Experiment: Bas39
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yqgE and gshB are separated by 36 nucleotides gshB and HEPCGN_18585 are separated by 12 nucleotides HEPCGN_18585 and endA are separated by 79 nucleotides endA and sprT are separated by 94 nucleotides
HEPCGN_18575: yqgE - YqgE/AlgH family protein, at 2,458,030 to 2,458,665
yqgE
HEPCGN_18580: gshB - glutathione synthase, at 2,458,702 to 2,459,652
gshB
HEPCGN_18585: HEPCGN_18585 - Ribosomal RNA small subunit methyltransferase E, at 2,459,665 to 2,460,396
_18585
HEPCGN_18590: endA - deoxyribonuclease I, at 2,460,476 to 2,461,183
endA
HEPCGN_18595: sprT - SprT family zinc-dependent metalloprotease, at 2,461,278 to 2,461,733
sprT
Position (kb)
2459
2460
2461 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2458.714 kb on + strand at 2459.012 kb on - strand, within gshB at 2459.054 kb on + strand, within gshB at 2459.176 kb on - strand, within gshB at 2459.213 kb on - strand, within gshB at 2459.223 kb on + strand, within gshB at 2459.224 kb on - strand, within gshB at 2459.232 kb on + strand, within gshB at 2459.252 kb on - strand, within gshB at 2459.286 kb on + strand, within gshB at 2459.354 kb on + strand, within gshB at 2459.354 kb on + strand, within gshB at 2459.363 kb on + strand, within gshB at 2459.364 kb on - strand, within gshB at 2459.395 kb on - strand, within gshB at 2459.465 kb on + strand, within gshB at 2459.466 kb on - strand, within gshB at 2459.541 kb on + strand, within gshB at 2459.542 kb on - strand, within gshB at 2459.542 kb on - strand, within gshB at 2459.543 kb on + strand, within gshB at 2459.543 kb on + strand, within gshB at 2459.544 kb on - strand, within gshB at 2459.544 kb on - strand, within gshB at 2459.544 kb on - strand, within gshB at 2459.544 kb on - strand, within gshB at 2459.686 kb on + strand at 2459.755 kb on - strand, within HEPCGN_18585 at 2459.761 kb on - strand, within HEPCGN_18585 at 2459.856 kb on + strand, within HEPCGN_18585 at 2459.856 kb on + strand, within HEPCGN_18585 at 2459.856 kb on + strand, within HEPCGN_18585 at 2460.086 kb on - strand, within HEPCGN_18585 at 2460.115 kb on - strand, within HEPCGN_18585 at 2460.160 kb on - strand, within HEPCGN_18585 at 2460.378 kb on - strand at 2460.396 kb on + strand at 2460.396 kb on + strand at 2460.397 kb on - strand at 2460.432 kb on - strand at 2460.472 kb on + strand at 2460.510 kb on + strand at 2460.579 kb on + strand, within endA at 2460.580 kb on - strand, within endA at 2460.580 kb on - strand, within endA at 2460.636 kb on + strand, within endA at 2460.650 kb on - strand, within endA at 2460.652 kb on - strand, within endA at 2460.652 kb on - strand, within endA at 2460.652 kb on - strand, within endA at 2460.657 kb on + strand, within endA at 2460.657 kb on + strand, within endA at 2460.657 kb on + strand, within endA at 2460.742 kb on - strand, within endA at 2460.771 kb on + strand, within endA at 2460.771 kb on + strand, within endA at 2460.771 kb on + strand, within endA at 2460.775 kb on + strand, within endA at 2460.776 kb on - strand, within endA at 2460.849 kb on + strand, within endA at 2461.028 kb on - strand, within endA at 2461.034 kb on - strand, within endA at 2461.044 kb on + strand, within endA at 2461.076 kb on + strand, within endA at 2461.111 kb on + strand, within endA at 2461.166 kb on - strand at 2461.180 kb on - strand at 2461.267 kb on - strand at 2461.318 kb on + strand at 2461.362 kb on - strand, within sprT
