Experiment: L-Arabinose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ynbD and azoR are separated by 50 nucleotides azoR and hrpA are separated by 200 nucleotides hrpA and MCAODC_16210 are separated by 145 nucleotides MCAODC_16210 and MCAODC_16215 are separated by 4 nucleotides MCAODC_16215 and MCAODC_16220 overlap by 14 nucleotides
MCAODC_16195: ynbD - Uncharacterized protein YnbD, at 134,281 to 135,597
ynbD
MCAODC_16200: azoR - FMN-dependent NADH-azoreductase, at 135,648 to 136,253
azoR
MCAODC_16205: hrpA - ATP-dependent RNA helicase HrpA, at 136,454 to 140,356
hrpA
MCAODC_16210: MCAODC_16210 - hypothetical protein, at 140,502 to 140,927
_16210
MCAODC_16215: MCAODC_16215 - hypothetical protein, at 140,932 to 141,270
_16215
MCAODC_16220: MCAODC_16220 - hypothetical protein, at 141,257 to 141,688
_16220
Position (kb) 136
137
138
139
140
141 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 135.479 kb on + strand at 135.485 kb on - strand at 135.495 kb on + strand at 135.543 kb on + strand at 135.552 kb on + strand at 135.663 kb on - strand at 135.755 kb on + strand, within azoR at 135.787 kb on + strand, within azoR at 135.788 kb on - strand, within azoR at 135.842 kb on + strand, within azoR at 135.890 kb on + strand, within azoR at 135.903 kb on - strand, within azoR at 135.964 kb on + strand, within azoR at 135.964 kb on + strand, within azoR at 135.965 kb on - strand, within azoR at 136.242 kb on + strand at 136.271 kb on - strand at 136.308 kb on + strand at 136.331 kb on + strand at 136.395 kb on + strand at 136.400 kb on + strand at 136.400 kb on + strand at 136.401 kb on - strand at 136.407 kb on - strand at 137.176 kb on + strand, within hrpA at 137.307 kb on + strand, within hrpA at 137.533 kb on + strand, within hrpA at 137.537 kb on + strand, within hrpA at 137.537 kb on + strand, within hrpA at 137.573 kb on + strand, within hrpA at 138.013 kb on + strand, within hrpA at 138.014 kb on - strand, within hrpA at 138.215 kb on + strand, within hrpA at 138.216 kb on - strand, within hrpA at 138.245 kb on + strand, within hrpA at 138.245 kb on + strand, within hrpA at 138.383 kb on + strand, within hrpA at 138.386 kb on - strand, within hrpA at 138.559 kb on + strand, within hrpA at 139.081 kb on - strand, within hrpA at 139.081 kb on - strand, within hrpA at 139.084 kb on + strand, within hrpA at 139.174 kb on + strand, within hrpA at 139.294 kb on - strand, within hrpA at 139.464 kb on + strand, within hrpA at 139.464 kb on + strand, within hrpA at 139.507 kb on + strand, within hrpA at 139.508 kb on - strand, within hrpA at 139.538 kb on + strand, within hrpA at 139.538 kb on + strand, within hrpA at 139.559 kb on + strand, within hrpA at 139.789 kb on + strand, within hrpA at 139.979 kb on - strand at 140.031 kb on + strand at 140.032 kb on - strand at 140.323 kb on + strand at 140.324 kb on - strand at 140.399 kb on - strand at 140.458 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Arabinose remove 135,479 + +0.5 135,485 - -3.1 135,495 + -1.2 135,543 + -2.8 135,552 + -0.9 135,663 - -2.0 135,755 + azoR MCAODC_16200 0.18 -1.3 135,787 + azoR MCAODC_16200 0.23 -1.2 135,788 - azoR MCAODC_16200 0.23 +0.7 135,842 + azoR MCAODC_16200 0.32 +1.3 135,890 + azoR MCAODC_16200 0.40 -0.3 135,903 - azoR MCAODC_16200 0.42 -0.9 135,964 + azoR MCAODC_16200 0.52 -1.9 135,964 + azoR MCAODC_16200 0.52 +0.4 135,965 - azoR MCAODC_16200 0.52 +0.4 136,242 + -0.3 136,271 - +2.0 136,308 + -0.8 136,331 + +0.3 136,395 + -0.7 136,400 + +2.1 136,400 + +1.1 136,401 - +1.0 136,407 - +2.6 137,176 + hrpA MCAODC_16205 0.18 +1.7 137,307 + hrpA MCAODC_16205 0.22 -3.1 137,533 + hrpA MCAODC_16205 0.28 +0.7 137,537 + hrpA MCAODC_16205 0.28 -1.7 137,537 + hrpA MCAODC_16205 0.28 -1.9 137,573 + hrpA MCAODC_16205 0.29 -0.1 138,013 + hrpA MCAODC_16205 0.40 +1.2 138,014 - hrpA MCAODC_16205 0.40 +2.0 138,215 + hrpA MCAODC_16205 0.45 +0.0 138,216 - hrpA MCAODC_16205 0.45 +0.4 138,245 + hrpA MCAODC_16205 0.46 -0.6 138,245 + hrpA MCAODC_16205 0.46 +1.0 138,383 + hrpA MCAODC_16205 0.49 -0.9 138,386 - hrpA MCAODC_16205 0.50 -1.6 138,559 + hrpA MCAODC_16205 0.54 -1.6 139,081 - hrpA MCAODC_16205 0.67 +1.0 139,081 - hrpA MCAODC_16205 0.67 -0.7 139,084 + hrpA MCAODC_16205 0.67 -0.7 139,174 + hrpA MCAODC_16205 0.70 -1.0 139,294 - hrpA MCAODC_16205 0.73 +0.5 139,464 + hrpA MCAODC_16205 0.77 +0.2 139,464 + hrpA MCAODC_16205 0.77 +0.4 139,507 + hrpA MCAODC_16205 0.78 -0.3 139,508 - hrpA MCAODC_16205 0.78 -2.7 139,538 + hrpA MCAODC_16205 0.79 -1.0 139,538 + hrpA MCAODC_16205 0.79 -2.8 139,559 + hrpA MCAODC_16205 0.80 +1.7 139,789 + hrpA MCAODC_16205 0.85 +1.4 139,979 - -0.8 140,031 + -2.5 140,032 - -0.6 140,323 + -0.5 140,324 - -1.4 140,399 - +0.5 140,458 - +1.2
Or see this region's nucleotide sequence