Experiment: Bas39
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt icd and HEPCGN_24250 are separated by 113 nucleotides HEPCGN_24250 and tnp-IS629 are separated by 80 nucleotides tnp-IS629 and tnp-IS629 overlap by 1 nucleotides
HEPCGN_24245: icd - NADP-dependent isocitrate dehydrogenase, at 3,629,251 to 3,630,501
icd
HEPCGN_24250: HEPCGN_24250 - Integrase, at 3,630,615 to 3,631,028
_24250
HEPCGN_24255: tnp-IS629 - IS3 family IS629 transposase, at 3,631,109 to 3,631,996
tnp-IS629
HEPCGN_24260: tnp-IS629 - IS3 family IS629 transposase, at 3,631,996 to 3,632,322
tnp-IS629
Position (kb)
3630
3631
3632 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3630.495 kb on + strand at 3630.507 kb on - strand at 3630.507 kb on - strand at 3630.515 kb on + strand at 3630.516 kb on - strand at 3630.536 kb on - strand at 3630.552 kb on + strand at 3630.633 kb on + strand at 3630.634 kb on - strand at 3630.879 kb on - strand, within HEPCGN_24250 at 3630.901 kb on + strand, within HEPCGN_24250 at 3630.902 kb on - strand, within HEPCGN_24250 at 3630.933 kb on + strand, within HEPCGN_24250 at 3630.933 kb on + strand, within HEPCGN_24250 at 3630.936 kb on - strand, within HEPCGN_24250 at 3630.951 kb on - strand, within HEPCGN_24250 at 3630.954 kb on - strand, within HEPCGN_24250 at 3630.954 kb on - strand, within HEPCGN_24250 at 3631.040 kb on + strand at 3631.040 kb on + strand at 3631.041 kb on - strand at 3631.041 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bas39 remove 3,630,495 + -2.8 3,630,507 - -0.6 3,630,507 - -0.9 3,630,515 + -0.4 3,630,516 - +1.1 3,630,536 - +0.1 3,630,552 + +0.0 3,630,633 + -0.4 3,630,634 - +0.2 3,630,879 - HEPCGN_24250 0.64 -0.3 3,630,901 + HEPCGN_24250 0.69 -2.1 3,630,902 - HEPCGN_24250 0.69 +0.7 3,630,933 + HEPCGN_24250 0.77 -0.6 3,630,933 + HEPCGN_24250 0.77 -0.4 3,630,936 - HEPCGN_24250 0.78 -1.7 3,630,951 - HEPCGN_24250 0.81 -0.5 3,630,954 - HEPCGN_24250 0.82 -1.0 3,630,954 - HEPCGN_24250 0.82 -0.5 3,631,040 + +0.2 3,631,040 + +0.3 3,631,041 - -0.1 3,631,041 - -0.1
Or see this region's nucleotide sequence