Experiment: L-Arabinose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ansA and pncA are separated by 10 nucleotides pncA and ydjE are separated by 92 nucleotides ydjE and tnp-IS629 are separated by 152 nucleotides tnp-IS629 and MCAODC_18625 overlap by 1 nucleotides
MCAODC_18605: ansA - asparaginase, at 580,431 to 581,447
ansA
MCAODC_18610: pncA - bifunctional nicotinamidase/pyrazinamidase, at 581,458 to 582,099
pncA
MCAODC_18615: ydjE - Inner membrane metabolite transport protein YdjE, at 582,192 to 583,352
ydjE
MCAODC_18620: tnp-IS629 - IS3 family IS629 transposase, at 583,505 to 583,831
tnp-IS629
MCAODC_18625: MCAODC_18625 - IS3 family transposase, at 583,831 to 584,718
_18625
Position (kb) 582
583
584 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 581.249 kb on + strand, within ansA at 581.249 kb on + strand, within ansA at 581.249 kb on + strand, within ansA at 581.249 kb on + strand, within ansA at 581.335 kb on + strand, within ansA at 581.335 kb on + strand, within ansA at 581.361 kb on + strand at 581.541 kb on + strand, within pncA at 581.541 kb on + strand, within pncA at 581.541 kb on + strand, within pncA at 581.668 kb on - strand, within pncA at 581.745 kb on - strand, within pncA at 581.816 kb on - strand, within pncA at 581.957 kb on + strand, within pncA at 581.957 kb on + strand, within pncA at 581.964 kb on + strand, within pncA at 582.062 kb on + strand at 582.066 kb on + strand at 582.066 kb on + strand at 582.067 kb on - strand at 582.067 kb on - strand at 582.104 kb on - strand at 582.124 kb on - strand at 582.322 kb on - strand, within ydjE at 582.454 kb on + strand, within ydjE at 582.455 kb on - strand, within ydjE at 582.461 kb on - strand, within ydjE at 582.461 kb on - strand, within ydjE at 582.524 kb on - strand, within ydjE at 582.527 kb on + strand, within ydjE at 582.528 kb on - strand, within ydjE at 582.764 kb on + strand, within ydjE at 582.891 kb on + strand, within ydjE at 582.892 kb on - strand, within ydjE at 583.086 kb on - strand, within ydjE at 583.211 kb on + strand, within ydjE at 583.220 kb on - strand, within ydjE at 583.223 kb on + strand, within ydjE at 583.223 kb on + strand, within ydjE at 583.224 kb on - strand, within ydjE at 583.276 kb on - strand at 583.276 kb on - strand at 583.416 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Arabinose remove 581,249 + ansA MCAODC_18605 0.80 +0.3 581,249 + ansA MCAODC_18605 0.80 +0.4 581,249 + ansA MCAODC_18605 0.80 +0.4 581,249 + ansA MCAODC_18605 0.80 +0.4 581,335 + ansA MCAODC_18605 0.89 +0.3 581,335 + ansA MCAODC_18605 0.89 -0.9 581,361 + +0.1 581,541 + pncA MCAODC_18610 0.13 -1.1 581,541 + pncA MCAODC_18610 0.13 +0.4 581,541 + pncA MCAODC_18610 0.13 +0.2 581,668 - pncA MCAODC_18610 0.33 +0.7 581,745 - pncA MCAODC_18610 0.45 +3.1 581,816 - pncA MCAODC_18610 0.56 -2.1 581,957 + pncA MCAODC_18610 0.78 -0.9 581,957 + pncA MCAODC_18610 0.78 -0.2 581,964 + pncA MCAODC_18610 0.79 -2.4 582,062 + -0.2 582,066 + -0.2 582,066 + -0.1 582,067 - -0.9 582,067 - +1.5 582,104 - +0.5 582,124 - -0.0 582,322 - ydjE MCAODC_18615 0.11 -0.4 582,454 + ydjE MCAODC_18615 0.23 -0.9 582,455 - ydjE MCAODC_18615 0.23 +1.1 582,461 - ydjE MCAODC_18615 0.23 +1.1 582,461 - ydjE MCAODC_18615 0.23 -1.3 582,524 - ydjE MCAODC_18615 0.29 +1.0 582,527 + ydjE MCAODC_18615 0.29 -2.3 582,528 - ydjE MCAODC_18615 0.29 -0.9 582,764 + ydjE MCAODC_18615 0.49 -0.6 582,891 + ydjE MCAODC_18615 0.60 -1.1 582,892 - ydjE MCAODC_18615 0.60 +0.2 583,086 - ydjE MCAODC_18615 0.77 -1.6 583,211 + ydjE MCAODC_18615 0.88 -0.9 583,220 - ydjE MCAODC_18615 0.89 -0.9 583,223 + ydjE MCAODC_18615 0.89 +0.2 583,223 + ydjE MCAODC_18615 0.89 -0.5 583,224 - ydjE MCAODC_18615 0.89 +1.2 583,276 - +1.6 583,276 - -1.7 583,416 + -2.0
Or see this region's nucleotide sequence