Strain Fitness in Escherichia coli ECRC101 around MCAODC_06195

Experiment: L-Arabinose

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntrfaB and rfaL are separated by 35 nucleotidesrfaL and rfaC overlap by 4 nucleotidesrfaC and rfaF are separated by 3 nucleotides MCAODC_06190: rfaB - UDP-glucose--(glucosyl)LPS alpha-1,2-glucosyltransferase, at 1,239,984 to 1,241,126 rfaB MCAODC_06195: rfaL - O-antigen ligase RfaL, at 1,241,162 to 1,242,370 rfaL MCAODC_06200: rfaC - lipopolysaccharide heptosyltransferase RfaC, at 1,242,367 to 1,243,359 rfaC MCAODC_06205: rfaF - ADP-heptose--LPS heptosyltransferase RfaF, at 1,243,363 to 1,244,409 rfaF Position (kb) 1241 1242 1243Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 1240.304 kb on + strand, within rfaBat 1240.364 kb on + strand, within rfaBat 1240.426 kb on + strand, within rfaBat 1240.544 kb on + strand, within rfaBat 1240.683 kb on - strand, within rfaBat 1240.763 kb on + strand, within rfaBat 1240.764 kb on - strand, within rfaBat 1240.764 kb on - strand, within rfaBat 1240.764 kb on - strand, within rfaBat 1240.768 kb on + strand, within rfaBat 1240.800 kb on + strand, within rfaBat 1240.875 kb on - strand, within rfaBat 1240.886 kb on + strand, within rfaBat 1240.886 kb on + strand, within rfaBat 1240.967 kb on + strand, within rfaBat 1241.068 kb on - strandat 1241.111 kb on + strandat 1241.277 kb on + strandat 1241.305 kb on + strand, within rfaLat 1241.359 kb on + strand, within rfaLat 1241.375 kb on + strand, within rfaLat 1241.423 kb on + strand, within rfaLat 1241.483 kb on - strand, within rfaLat 1241.505 kb on + strand, within rfaLat 1241.529 kb on - strand, within rfaLat 1241.579 kb on - strand, within rfaLat 1241.579 kb on - strand, within rfaLat 1241.598 kb on + strand, within rfaLat 1241.599 kb on - strand, within rfaLat 1241.928 kb on + strand, within rfaLat 1242.058 kb on + strand, within rfaLat 1242.175 kb on + strand, within rfaLat 1242.189 kb on + strand, within rfaLat 1242.228 kb on - strand, within rfaL

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Arabinose
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1,240,304 + rfaB MCAODC_06190 0.28 +0.1
1,240,364 + rfaB MCAODC_06190 0.33 -1.5
1,240,426 + rfaB MCAODC_06190 0.39 +1.4
1,240,544 + rfaB MCAODC_06190 0.49 +1.6
1,240,683 - rfaB MCAODC_06190 0.61 -1.5
1,240,763 + rfaB MCAODC_06190 0.68 +0.5
1,240,764 - rfaB MCAODC_06190 0.68 +1.9
1,240,764 - rfaB MCAODC_06190 0.68 -0.4
1,240,764 - rfaB MCAODC_06190 0.68 +1.0
1,240,768 + rfaB MCAODC_06190 0.69 +0.7
1,240,800 + rfaB MCAODC_06190 0.71 +0.5
1,240,875 - rfaB MCAODC_06190 0.78 +0.4
1,240,886 + rfaB MCAODC_06190 0.79 -0.7
1,240,886 + rfaB MCAODC_06190 0.79 +1.9
1,240,967 + rfaB MCAODC_06190 0.86 +1.5
1,241,068 - +0.5
1,241,111 + -1.0
1,241,277 + +0.8
1,241,305 + rfaL MCAODC_06195 0.12 +2.5
1,241,359 + rfaL MCAODC_06195 0.16 +0.4
1,241,375 + rfaL MCAODC_06195 0.18 +1.4
1,241,423 + rfaL MCAODC_06195 0.22 +1.0
1,241,483 - rfaL MCAODC_06195 0.27 +0.5
1,241,505 + rfaL MCAODC_06195 0.28 +3.2
1,241,529 - rfaL MCAODC_06195 0.30 -0.2
1,241,579 - rfaL MCAODC_06195 0.34 +1.3
1,241,579 - rfaL MCAODC_06195 0.34 +0.6
1,241,598 + rfaL MCAODC_06195 0.36 +0.1
1,241,599 - rfaL MCAODC_06195 0.36 -3.0
1,241,928 + rfaL MCAODC_06195 0.63 +0.7
1,242,058 + rfaL MCAODC_06195 0.74 +1.1
1,242,175 + rfaL MCAODC_06195 0.84 +0.8
1,242,189 + rfaL MCAODC_06195 0.85 +1.0
1,242,228 - rfaL MCAODC_06195 0.88 -0.0

Or see this region's nucleotide sequence