Experiment: Bas39
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt lysB and HEPCGN_26760 overlap by 4 nucleotides HEPCGN_26760 and rrrD are separated by 1 nucleotides rrrD and HEPCGN_26770 overlap by 20 nucleotides HEPCGN_26770 and gpX are separated by 3 nucleotides gpX and HEPCGN_26780 overlap by 1 nucleotides HEPCGN_26780 and HEPCGN_26785 are separated by 95 nucleotides
HEPCGN_26755: lysB - Rz-like lysis system protein LysB, at 4,103,016 to 4,103,444
lysB
HEPCGN_26760: HEPCGN_26760 - Putative membrane protein, at 4,103,441 to 4,103,818
_26760
HEPCGN_26765: rrrD - glycoside hydrolase, at 4,103,820 to 4,104,332
rrrD
HEPCGN_26770: HEPCGN_26770 - Bacteriophage holin family HP1, at 4,104,313 to 4,104,528
_26770
HEPCGN_26775: gpX - phage tail protein, at 4,104,532 to 4,104,735
gpX
HEPCGN_26780: HEPCGN_26780 - head completion/stabilization protein, at 4,104,735 to 4,105,199
_26780
HEPCGN_26785: HEPCGN_26785 - Phage small terminase subunit, at 4,105,295 to 4,105,945
_26785
Position (kb) 4104
4105 Strain fitness (log2 ratio) -2
-1
0
1 at 4103.506 kb on - strand, within HEPCGN_26760 at 4103.518 kb on + strand, within HEPCGN_26760 at 4103.546 kb on + strand, within HEPCGN_26760 at 4103.546 kb on + strand, within HEPCGN_26760 at 4103.547 kb on - strand, within HEPCGN_26760 at 4103.547 kb on - strand, within HEPCGN_26760 at 4103.547 kb on - strand, within HEPCGN_26760 at 4103.582 kb on + strand, within HEPCGN_26760 at 4103.583 kb on - strand, within HEPCGN_26760 at 4103.781 kb on - strand at 4103.781 kb on - strand at 4103.805 kb on - strand at 4103.805 kb on - strand at 4103.836 kb on - strand at 4103.900 kb on - strand, within rrrD at 4104.092 kb on + strand, within rrrD at 4104.191 kb on - strand, within rrrD at 4104.315 kb on - strand at 4104.332 kb on + strand at 4104.332 kb on + strand at 4104.371 kb on + strand, within HEPCGN_26770 at 4104.386 kb on + strand, within HEPCGN_26770 at 4104.387 kb on - strand, within HEPCGN_26770 at 4104.387 kb on - strand, within HEPCGN_26770 at 4104.387 kb on - strand, within HEPCGN_26770 at 4104.528 kb on + strand at 4104.714 kb on - strand, within gpX at 4104.741 kb on - strand at 4104.866 kb on - strand, within HEPCGN_26780 at 4104.923 kb on - strand, within HEPCGN_26780 at 4104.923 kb on - strand, within HEPCGN_26780 at 4105.019 kb on - strand, within HEPCGN_26780
Per-strain Table
Position Strand Gene LocusTag Fraction Bas39 remove 4,103,506 - HEPCGN_26760 0.17 -1.4 4,103,518 + HEPCGN_26760 0.20 +0.6 4,103,546 + HEPCGN_26760 0.28 +0.9 4,103,546 + HEPCGN_26760 0.28 -0.1 4,103,547 - HEPCGN_26760 0.28 +0.9 4,103,547 - HEPCGN_26760 0.28 +0.1 4,103,547 - HEPCGN_26760 0.28 +0.3 4,103,582 + HEPCGN_26760 0.37 -0.7 4,103,583 - HEPCGN_26760 0.38 +0.5 4,103,781 - -1.9 4,103,781 - +0.3 4,103,805 - -0.7 4,103,805 - +0.7 4,103,836 - +0.2 4,103,900 - rrrD HEPCGN_26765 0.16 +0.0 4,104,092 + rrrD HEPCGN_26765 0.53 +1.3 4,104,191 - rrrD HEPCGN_26765 0.72 +0.5 4,104,315 - -0.8 4,104,332 + +0.1 4,104,332 + +0.1 4,104,371 + HEPCGN_26770 0.27 -0.6 4,104,386 + HEPCGN_26770 0.34 -0.5 4,104,387 - HEPCGN_26770 0.34 -1.2 4,104,387 - HEPCGN_26770 0.34 -1.8 4,104,387 - HEPCGN_26770 0.34 -0.3 4,104,528 + +0.4 4,104,714 - gpX HEPCGN_26775 0.89 +0.4 4,104,741 - +0.0 4,104,866 - HEPCGN_26780 0.28 +0.6 4,104,923 - HEPCGN_26780 0.40 +0.7 4,104,923 - HEPCGN_26780 0.40 -0.9 4,105,019 - HEPCGN_26780 0.61 -1.0
Or see this region's nucleotide sequence