Experiment: Bas39
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt mutH and ygdT are separated by 185 nucleotides ygdT and rppH are separated by 352 nucleotides rppH and ptsP are separated by 12 nucleotides
HEPCGN_19245: mutH - DNA mismatch repair endonuclease MutH, at 2,600,061 to 2,600,750
mutH
HEPCGN_19250: ygdT - Protein YgdT, at 2,600,936 to 2,601,082
ygdT
HEPCGN_19255: rppH - RNA pyrophosphohydrolase, at 2,601,435 to 2,601,965
rppH
HEPCGN_19260: ptsP - phosphoenolpyruvate--protein phosphotransferase, at 2,601,978 to 2,604,224
ptsP
Position (kb)
2601
2602 Strain fitness (log2 ratio)
-2
-1
0
1 at 2600.441 kb on + strand, within mutH at 2600.441 kb on + strand, within mutH at 2600.442 kb on - strand, within mutH at 2600.444 kb on + strand, within mutH at 2600.555 kb on + strand, within mutH at 2600.556 kb on - strand, within mutH at 2600.669 kb on + strand, within mutH at 2600.670 kb on - strand, within mutH at 2600.698 kb on - strand at 2600.786 kb on - strand at 2600.844 kb on - strand at 2600.992 kb on + strand, within ygdT at 2601.320 kb on - strand at 2601.351 kb on + strand at 2601.351 kb on + strand at 2601.406 kb on + strand at 2601.407 kb on - strand at 2601.407 kb on - strand at 2601.408 kb on + strand at 2601.410 kb on + strand at 2601.410 kb on + strand at 2601.410 kb on + strand at 2601.410 kb on + strand at 2601.418 kb on + strand at 2601.429 kb on - strand at 2601.467 kb on - strand at 2601.470 kb on + strand at 2601.471 kb on - strand at 2601.585 kb on + strand, within rppH at 2601.613 kb on + strand, within rppH at 2601.614 kb on - strand, within rppH at 2601.664 kb on - strand, within rppH at 2601.786 kb on - strand, within rppH at 2601.814 kb on + strand, within rppH at 2601.814 kb on + strand, within rppH at 2601.852 kb on - strand, within rppH at 2601.945 kb on + strand at 2601.946 kb on - strand at 2601.953 kb on + strand at 2601.963 kb on + strand at 2601.964 kb on - strand at 2601.964 kb on - strand at 2601.977 kb on + strand at 2601.978 kb on - strand at 2602.042 kb on + strand at 2602.047 kb on - strand at 2602.099 kb on - strand at 2602.129 kb on - strand at 2602.232 kb on - strand, within ptsP at 2602.269 kb on + strand, within ptsP at 2602.322 kb on + strand, within ptsP at 2602.348 kb on + strand, within ptsP at 2602.349 kb on - strand, within ptsP at 2602.381 kb on - strand, within ptsP at 2602.433 kb on + strand, within ptsP at 2602.433 kb on + strand, within ptsP at 2602.434 kb on - strand, within ptsP
Per-strain Table
Position Strand Gene LocusTag Fraction Bas39 remove 2,600,441 + mutH HEPCGN_19245 0.55 -0.4 2,600,441 + mutH HEPCGN_19245 0.55 +0.4 2,600,442 - mutH HEPCGN_19245 0.55 +0.2 2,600,444 + mutH HEPCGN_19245 0.56 -0.0 2,600,555 + mutH HEPCGN_19245 0.72 +0.7 2,600,556 - mutH HEPCGN_19245 0.72 -0.2 2,600,669 + mutH HEPCGN_19245 0.88 +0.6 2,600,670 - mutH HEPCGN_19245 0.88 -0.8 2,600,698 - +0.4 2,600,786 - -0.5 2,600,844 - -2.5 2,600,992 + ygdT HEPCGN_19250 0.38 +0.7 2,601,320 - +0.3 2,601,351 + -0.1 2,601,351 + +0.4 2,601,406 + -2.2 2,601,407 - +0.8 2,601,407 - -0.9 2,601,408 + +0.1 2,601,410 + +0.8 2,601,410 + +0.1 2,601,410 + +0.1 2,601,410 + +0.1 2,601,418 + -0.7 2,601,429 - -1.1 2,601,467 - -2.3 2,601,470 + -0.5 2,601,471 - -1.1 2,601,585 + rppH HEPCGN_19255 0.28 +1.7 2,601,613 + rppH HEPCGN_19255 0.34 +0.4 2,601,614 - rppH HEPCGN_19255 0.34 -1.9 2,601,664 - rppH HEPCGN_19255 0.43 -2.1 2,601,786 - rppH HEPCGN_19255 0.66 -1.2 2,601,814 + rppH HEPCGN_19255 0.71 -2.2 2,601,814 + rppH HEPCGN_19255 0.71 +0.1 2,601,852 - rppH HEPCGN_19255 0.79 -1.8 2,601,945 + -0.7 2,601,946 - -0.0 2,601,953 + +0.5 2,601,963 + +0.7 2,601,964 - -0.9 2,601,964 - -0.8 2,601,977 + +0.3 2,601,978 - -0.1 2,602,042 + +0.2 2,602,047 - +0.3 2,602,099 - +0.7 2,602,129 - +0.2 2,602,232 - ptsP HEPCGN_19260 0.11 +0.1 2,602,269 + ptsP HEPCGN_19260 0.13 -0.1 2,602,322 + ptsP HEPCGN_19260 0.15 -0.6 2,602,348 + ptsP HEPCGN_19260 0.16 +0.1 2,602,349 - ptsP HEPCGN_19260 0.17 +0.9 2,602,381 - ptsP HEPCGN_19260 0.18 +0.2 2,602,433 + ptsP HEPCGN_19260 0.20 -1.0 2,602,433 + ptsP HEPCGN_19260 0.20 +0.3 2,602,434 - ptsP HEPCGN_19260 0.20 -0.1
Or see this region's nucleotide sequence