Experiment: Bas51
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt shiA and rfaF are separated by 314 nucleotides rfaF and HEPCGN_03530 are separated by 127 nucleotides HEPCGN_03530 and HEPCGN_03535 are separated by 12 nucleotides
HEPCGN_03520: shiA - shikimate transporter, at 608,266 to 609,582
shiA
HEPCGN_03525: rfaF - Glycosyltransferase family 9 (heptosyltransferase), at 609,897 to 610,949
rfaF
HEPCGN_03530: HEPCGN_03530 - Adhesin, at 611,077 to 611,553
_03530
HEPCGN_03535: HEPCGN_03535 - adhesin, at 611,566 to 613,035
_03535
Position (kb) 609
610
611 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 608.962 kb on + strand, within shiA at 608.963 kb on - strand, within shiA at 608.963 kb on - strand, within shiA at 609.067 kb on + strand, within shiA at 609.098 kb on + strand, within shiA at 609.099 kb on - strand, within shiA at 609.253 kb on + strand, within shiA at 609.254 kb on - strand, within shiA at 609.254 kb on - strand, within shiA at 609.462 kb on - strand at 609.598 kb on - strand at 609.617 kb on + strand at 609.701 kb on + strand at 609.869 kb on + strand at 609.893 kb on - strand at 609.893 kb on - strand at 610.178 kb on - strand, within rfaF at 610.276 kb on + strand, within rfaF at 610.452 kb on + strand, within rfaF at 610.452 kb on + strand, within rfaF at 610.452 kb on + strand, within rfaF at 610.452 kb on + strand, within rfaF at 610.453 kb on - strand, within rfaF at 610.518 kb on + strand, within rfaF at 610.682 kb on + strand, within rfaF at 610.746 kb on + strand, within rfaF at 610.777 kb on + strand, within rfaF at 610.799 kb on + strand, within rfaF at 610.896 kb on - strand at 610.960 kb on - strand at 610.965 kb on + strand at 610.965 kb on + strand at 610.966 kb on - strand at 611.003 kb on + strand at 611.055 kb on + strand at 611.074 kb on + strand at 611.074 kb on + strand at 611.078 kb on + strand at 611.078 kb on + strand at 611.146 kb on + strand, within HEPCGN_03530 at 611.150 kb on - strand, within HEPCGN_03530 at 611.206 kb on + strand, within HEPCGN_03530 at 611.271 kb on + strand, within HEPCGN_03530 at 611.283 kb on - strand, within HEPCGN_03530 at 611.286 kb on - strand, within HEPCGN_03530 at 611.338 kb on - strand, within HEPCGN_03530 at 611.398 kb on - strand, within HEPCGN_03530 at 611.466 kb on + strand, within HEPCGN_03530 at 611.473 kb on - strand, within HEPCGN_03530 at 611.473 kb on - strand, within HEPCGN_03530 at 611.480 kb on + strand, within HEPCGN_03530 at 611.480 kb on + strand, within HEPCGN_03530 at 611.527 kb on - strand at 611.528 kb on + strand at 611.593 kb on + strand at 611.595 kb on + strand at 611.614 kb on + strand at 611.664 kb on + strand at 611.664 kb on + strand at 611.707 kb on + strand at 611.708 kb on - strand at 611.710 kb on - strand at 611.710 kb on - strand at 611.710 kb on - strand at 611.750 kb on - strand, within HEPCGN_03535 at 611.919 kb on + strand, within HEPCGN_03535 at 611.923 kb on + strand, within HEPCGN_03535 at 611.924 kb on - strand, within HEPCGN_03535
Per-strain Table
Position Strand Gene LocusTag Fraction Bas51 remove 608,962 + shiA HEPCGN_03520 0.53 +0.0 608,963 - shiA HEPCGN_03520 0.53 +0.1 608,963 - shiA HEPCGN_03520 0.53 -1.3 609,067 + shiA HEPCGN_03520 0.61 +0.5 609,098 + shiA HEPCGN_03520 0.63 -0.2 609,099 - shiA HEPCGN_03520 0.63 -0.9 609,253 + shiA HEPCGN_03520 0.75 +0.4 609,254 - shiA HEPCGN_03520 0.75 +0.4 609,254 - shiA HEPCGN_03520 0.75 +0.3 609,462 - -0.4 609,598 - +0.8 609,617 + -0.5 609,701 + -0.8 609,869 + +0.9 609,893 - -0.1 609,893 - -2.0 610,178 - rfaF HEPCGN_03525 0.27 -3.0 610,276 + rfaF HEPCGN_03525 0.36 +0.3 610,452 + rfaF HEPCGN_03525 0.53 -1.7 610,452 + rfaF HEPCGN_03525 0.53 +0.2 610,452 + rfaF HEPCGN_03525 0.53 -0.3 610,452 + rfaF HEPCGN_03525 0.53 -0.5 610,453 - rfaF HEPCGN_03525 0.53 -1.2 610,518 + rfaF HEPCGN_03525 0.59 -0.7 610,682 + rfaF HEPCGN_03525 0.75 +0.4 610,746 + rfaF HEPCGN_03525 0.81 -0.1 610,777 + rfaF HEPCGN_03525 0.84 -0.4 610,799 + rfaF HEPCGN_03525 0.86 +1.1 610,896 - +0.7 610,960 - -0.9 610,965 + +0.3 610,965 + -1.1 610,966 - +1.5 611,003 + -0.9 611,055 + +0.6 611,074 + -0.3 611,074 + -0.5 611,078 + -0.5 611,078 + -0.0 611,146 + HEPCGN_03530 0.14 -0.6 611,150 - HEPCGN_03530 0.15 +0.5 611,206 + HEPCGN_03530 0.27 -1.3 611,271 + HEPCGN_03530 0.41 -0.0 611,283 - HEPCGN_03530 0.43 -0.3 611,286 - HEPCGN_03530 0.44 -0.8 611,338 - HEPCGN_03530 0.55 +0.3 611,398 - HEPCGN_03530 0.67 +0.3 611,466 + HEPCGN_03530 0.82 +0.0 611,473 - HEPCGN_03530 0.83 +3.4 611,473 - HEPCGN_03530 0.83 -0.9 611,480 + HEPCGN_03530 0.84 +3.3 611,480 + HEPCGN_03530 0.84 -0.1 611,527 - +0.6 611,528 + -0.4 611,593 + -0.2 611,595 + +0.6 611,614 + -0.2 611,664 + -0.0 611,664 + -0.0 611,707 + -0.3 611,708 - +0.4 611,710 - -0.1 611,710 - +0.4 611,710 - -0.1 611,750 - HEPCGN_03535 0.13 +0.0 611,919 + HEPCGN_03535 0.24 -0.3 611,923 + HEPCGN_03535 0.24 -1.5 611,924 - HEPCGN_03535 0.24 -0.1
Or see this region's nucleotide sequence