Experiment: Guanine 0.6617 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt A4249_RS03250 and A4249_RS16495 are separated by 45 nucleotides A4249_RS16495 and A4249_RS16725 are separated by 158 nucleotides A4249_RS16725 and A4249_RS03260 overlap by 38 nucleotides A4249_RS03260 and A4249_RS03265 are separated by 283 nucleotides A4249_RS03265 and A4249_RS03270 are separated by 100 nucleotides
A4249_RS03250: A4249_RS03250 - TonB-dependent receptor, at 683,046 to 684,938
_RS03250
A4249_RS16495: A4249_RS16495 - hypothetical protein, at 684,984 to 685,178
_RS16495
A4249_RS16725: A4249_RS16725 - hypothetical protein, at 685,337 to 685,726
_RS16725
A4249_RS03260: A4249_RS03260 - acyltransferase, at 685,689 to 686,849
_RS03260
A4249_RS03265: A4249_RS03265 - metal-sensitive transcriptional regulator, at 687,133 to 687,408
_RS03265
A4249_RS03270: A4249_RS03270 - heavy metal translocating P-type ATPase, at 687,509 to 689,863
_RS03270
Position (kb) 685
686
687 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 684.715 kb on + strand, within A4249_RS03250 at 684.715 kb on + strand, within A4249_RS03250 at 684.715 kb on + strand, within A4249_RS03250 at 684.715 kb on + strand, within A4249_RS03250 at 684.715 kb on + strand, within A4249_RS03250 at 684.715 kb on + strand, within A4249_RS03250 at 684.716 kb on - strand, within A4249_RS03250 at 684.716 kb on - strand, within A4249_RS03250 at 684.716 kb on - strand, within A4249_RS03250 at 684.716 kb on - strand, within A4249_RS03250 at 684.716 kb on - strand, within A4249_RS03250 at 684.716 kb on - strand, within A4249_RS03250 at 684.716 kb on - strand, within A4249_RS03250 at 684.716 kb on - strand, within A4249_RS03250 at 684.716 kb on - strand, within A4249_RS03250 at 684.716 kb on - strand, within A4249_RS03250 at 684.716 kb on - strand, within A4249_RS03250 at 684.808 kb on + strand at 684.808 kb on + strand at 684.808 kb on + strand at 684.808 kb on + strand at 684.809 kb on - strand at 684.865 kb on + strand at 684.866 kb on - strand at 684.953 kb on + strand at 684.985 kb on + strand at 684.985 kb on + strand at 684.986 kb on - strand at 685.346 kb on + strand at 685.346 kb on + strand at 685.346 kb on + strand at 685.346 kb on + strand at 685.346 kb on + strand at 685.346 kb on + strand at 685.346 kb on + strand at 685.346 kb on + strand at 685.346 kb on + strand at 685.347 kb on - strand at 685.347 kb on - strand at 685.347 kb on - strand at 685.364 kb on + strand at 685.364 kb on + strand at 685.364 kb on + strand at 685.365 kb on - strand at 685.365 kb on - strand at 685.365 kb on - strand at 685.365 kb on - strand at 685.365 kb on - strand at 685.365 kb on - strand at 685.399 kb on - strand, within A4249_RS16725 at 685.399 kb on - strand, within A4249_RS16725 at 685.399 kb on - strand, within A4249_RS16725 at 685.773 kb on + strand at 685.945 kb on + strand, within A4249_RS03260 at 685.945 kb on + strand, within A4249_RS03260 at 685.945 kb on + strand, within A4249_RS03260 at 685.946 kb on - strand, within A4249_RS03260 at 685.946 kb on - strand, within A4249_RS03260 at 685.946 kb on - strand, within A4249_RS03260 at 685.946 kb on - strand, within A4249_RS03260 