Experiment: Bas39
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt recB and recD overlap by 1 nucleotides recD and argA are separated by 61 nucleotides
HEPCGN_19305: recB - exodeoxyribonuclease V subunit beta, at 2,614,410 to 2,617,952
recB
HEPCGN_19310: recD - exodeoxyribonuclease V subunit alpha, at 2,617,952 to 2,619,778
recD
HEPCGN_19315: argA - amino-acid N-acetyltransferase, at 2,619,840 to 2,621,171
argA
Position (kb)
2617
2618
2619
2620 Strain fitness (log2 ratio)
-2
-1
0
1 at 2617.001 kb on + strand, within recB at 2617.525 kb on + strand, within recB at 2617.583 kb on - strand, within recB at 2617.594 kb on + strand, within recB at 2617.638 kb on + strand at 2617.651 kb on - strand at 2617.652 kb on + strand at 2617.751 kb on + strand at 2617.752 kb on - strand at 2617.770 kb on + strand at 2617.794 kb on + strand at 2617.795 kb on - strand at 2617.827 kb on + strand at 2617.827 kb on + strand at 2617.875 kb on + strand at 2617.971 kb on + strand at 2617.999 kb on - strand at 2618.076 kb on + strand at 2618.077 kb on - strand at 2618.077 kb on - strand at 2618.273 kb on + strand, within recD at 2618.274 kb on - strand, within recD at 2618.404 kb on + strand, within recD at 2618.405 kb on - strand, within recD at 2618.405 kb on - strand, within recD at 2618.515 kb on - strand, within recD at 2618.665 kb on + strand, within recD at 2618.710 kb on - strand, within recD at 2618.897 kb on + strand, within recD at 2618.937 kb on + strand, within recD at 2618.938 kb on - strand, within recD at 2618.938 kb on - strand, within recD at 2618.938 kb on - strand, within recD at 2618.977 kb on + strand, within recD at 2618.977 kb on + strand, within recD at 2619.060 kb on + strand, within recD at 2619.163 kb on + strand, within recD at 2619.163 kb on + strand, within recD at 2619.335 kb on - strand, within recD at 2619.381 kb on - strand, within recD at 2619.433 kb on + strand, within recD at 2619.506 kb on - strand, within recD at 2619.508 kb on + strand, within recD at 2619.508 kb on + strand, within recD at 2619.565 kb on - strand, within recD at 2619.568 kb on + strand, within recD at 2619.631 kb on - strand at 2619.651 kb on - strand at 2619.651 kb on - strand at 2619.708 kb on + strand at 2619.708 kb on + strand at 2619.764 kb on - strand at 2619.782 kb on + strand at 2619.783 kb on - strand at 2619.880 kb on + strand at 2619.880 kb on + strand at 2619.881 kb on - strand at 2619.887 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bas39 remove 2,617,001 + recB HEPCGN_19305 0.73 -2.4 2,617,525 + recB HEPCGN_19305 0.88 -2.4 2,617,583 - recB HEPCGN_19305 0.90 -1.2 2,617,594 + recB HEPCGN_19305 0.90 -1.6 2,617,638 + -2.5 2,617,651 - -0.8 2,617,652 + -0.9 2,617,751 + -1.4 2,617,752 - -1.6 2,617,770 + -0.2 2,617,794 + +0.4 2,617,795 - -0.8 2,617,827 + -1.9 2,617,827 + -0.6 2,617,875 + +0.4 2,617,971 + -0.0 2,617,999 - -1.2 2,618,076 + -1.0 2,618,077 - -0.6 2,618,077 - -0.2 2,618,273 + recD HEPCGN_19310 0.18 +0.0 2,618,274 - recD HEPCGN_19310 0.18 -1.6 2,618,404 + recD HEPCGN_19310 0.25 +0.1 2,618,405 - recD HEPCGN_19310 0.25 -0.5 2,618,405 - recD HEPCGN_19310 0.25 -1.3 2,618,515 - recD HEPCGN_19310 0.31 -0.4 2,618,665 + recD HEPCGN_19310 0.39 -2.3 2,618,710 - recD HEPCGN_19310 0.41 +0.0 2,618,897 + recD HEPCGN_19310 0.52 -0.8 2,618,937 + recD HEPCGN_19310 0.54 +1.0 2,618,938 - recD HEPCGN_19310 0.54 -0.1 2,618,938 - recD HEPCGN_19310 0.54 -0.8 2,618,938 - recD HEPCGN_19310 0.54 -0.7 2,618,977 + recD HEPCGN_19310 0.56 -1.6 2,618,977 + recD HEPCGN_19310 0.56 -0.8 2,619,060 + recD HEPCGN_19310 0.61 -0.2 2,619,163 + recD HEPCGN_19310 0.66 -1.1 2,619,163 + recD HEPCGN_19310 0.66 +0.4 2,619,335 - recD HEPCGN_19310 0.76 -0.9 2,619,381 - recD HEPCGN_19310 0.78 -0.3 2,619,433 + recD HEPCGN_19310 0.81 -1.7 2,619,506 - recD HEPCGN_19310 0.85 -2.1 2,619,508 + recD HEPCGN_19310 0.85 -1.4 2,619,508 + recD HEPCGN_19310 0.85 -2.2 2,619,565 - recD HEPCGN_19310 0.88 +0.2 2,619,568 + recD HEPCGN_19310 0.88 +0.3 2,619,631 - -1.2 2,619,651 - +0.1 2,619,651 - -0.9 2,619,708 + -1.2 2,619,708 + -0.3 2,619,764 - -0.9 2,619,782 + -0.8 2,619,783 - +0.2 2,619,880 + -0.4 2,619,880 + +1.4 2,619,881 - +0.1 2,619,887 - +0.0
Or see this region's nucleotide sequence