bas51
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt bioD and clcB overlap by 48 nucleotides clcB and HEPCGN_06130 are separated by 39 nucleotides HEPCGN_06130 and dmsD are separated by 193 nucleotides dmsD and ynfH are separated by 42 nucleotides
HEPCGN_06120: bioD - dethiobiotin synthase, at 1,099,333 to 1,100,028
bioD
HEPCGN_06125: clcB - voltage-gated ClC-type chloride channel ClcB, at 1,099,981 to 1,100,772
clcB
HEPCGN_06130: HEPCGN_06130 - Putative voltage-gated ClC-type chloride channel ClcB, at 1,100,812 to 1,101,237
_06130
HEPCGN_06135: dmsD - Tat proofreading chaperone DmsD, at 1,101,431 to 1,102,045
dmsD
HEPCGN_06140: ynfH - Anaerobic dimethyl sulfoxide reductase chain YnfH, at 1,102,088 to 1,102,942
ynfH
Position (kb)
1100
1101
1102 Strain fitness (log2 ratio)
-2
-1
0
1 at 1099.972 kb on + strand at 1099.983 kb on - strand at 1099.983 kb on - strand at 1099.986 kb on + strand at 1100.014 kb on - strand at 1100.088 kb on - strand, within clcB at 1100.108 kb on - strand, within clcB at 1100.231 kb on + strand, within clcB at 1100.231 kb on + strand, within clcB at 1100.232 kb on - strand, within clcB at 1100.232 kb on - strand, within clcB at 1100.272 kb on - strand, within clcB at 1100.383 kb on + strand, within clcB at 1100.392 kb on - strand, within clcB at 1100.394 kb on - strand, within clcB at 1100.489 kb on - strand, within clcB at 1100.522 kb on - strand, within clcB at 1100.559 kb on + strand, within clcB at 1100.560 kb on - strand, within clcB at 1100.576 kb on + strand, within clcB at 1100.608 kb on + strand, within clcB at 1100.609 kb on - strand, within clcB at 1100.609 kb on - strand, within clcB at 1100.615 kb on + strand, within clcB at 1100.735 kb on - strand at 1100.735 kb on - strand at 1100.860 kb on + strand, within HEPCGN_06130 at 1100.908 kb on + strand, within HEPCGN_06130 at 1100.985 kb on - strand, within HEPCGN_06130 at 1100.985 kb on - strand, within HEPCGN_06130 at 1101.237 kb on + strand at 1101.237 kb on + strand at 1101.255 kb on - strand at 1101.262 kb on + strand at 1101.262 kb on + strand at 1101.262 kb on + strand at 1101.263 kb on - strand at 1101.263 kb on - strand at 1101.419 kb on - strand at 1101.494 kb on + strand, within dmsD at 1101.978 kb on + strand, within dmsD at 1102.049 kb on + strand at 1102.172 kb on + strand at 1102.173 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bas51 remove 1,099,972 + +0.1 1,099,983 - +0.1 1,099,983 - -0.5 1,099,986 + +1.0 1,100,014 - +0.4 1,100,088 - clcB HEPCGN_06125 0.14 -0.3 1,100,108 - clcB HEPCGN_06125 0.16 +0.3 1,100,231 + clcB HEPCGN_06125 0.32 +0.3 1,100,231 + clcB HEPCGN_06125 0.32 -0.4 1,100,232 - clcB HEPCGN_06125 0.32 +0.5 1,100,232 - clcB HEPCGN_06125 0.32 -0.5 1,100,272 - clcB HEPCGN_06125 0.37 +0.2 1,100,383 + clcB HEPCGN_06125 0.51 +0.1 1,100,392 - clcB HEPCGN_06125 0.52 -0.4 1,100,394 - clcB HEPCGN_06125 0.52 +1.2 1,100,489 - clcB HEPCGN_06125 0.64 +0.2 1,100,522 - clcB HEPCGN_06125 0.68 -0.1 1,100,559 + clcB HEPCGN_06125 0.73 +0.4 1,100,560 - clcB HEPCGN_06125 0.73 -0.2 1,100,576 + clcB HEPCGN_06125 0.75 -0.2 1,100,608 + clcB HEPCGN_06125 0.79 -0.0 1,100,609 - clcB HEPCGN_06125 0.79 +0.1 1,100,609 - clcB HEPCGN_06125 0.79 -1.3 1,100,615 + clcB HEPCGN_06125 0.80 -1.9 1,100,735 - +0.2 1,100,735 - -0.6 1,100,860 + HEPCGN_06130 0.11 +0.0 1,100,908 + HEPCGN_06130 0.23 +1.7 1,100,985 - HEPCGN_06130 0.41 -1.3 1,100,985 - HEPCGN_06130 0.41 +0.1 1,101,237 + +0.4 1,101,237 + +0.5 1,101,255 - -0.1 1,101,262 + +0.2 1,101,262 + -0.4 1,101,262 + +0.5 1,101,263 - -0.2 1,101,263 - -0.0 1,101,419 - +0.3 1,101,494 + dmsD HEPCGN_06135 0.10 +0.7 1,101,978 + dmsD HEPCGN_06135 0.89 +0.2 1,102,049 + +0.4 1,102,172 + +0.2 1,102,173 - -0.0
Or see this region's nucleotide sequence