Experiment: L-Arabinose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt plsB and ubiA are separated by 154 nucleotides ubiA and ubiC are separated by 12 nucleotides ubiC and espX4 are separated by 222 nucleotides
MCAODC_03575: plsB - glycerol-3-phosphate 1-O-acyltransferase PlsB, at 715,263 to 717,686
plsB
MCAODC_03580: ubiA - 4-hydroxybenzoate octaprenyltransferase, at 717,841 to 718,713
ubiA
MCAODC_03585: ubiC - chorismate lyase, at 718,726 to 719,223
ubiC
MCAODC_03590: espX4 - Type III secretion system effector EspX4, at 719,446 to 721,026
espX4
Position (kb) 717
718
719 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 717.391 kb on - strand, within plsB at 717.635 kb on + strand at 717.635 kb on + strand at 717.635 kb on + strand at 717.636 kb on - strand at 717.735 kb on + strand at 717.735 kb on + strand at 717.735 kb on + strand at 717.770 kb on + strand at 717.834 kb on + strand at 717.854 kb on + strand at 717.855 kb on - strand at 718.728 kb on - strand at 718.731 kb on - strand at 718.731 kb on - strand at 718.731 kb on - strand at 718.763 kb on - strand at 718.850 kb on - strand, within ubiC at 718.872 kb on - strand, within ubiC at 718.872 kb on - strand, within ubiC at 718.872 kb on - strand, within ubiC at 718.880 kb on - strand, within ubiC at 718.891 kb on - strand, within ubiC at 718.925 kb on - strand, within ubiC at 718.925 kb on - strand, within ubiC at 718.948 kb on - strand, within ubiC at 718.982 kb on - strand, within ubiC at 718.997 kb on - strand, within ubiC at 719.178 kb on + strand at 719.184 kb on - strand at 719.184 kb on - strand at 719.184 kb on - strand at 719.184 kb on - strand at 719.239 kb on - strand at 719.243 kb on - strand at 719.243 kb on - strand at 719.247 kb on + strand at 719.247 kb on + strand at 719.248 kb on - strand at 719.248 kb on - strand at 719.248 kb on - strand at 719.258 kb on + strand at 719.275 kb on + strand at 719.286 kb on + strand at 719.286 kb on + strand at 719.286 kb on + strand at 719.297 kb on - strand at 719.360 kb on + strand at 719.361 kb on - strand at 719.361 kb on - strand at 719.447 kb on + strand at 719.447 kb on + strand at 719.447 kb on + strand at 719.458 kb on - strand at 719.467 kb on - strand at 719.472 kb on + strand at 719.473 kb on - strand at 719.514 kb on + strand at 719.514 kb on + strand at 719.585 kb on + strand at 719.585 kb on + strand at 719.586 kb on - strand at 719.586 kb on - strand at 719.586 kb on - strand at 719.586 kb on - strand at 719.589 kb on + strand at 719.590 kb on - strand at 719.590 kb on - strand at 719.591 kb on + strand at 719.591 kb on + strand at 719.591 kb on + strand at 719.591 kb on + strand at 719.591 kb on + strand at 719.592 kb on - strand at 719.592 kb on - strand at 719.592 kb on - strand at 719.592 kb on - strand at 719.619 kb on + strand, within espX4 at 719.631 kb on + strand, within espX4 at 719.631 kb on + strand, within espX4 at 719.631 kb on + strand, within espX4 at 719.632 kb on - strand, within espX4 at 719.638 kb on + strand, within espX4 at 719.639 kb on - strand, within espX4 at 719.662 kb on - strand, within espX4 at 719.662 kb on - strand, within espX4
Per-strain Table
Position Strand Gene LocusTag Fraction L-Arabinose remove 717,391 - plsB MCAODC_03575 0.88 -0.8 717,635 + +0.3 717,635 + +1.4 717,635 + -1.2 717,636 - -1.8 717,735 + -0.4 717,735 + +0.0 717,735 + +0.2 717,770 + +0.2 717,834 + +1.2 717,854 + -0.4 717,855 - +0.9 718,728 - -0.3 718,731 - +0.9 718,731 - +0.4 718,731 - +0.8 718,763 - +0.6 718,850 - ubiC MCAODC_03585 0.25 -0.4 718,872 - ubiC MCAODC_03585 0.29 -0.1 718,872 - ubiC MCAODC_03585 0.29 -0.2 718,872 - ubiC MCAODC_03585 0.29 -0.1 718,880 - ubiC MCAODC_03585 0.31 -1.7 718,891 - ubiC MCAODC_03585 0.33 -0.7 718,925 - ubiC MCAODC_03585 0.40 -0.5 718,925 - ubiC MCAODC_03585 0.40 -0.1 718,948 - ubiC MCAODC_03585 0.45 -0.6 718,982 - ubiC MCAODC_03585 0.51 -0.3 718,997 - ubiC MCAODC_03585 0.54 +0.1 719,178 + +0.4 719,184 - +0.6 719,184 - -0.3 719,184 - -0.0 719,184 - +0.3 719,239 - -0.8 719,243 - -0.5 719,243 - +1.0 719,247 + -0.3 719,247 + +3.0 719,248 - +0.0 719,248 - +0.8 719,248 - -0.2 719,258 + +0.0 719,275 + -0.2 719,286 + +1.0 719,286 + -3.6 719,286 + -2.2 719,297 - -0.4 719,360 + +0.6 719,361 - +2.1 719,361 - +0.6 719,447 + +1.4 719,447 + -0.4 719,447 + +0.0 719,458 - -0.9 719,467 - +1.5 719,472 + +0.0 719,473 - +1.2 719,514 + -1.5 719,514 + +0.9 719,585 + -0.0 719,585 + -1.7 719,586 - +0.7 719,586 - +0.4 719,586 - -1.5 719,586 - +2.4 719,589 + +1.1 719,590 - +1.1 719,590 - -0.1 719,591 + -0.4 719,591 + +0.9 719,591 + -0.0 719,591 + -1.5 719,591 + +1.3 719,592 - -0.0 719,592 - +0.3 719,592 - +1.7 719,592 - -0.0 719,619 + espX4 MCAODC_03590 0.11 +0.1 719,631 + espX4 MCAODC_03590 0.12 -0.8 719,631 + espX4 MCAODC_03590 0.12 -2.3 719,631 + espX4 MCAODC_03590 0.12 +0.7 719,632 - espX4 MCAODC_03590 0.12 +0.4 719,638 + espX4 MCAODC_03590 0.12 -1.4 719,639 - espX4 MCAODC_03590 0.12 -0.7 719,662 - espX4 MCAODC_03590 0.14 +0.5 719,662 - espX4 MCAODC_03590 0.14 +1.1
Or see this region's nucleotide sequence