Experiment: Bas39
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt bacA and cca are separated by 180 nucleotides cca and ygiM are separated by 63 nucleotides ygiM and ygiF are separated by 241 nucleotides
HEPCGN_17560: bacA - undecaprenyl-diphosphate phosphatase, at 2,260,224 to 2,261,045
bacA
HEPCGN_17570: cca - fused tRNA nucleotidyltransferase/2',3'-cyclic phosphodiesterase/2' nucleotidase/phosphatase Cca, at 2,261,226 to 2,262,464
cca
HEPCGN_17575: ygiM - TIGR04211 family SH3 domain-containing protein, at 2,262,528 to 2,263,148
ygiM
HEPCGN_17580: ygiF - inorganic triphosphatase, at 2,263,390 to 2,264,691
ygiF
Position (kb)
2261
2262
2263 Strain fitness (log2 ratio)
-2
-1
0
1 at 2260.233 kb on - strand at 2260.313 kb on + strand, within bacA at 2260.319 kb on + strand, within bacA at 2260.320 kb on - strand, within bacA at 2260.320 kb on - strand, within bacA at 2260.406 kb on - strand, within bacA at 2260.562 kb on + strand, within bacA at 2260.593 kb on + strand, within bacA at 2260.593 kb on + strand, within bacA at 2260.594 kb on - strand, within bacA at 2260.676 kb on + strand, within bacA at 2260.676 kb on + strand, within bacA at 2260.676 kb on + strand, within bacA at 2260.683 kb on + strand, within bacA at 2260.698 kb on - strand, within bacA at 2260.792 kb on - strand, within bacA at 2260.860 kb on + strand, within bacA at 2260.917 kb on - strand, within bacA at 2260.949 kb on + strand, within bacA at 2261.002 kb on - strand at 2261.009 kb on + strand at 2261.028 kb on + strand at 2261.028 kb on + strand at 2261.028 kb on + strand at 2261.029 kb on - strand at 2261.054 kb on + strand at 2261.054 kb on + strand at 2261.055 kb on - strand at 2261.180 kb on + strand at 2261.767 kb on - strand, within cca at 2262.502 kb on + strand at 2262.503 kb on - strand at 2262.623 kb on - strand, within ygiM at 2262.626 kb on + strand, within ygiM at 2262.626 kb on + strand, within ygiM at 2262.626 kb on + strand, within ygiM at 2262.627 kb on - strand, within ygiM at 2262.627 kb on - strand, within ygiM at 2262.627 kb on - strand, within ygiM at 2262.669 kb on + strand, within ygiM at 2262.670 kb on - strand, within ygiM at 2262.739 kb on - strand, within ygiM at 2262.949 kb on + strand, within ygiM at 2262.973 kb on - strand, within ygiM at 2262.973 kb on - strand, within ygiM at 2262.973 kb on - strand, within ygiM at 2262.973 kb on - strand, within ygiM at 2262.975 kb on + strand, within ygiM at 2262.975 kb on + strand, within ygiM at 2263.049 kb on + strand, within ygiM at 2263.050 kb on - strand, within ygiM at 2263.069 kb on + strand, within ygiM at 2263.116 kb on + strand at 2263.117 kb on - strand at 2263.161 kb on - strand at 2263.311 kb on + strand at 2263.314 kb on + strand at 2263.319 kb on - strand at 2263.319 kb on - strand at 2263.319 kb on - strand at 2263.319 kb on - strand at 2263.363 kb on - strand at 2263.372 kb on - strand at 2263.382 kb on + strand at 2263.383 kb on - strand at 2263.416 kb on + strand at 2263.417 kb on - strand at 2263.423 kb on - strand at 2263.461 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bas39 remove 2,260,233 - +0.7 2,260,313 + bacA HEPCGN_17560 0.11 +0.1 2,260,319 + bacA HEPCGN_17560 0.12 -0.2 2,260,320 - bacA HEPCGN_17560 0.12 -0.2 2,260,320 - bacA HEPCGN_17560 0.12 -0.4 2,260,406 - bacA HEPCGN_17560 0.22 -0.5 2,260,562 + bacA HEPCGN_17560 0.41 +0.5 2,260,593 + bacA HEPCGN_17560 0.45 +0.5 2,260,593 + bacA HEPCGN_17560 0.45 -0.0 2,260,594 - bacA HEPCGN_17560 0.45 -0.9 2,260,676 + bacA HEPCGN_17560 0.55 -0.5 2,260,676 + bacA HEPCGN_17560 0.55 +0.0 2,260,676 + bacA HEPCGN_17560 0.55 +0.6 2,260,683 + bacA HEPCGN_17560 0.56 +0.9 2,260,698 - bacA HEPCGN_17560 0.58 +0.4 2,260,792 - bacA HEPCGN_17560 0.69 +0.7 2,260,860 + bacA HEPCGN_17560 0.77 -0.7 2,260,917 - bacA HEPCGN_17560 0.84 -1.3 2,260,949 + bacA HEPCGN_17560 0.88 -0.4 2,261,002 - +0.3 2,261,009 + -0.2 2,261,028 + +0.3 2,261,028 + -2.2 2,261,028 + +0.4 2,261,029 - +0.2 2,261,054 + -0.9 2,261,054 + -0.2 2,261,055 - -0.2 2,261,180 + -0.5 2,261,767 - cca HEPCGN_17570 0.44 -1.4 2,262,502 + +0.4 2,262,503 - -0.1 2,262,623 - ygiM HEPCGN_17575 0.15 +0.3 2,262,626 + ygiM HEPCGN_17575 0.16 -0.5 2,262,626 + ygiM HEPCGN_17575 0.16 -0.7 2,262,626 + ygiM HEPCGN_17575 0.16 +0.9 2,262,627 - ygiM HEPCGN_17575 0.16 -0.1 2,262,627 - ygiM HEPCGN_17575 0.16 +0.2 2,262,627 - ygiM HEPCGN_17575 0.16 -1.8 2,262,669 + ygiM HEPCGN_17575 0.23 -0.0 2,262,670 - ygiM HEPCGN_17575 0.23 -0.1 2,262,739 - ygiM HEPCGN_17575 0.34 +0.3 2,262,949 + ygiM HEPCGN_17575 0.68 +0.1 2,262,973 - ygiM HEPCGN_17575 0.72 +0.7 2,262,973 - ygiM HEPCGN_17575 0.72 +0.8 2,262,973 - ygiM HEPCGN_17575 0.72 -0.4 2,262,973 - ygiM HEPCGN_17575 0.72 -0.8 2,262,975 + ygiM HEPCGN_17575 0.72 +0.3 2,262,975 + ygiM HEPCGN_17575 0.72 +0.1 2,263,049 + ygiM HEPCGN_17575 0.84 -0.1 2,263,050 - ygiM HEPCGN_17575 0.84 +1.6 2,263,069 + ygiM HEPCGN_17575 0.87 +0.3 2,263,116 + +0.7 2,263,117 - -0.2 2,263,161 - +0.4 2,263,311 + +0.3 2,263,314 + +0.6 2,263,319 - +0.2 2,263,319 - +0.2 2,263,319 - -0.2 2,263,319 - +0.1 2,263,363 - +0.6 2,263,372 - -2.3 2,263,382 + +0.6 2,263,383 - -0.7 2,263,416 + -0.2 2,263,417 - -0.2 2,263,423 - -0.5 2,263,461 + -0.2
Or see this region's nucleotide sequence