at 685.946 kb on - strand, within A4249_RS03260 at 685.946 kb on - strand, within A4249_RS03260 at 685.946 kb on - strand, within A4249_RS03260 at 685.946 kb on - strand, within A4249_RS03260 at 686.173 kb on + strand, within A4249_RS03260 at 686.173 kb on + strand, within A4249_RS03260 at 686.173 kb on + strand, within A4249_RS03260 at 686.174 kb on - strand, within A4249_RS03260 at 686.174 kb on - strand, within A4249_RS03260 at 686.174 kb on - strand, within A4249_RS03260 at 686.266 kb on + strand, within A4249_RS03260 at 686.266 kb on + strand, within A4249_RS03260 at 686.266 kb on + strand, within A4249_RS03260 at 686.266 kb on + strand, within A4249_RS03260 at 686.266 kb on + strand, within A4249_RS03260 at 686.267 kb on - strand, within A4249_RS03260 at 686.267 kb on - strand, within A4249_RS03260 at 686.267 kb on - strand, within A4249_RS03260 at 686.267 kb on - strand, within A4249_RS03260 at 686.371 kb on + strand, within A4249_RS03260 at 686.371 kb on + strand, within A4249_RS03260 at 686.372 kb on - strand, within A4249_RS03260 at 686.372 kb on - strand, within A4249_RS03260 at 686.372 kb on - strand, within A4249_RS03260 at 686.641 kb on + strand, within A4249_RS03260 at 686.641 kb on + strand, within A4249_RS03260 at 686.641 kb on + strand, within A4249_RS03260 at 686.642 kb on - strand, within A4249_RS03260 at 686.642 kb on - strand, within A4249_RS03260 at 686.642 kb on - strand, within A4249_RS03260 at 686.642 kb on - strand, within A4249_RS03260 at 686.750 kb on - strand at 686.892 kb on + strand at 686.892 kb on + strand at 686.892 kb on + strand at 686.900 kb on + strand at 686.900 kb on + strand at 686.900 kb on + strand at 686.900 kb on + strand at 686.900 kb on + strand at 686.901 kb on - strand at 686.901 kb on - strand at 686.956 kb on + strand at 686.956 kb on + strand at 686.957 kb on - strand at 686.957 kb on - strand at 686.957 kb on - strand at 686.957 kb on - strand at 687.315 kb on - strand, within A4249_RS03265 at 687.361 kb on + strand, within A4249_RS03265 at 687.362 kb on - strand, within A4249_RS03265 at 687.437 kb on + strand at 687.437 kb on + strand at 687.437 kb on + strand at 687.437 kb on + strand at 687.437 kb on + strand at 687.438 kb on - strand at 687.438 kb on - strand at 687.438 kb on - strand at 687.438 kb on - strand at 687.438 kb on - strand at 687.477 kb on + strand at 687.478 kb on - strand at 687.478 kb on - strand at 687.485 kb on - strand at 687.514 kb on + strand at 687.514 kb on + strand at 687.514 kb on + strand at 687.514 kb on + strand at 687.514 kb on + strand at 687.514 kb on + strand at 687.515 kb on - strand at 687.515 kb on - strand at 687.515 kb on - strand at 687.515 kb on - strand at 687.515 kb on - strand at 687.515 kb on - strand at 687.659 kb on + strand at 687.659 kb on + strand at 687.659 kb on + strand at 687.659 kb on + strand at 687.659 kb on + strand at 687.659 kb on + strand at 687.659 kb on + strand at 687.659 kb on + strand at 687.659 kb on + strand at 687.659 kb on + strand at 687.659 kb on + strand at 687.659 kb on + strand at 687.659 kb on + strand at 687.659 kb on + strand at 687.659 kb on + strand at 687.659 kb on + strand at 687.659 kb on + strand at 687.659 kb on + strand at 687.659 kb on + strand at 687.659 kb on + strand at 687.659 kb on + strand at 687.659 kb on + strand at 687.659 kb on + strand at 687.659 kb on + strand at 687.660 kb on - strand at 687.660 kb on - strand at 687.660 kb on - strand at 687.660 kb on - strand at 687.660 kb on - strand at 687.660 kb on - strand at 687.660 kb on - strand at 687.660 kb on - strand at 687.660 kb on - strand at 687.660 kb on - strand at 687.660 kb on - strand at 687.660 kb on - strand at 687.714 kb on - strand at 687.714 kb on - strand at 687.714 kb on - strand at 687.714 kb on - strand at 687.714 kb on - strand at 687.761 kb on + strand, within A4249_RS03270 at 687.761 kb on + strand, within A4249_RS03270 at 687.761 kb on + strand, within A4249_RS03270 at 687.761 kb on + strand, within A4249_RS03270 at 687.761 kb on + strand, within A4249_RS03270 at 687.761 kb on + strand, within A4249_RS03270 at 687.761 kb on + strand, within A4249_RS03270 at 687.761 kb on + strand, within A4249_RS03270 at 687.761 kb on + strand, within A4249_RS03270 at 687.762 kb on - strand, within A4249_RS03270 at 687.762 kb on - strand, within A4249_RS03270 at 687.762 kb on - strand, within A4249_RS03270 at 687.762 kb on - strand, within A4249_RS03270 at 687.762 kb on - strand, within A4249_RS03270 at 687.762 kb on - strand, within A4249_RS03270 at 687.762 kb on - strand, within A4249_RS03270 at 687.762 kb on - strand, within A4249_RS03270 at 687.762 kb on - strand, within A4249_RS03270 at 687.762 kb on - strand, within A4249_RS03270 at 687.762 kb on - strand, within A4249_RS03270 at 687.762 kb on - strand, within A4249_RS03270
Per-strain Table
Position Strand Gene LocusTag Fraction Guanine 0.6617 mM (C) remove 684,715 + A4249_RS03250 0.88 +1.0 684,715 + A4249_RS03250 0.88 -0.5 684,715 + A4249_RS03250 0.88 -0.5 684,715 + A4249_RS03250 0.88 +0.2 684,715 + A4249_RS03250 0.88 -0.4 684,715 + A4249_RS03250 0.88 +0.3 684,716 - A4249_RS03250 0.88 -0.7 684,716 - A4249_RS03250 0.88 -0.3 684,716 - A4249_RS03250 0.88 +0.2 684,716 - A4249_RS03250 0.88 -0.5 684,716 - A4249_RS03250 0.88 -1.0 684,716 - A4249_RS03250 0.88 +1.3 684,716 - A4249_RS03250 0.88 -2.0 684,716 - A4249_RS03250 0.88 -0.1 684,716 - A4249_RS03250 0.88 +0.1 684,716 - A4249_RS03250 0.88 +0.6 684,716 - A4249_RS03250 0.88 +0.7 684,808 + -0.3 684,808 + +0.3 684,808 + +0.1 684,808 + +0.1 684,809 - +0.2 684,865 + +0.8 684,866 - +0.3 684,953 + +0.7 684,985 + +0.0 684,985 + +0.7 684,986 - -0.3 685,346 + -0.2 685,346 + -2.7 685,346 + +0.0 685,346 + +0.0 685,346 + -0.8 685,346 + -0.2 685,346 + +0.0 685,346 + -0.6 685,346 + -0.3 685,347 - +1.6 685,347 - +0.0 685,347 - +0.2 685,364 + +0.5 685,364 + -1.4 685,364 + -0.7 685,365 - -1.2 685,365 - -0.5 685,365 - +0.4 685,365 - +1.0 685,365 - -1.2 685,365 - +0.0 685,399 - A4249_RS16725 0.16 -1.8 685,399 - A4249_RS16725 0.16 -0.2 685,399 - A4249_RS16725 0.16 -1.1 685,773 + +0.2 685,945 + A4249_RS03260 0.22 +1.0 685,945 + A4249_RS03260 0.22 +0.1 685,945 + A4249_RS03260 0.22 +0.1 685,946 - A4249_RS03260 0.22 -0.2 685,946 - A4249_RS03260 0.22 -0.4 685,946 - A4249_RS03260 0.22 +0.4 685,946 - A4249_RS03260 0.22 +0.4 