Per-strain Table
Position Strand Gene LocusTag Fraction Bas39 remove 2,458,714 + +0.9 2,459,012 - gshB HEPCGN_18580 0.33 -0.5 2,459,054 + gshB HEPCGN_18580 0.37 -0.4 2,459,176 - gshB HEPCGN_18580 0.50 -1.8 2,459,213 - gshB HEPCGN_18580 0.54 -0.0 2,459,223 + gshB HEPCGN_18580 0.55 -0.9 2,459,224 - gshB HEPCGN_18580 0.55 -1.4 2,459,232 + gshB HEPCGN_18580 0.56 -0.2 2,459,252 - gshB HEPCGN_18580 0.58 -0.4 2,459,286 + gshB HEPCGN_18580 0.61 -1.9 2,459,354 + gshB HEPCGN_18580 0.69 -0.1 2,459,354 + gshB HEPCGN_18580 0.69 -0.2 2,459,363 + gshB HEPCGN_18580 0.70 -3.0 2,459,364 - gshB HEPCGN_18580 0.70 -1.6 2,459,395 - gshB HEPCGN_18580 0.73 -0.8 2,459,465 + gshB HEPCGN_18580 0.80 +1.9 2,459,466 - gshB HEPCGN_18580 0.80 +0.1 2,459,541 + gshB HEPCGN_18580 0.88 -1.7 2,459,542 - gshB HEPCGN_18580 0.88 +0.0 2,459,542 - gshB HEPCGN_18580 0.88 +0.6 2,459,543 + gshB HEPCGN_18580 0.88 -0.2 2,459,543 + gshB HEPCGN_18580 0.88 -1.5 2,459,544 - gshB HEPCGN_18580 0.89 -0.8 2,459,544 - gshB HEPCGN_18580 0.89 -0.6 2,459,544 - gshB HEPCGN_18580 0.89 -1.0 2,459,544 - gshB HEPCGN_18580 0.89 -1.8 2,459,686 + +0.1 2,459,755 - HEPCGN_18585 0.12 -1.3 2,459,761 - HEPCGN_18585 0.13 -0.3 2,459,856 + HEPCGN_18585 0.26 -1.0 2,459,856 + HEPCGN_18585 0.26 -0.5 2,459,856 + HEPCGN_18585 0.26 -0.5 2,460,086 - HEPCGN_18585 0.58 +0.4 2,460,115 - HEPCGN_18585 0.61 +0.3 2,460,160 - HEPCGN_18585 0.68 -0.3 2,460,378 - +1.4 2,460,396 + -0.5 2,460,396 + -1.1 2,460,397 - +0.2 2,460,432 - -0.8 2,460,472 + -0.7 2,460,510 + -0.0 2,460,579 + endA HEPCGN_18590 0.15 +0.7 2,460,580 - endA HEPCGN_18590 0.15 +0.4 2,460,580 - endA HEPCGN_18590 0.15 +0.8 2,460,636 + endA HEPCGN_18590 0.23 -0.8 2,460,650 - endA HEPCGN_18590 0.25 -0.0 2,460,652 - endA HEPCGN_18590 0.25 -0.9 2,460,652 - endA HEPCGN_18590 0.25 -0.3 2,460,652 - endA HEPCGN_18590 0.25 -0.0 2,460,657 + endA HEPCGN_18590 0.26 +0.4 2,460,657 + endA HEPCGN_18590 0.26 -0.3 2,460,657 + endA HEPCGN_18590 0.26 -0.4 2,460,742 - endA HEPCGN_18590 0.38 +0.7 2,460,771 + endA HEPCGN_18590 0.42 -0.2 2,460,771 + endA HEPCGN_18590 0.42 +0.1 2,460,771 + endA HEPCGN_18590 0.42 +0.5 2,460,775 + endA HEPCGN_18590 0.42 +0.6 2,460,776 - endA HEPCGN_18590 0.42 -0.8 2,460,849 + endA HEPCGN_18590 0.53 +0.1 2,461,028 - endA HEPCGN_18590 0.78 -1.4 2,461,034 - endA HEPCGN_18590 0.79 +0.5 2,461,044 + endA HEPCGN_18590 0.80 -0.4 2,461,076 + endA HEPCGN_18590 0.85 -0.1 2,461,111 + endA HEPCGN_18590 0.90 +0.1 2,461,166 - +0.0 2,461,180 - +0.1 2,461,267 - +0.1 2,461,318 + -0.9 2,461,362 - sprT HEPCGN_18595 0.18 -1.0
Or see this region's nucleotide sequence