Experiment: L-Arabinose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rfaG and rfaP overlap by 8 nucleotides rfaP and waaO are separated by 42 nucleotides waaO and rfaY are separated by 25 nucleotides rfaY and rfaJ are separated by 24 nucleotides
NIAGMN_13415: rfaG - Lipopolysaccharide core biosynthesis protein RfaG, at 2,650,801 to 2,651,925
rfaG
NIAGMN_13420: rfaP - lipopolysaccharide core heptose(I) kinase RfaP, at 2,651,918 to 2,652,715
rfaP
NIAGMN_13425: waaO - lipopolysaccharide 3-alpha-galactosyltransferase, at 2,652,758 to 2,653,765
waaO
NIAGMN_13430: rfaY - lipopolysaccharide core heptose(II) kinase RfaY, at 2,653,791 to 2,654,498
rfaY
NIAGMN_13435: rfaJ - glucosyltransferase, at 2,654,523 to 2,655,536
rfaJ
Position (kb)
2652
2653
2654 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2651.852 kb on - strand at 2651.860 kb on - strand at 2651.860 kb on - strand at 2651.871 kb on - strand at 2651.871 kb on - strand at 2651.873 kb on - strand at 2651.903 kb on + strand at 2651.904 kb on - strand at 2651.980 kb on + strand at 2652.056 kb on + strand, within rfaP at 2652.057 kb on - strand, within rfaP at 2652.190 kb on + strand, within rfaP at 2652.190 kb on + strand, within rfaP at 2652.191 kb on - strand, within rfaP at 2652.194 kb on + strand, within rfaP at 2652.194 kb on + strand, within rfaP at 2652.194 kb on + strand, within rfaP at 2652.194 kb on + strand, within rfaP at 2652.195 kb on - strand, within rfaP at 2652.195 kb on - strand, within rfaP at 2652.247 kb on + strand, within rfaP at 2652.248 kb on - strand, within rfaP at 2652.248 kb on - strand, within rfaP at 2652.276 kb on + strand, within rfaP at 2652.277 kb on - strand, within rfaP at 2652.277 kb on - strand, within rfaP at 2652.338 kb on - strand, within rfaP at 2652.344 kb on - strand, within rfaP at 2652.346 kb on + strand, within rfaP at 2652.346 kb on + strand, within rfaP at 2652.373 kb on + strand, within rfaP at 2652.373 kb on + strand, within rfaP at 2652.407 kb on + strand, within rfaP at 2652.408 kb on - strand, within rfaP at 2652.408 kb on - strand, within rfaP at 2652.410 kb on - strand, within rfaP at 2652.410 kb on - strand, within rfaP at 2652.535 kb on + strand, within rfaP at 2652.754 kb on - strand at 2652.885 kb on + strand, within waaO at 2653.117 kb on + strand, within waaO at 2653.201 kb on + strand, within waaO at 2653.428 kb on + strand, within waaO at 2653.483 kb on + strand, within waaO at 2653.542 kb on + strand, within waaO at 2653.542 kb on + strand, within waaO at 2653.547 kb on + strand, within waaO at 2653.547 kb on + strand, within waaO at 2653.547 kb on + strand, within waaO at 2653.663 kb on + strand, within waaO at 2653.913 kb on + strand, within rfaY at 2654.144 kb on + strand, within rfaY at 2654.144 kb on + strand, within rfaY at 2654.390 kb on + strand, within rfaY at 2654.506 kb on - strand at 2654.719 kb on - strand, within rfaJ
Per-strain Table
Position Strand Gene LocusTag Fraction L-Arabinose remove 2,651,852 - +1.5 2,651,860 - -0.2 2,651,860 - -0.3 2,651,871 - -0.6 2,651,871 - -0.7 2,651,873 - -1.3 2,651,903 + -1.5 2,651,904 - -0.5 2,651,980 + +0.3 2,652,056 + rfaP NIAGMN_13420 0.17 -0.5 2,652,057 - rfaP NIAGMN_13420 0.17 -0.9 2,652,190 + rfaP NIAGMN_13420 0.34 +2.1 2,652,190 + rfaP NIAGMN_13420 0.34 +1.3 2,652,191 - rfaP NIAGMN_13420 0.34 +2.0 2,652,194 + rfaP NIAGMN_13420 0.35 -0.9 2,652,194 + rfaP NIAGMN_13420 0.35 -1.3 2,652,194 + rfaP NIAGMN_13420 0.35 +0.6 2,652,194 + rfaP NIAGMN_13420 0.35 -0.8 2,652,195 - rfaP NIAGMN_13420 0.35 -0.5 2,652,195 - rfaP NIAGMN_13420 0.35 -1.0 2,652,247 + rfaP NIAGMN_13420 0.41 +0.7 2,652,248 - rfaP NIAGMN_13420 0.41 -2.7 2,652,248 - rfaP NIAGMN_13420 0.41 -0.5 2,652,276 + rfaP NIAGMN_13420 0.45 -0.2 2,652,277 - rfaP NIAGMN_13420 0.45 -0.3 2,652,277 - rfaP NIAGMN_13420 0.45 +2.0 2,652,338 - rfaP NIAGMN_13420 0.53 +1.0 2,652,344 - rfaP NIAGMN_13420 0.53 -0.8 2,652,346 + rfaP NIAGMN_13420 0.54 -1.2 2,652,346 + rfaP NIAGMN_13420 0.54 -0.7 2,652,373 + rfaP NIAGMN_13420 0.57 -0.8 2,652,373 + rfaP NIAGMN_13420 0.57 +0.3 2,652,407 + rfaP NIAGMN_13420 0.61 +0.6 2,652,408 - rfaP NIAGMN_13420 0.61 +1.3 2,652,408 - rfaP NIAGMN_13420 0.61 -1.0 2,652,410 - rfaP NIAGMN_13420 0.62 +0.3 2,652,410 - rfaP NIAGMN_13420 0.62 +1.7 2,652,535 + rfaP NIAGMN_13420 0.77 -2.1 2,652,754 - +2.9 2,652,885 + waaO NIAGMN_13425 0.13 +0.6 2,653,117 + waaO NIAGMN_13425 0.36 -0.8 2,653,201 + waaO NIAGMN_13425 0.44 -0.3 2,653,428 + waaO NIAGMN_13425 0.66 -0.7 2,653,483 + waaO NIAGMN_13425 0.72 -2.6 2,653,542 + waaO NIAGMN_13425 0.78 +0.2 2,653,542 + waaO NIAGMN_13425 0.78 +0.1 2,653,547 + waaO NIAGMN_13425 0.78 +2.5 2,653,547 + waaO NIAGMN_13425 0.78 -0.7 2,653,547 + waaO NIAGMN_13425 0.78 -0.1 2,653,663 + waaO NIAGMN_13425 0.90 -0.0 2,653,913 + rfaY NIAGMN_13430 0.17 -0.1 2,654,144 + rfaY NIAGMN_13430 0.50 +2.1 2,654,144 + rfaY NIAGMN_13430 0.50 +0.8 2,654,390 + rfaY NIAGMN_13430 0.85 -1.3 2,654,506 - -1.0 2,654,719 - rfaJ NIAGMN_13435 0.19 -1.2
Or see this region's nucleotide sequence