685,946 - A4249_RS03260 0.22 -0.1 685,946 - A4249_RS03260 0.22 -0.4 685,946 - A4249_RS03260 0.22 +0.3 685,946 - A4249_RS03260 0.22 +0.6 686,173 + A4249_RS03260 0.42 +1.0 686,173 + A4249_RS03260 0.42 +1.4 686,173 + A4249_RS03260 0.42 -0.2 686,174 - A4249_RS03260 0.42 +0.1 686,174 - A4249_RS03260 0.42 +0.2 686,174 - A4249_RS03260 0.42 -0.3 686,266 + A4249_RS03260 0.50 -0.1 686,266 + A4249_RS03260 0.50 -0.6 686,266 + A4249_RS03260 0.50 +0.0 686,266 + A4249_RS03260 0.50 +0.2 686,266 + A4249_RS03260 0.50 -0.3 686,267 - A4249_RS03260 0.50 +0.3 686,267 - A4249_RS03260 0.50 -0.7 686,267 - A4249_RS03260 0.50 +0.4 686,267 - A4249_RS03260 0.50 -0.4 686,371 + A4249_RS03260 0.59 +0.9 686,371 + A4249_RS03260 0.59 -0.1 686,372 - A4249_RS03260 0.59 +0.1 686,372 - A4249_RS03260 0.59 +0.2 686,372 - A4249_RS03260 0.59 -0.5 686,641 + A4249_RS03260 0.82 +1.0 686,641 + A4249_RS03260 0.82 -0.3 686,641 + A4249_RS03260 0.82 -2.5 686,642 - A4249_RS03260 0.82 -1.0 686,642 - A4249_RS03260 0.82 -1.0 686,642 - A4249_RS03260 0.82 -0.8 686,642 - A4249_RS03260 0.82 -0.7 686,750 - +0.4 686,892 + +0.3 686,892 + -0.4 686,892 + -0.9 686,900 + -0.3 686,900 + +0.3 686,900 + -0.2 686,900 + -0.7 686,900 + -0.2 686,901 - +0.0 686,901 - +0.4 686,956 + -0.9 686,956 + +0.8 686,957 - -0.3 686,957 - +0.7 686,957 - +0.3 686,957 - -0.2 687,315 - A4249_RS03265 0.66 -0.6 687,361 + A4249_RS03265 0.83 +0.5 687,362 - A4249_RS03265 0.83 -0.1 687,437 + -0.2 687,437 + +0.5 687,437 + +0.3 687,437 + +0.5 687,437 + +2.6 687,438 - +0.1 687,438 - -0.1 687,438 - -2.4 687,438 - -0.5 687,438 - -0.1 687,477 + +0.2 687,478 - +0.1 687,478 - +0.2 687,485 - -0.4 687,514 + +0.9 687,514 + -0.5 687,514 + -0.4 687,514 + +0.9 687,514 + +0.5 687,514 + +3.1 687,515 - +1.0 687,515 - -0.4 687,515 - -0.3 687,515 - -0.5 687,515 - +0.3 687,515 - -0.7 687,659 + -0.1 687,659 + +0.0 687,659 + -0.4 687,659 + -0.6 687,659 + +1.0 687,659 + +1.2 687,659 + -0.9 687,659 + +0.1 687,659 + +0.0 687,659 + +0.1 687,659 + +0.4 687,659 + -0.3 687,659 + +0.2 687,659 + +0.7 687,659 + -0.3 687,659 + +0.7 687,659 + -0.2 687,659 + +0.3 687,659 + +0.6 687,659 + -0.1 687,659 + +1.4 687,659 + -0.1 687,659 + -0.1 687,659 + +0.3 687,660 - -0.0 687,660 - +0.2 687,660 - -0.8 687,660 - -0.3 687,660 - -1.6 687,660 - +0.4 687,660 - -0.6 687,660 - +0.6 687,660 - +0.4 687,660 - +0.8 687,660 - +0.1 687,660 - +0.7 687,714 - -0.2 687,714 - -0.3 687,714 - +0.2 687,714 - +0.4 687,714 - -0.3 687,761 + A4249_RS03270 0.11 +1.0 687,761 + A4249_RS03270 0.11 +0.4 687,761 + A4249_RS03270 0.11 +1.6 687,761 + A4249_RS03270 0.11 -0.4 687,761 + A4249_RS03270 0.11 -0.8 687,761 + A4249_RS03270 0.11 -0.1 687,761 + A4249_RS03270 0.11 -0.1 687,761 + A4249_RS03270 0.11 +0.4 687,761 + A4249_RS03270 0.11 +0.2 687,762 - A4249_RS03270 0.11 -2.1 687,762 - A4249_RS03270 0.11 -0.0 687,762 - A4249_RS03270 0.11 +1.6 687,762 - A4249_RS03270 0.11 +0.7 687,762 - A4249_RS03270 0.11 -0.3 687,762 - A4249_RS03270 0.11 -0.3 687,762 - A4249_RS03270 0.11 -1.9 687,762 - A4249_RS03270 0.11 -1.9 687,762 - A4249_RS03270 0.11 +1.2 687,762 - A4249_RS03270 0.11 -0.0 687,762 - A4249_RS03270 0.11 +0.4 687,762 - A4249_RS03270 0.11 +0.7
Or see this region's nucleotide sequence