Experiment: Bas39
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt asd and yhgO are separated by 73 nucleotides yhgO and glgB are separated by 157 nucleotides
HEPCGN_15690: asd - aspartate-semialdehyde dehydrogenase, at 1,900,654 to 1,901,757
asd
HEPCGN_15695: yhgO - Protein YhgO, at 1,901,831 to 1,901,872
yhgO
HEPCGN_15700: glgB - 1,4-alpha-glucan branching enzyme, at 1,902,030 to 1,904,216
glgB
Position (kb)
1901
1902 Strain fitness (log2 ratio)
-1
0
1
2 at 1901.762 kb on + strand at 1901.769 kb on + strand at 1901.770 kb on - strand at 1901.804 kb on + strand at 1901.807 kb on + strand at 1901.807 kb on + strand at 1901.808 kb on - strand at 1901.816 kb on - strand at 1901.847 kb on + strand, within yhgO at 1901.870 kb on + strand at 1901.884 kb on + strand at 1901.885 kb on - strand at 1901.892 kb on + strand at 1901.893 kb on - strand at 1902.012 kb on + strand at 1902.013 kb on - strand at 1902.068 kb on - strand at 1902.113 kb on + strand at 1902.114 kb on - strand at 1902.149 kb on - strand at 1902.419 kb on + strand, within glgB at 1902.419 kb on + strand, within glgB at 1902.419 kb on + strand, within glgB at 1902.422 kb on + strand, within glgB at 1902.422 kb on + strand, within glgB at 1902.422 kb on + strand, within glgB at 1902.422 kb on + strand, within glgB at 1902.422 kb on + strand, within glgB at 1902.423 kb on - strand, within glgB at 1902.441 kb on - strand, within glgB at 1902.441 kb on - strand, within glgB at 1902.502 kb on - strand, within glgB at 1902.593 kb on + strand, within glgB at 1902.593 kb on + strand, within glgB at 1902.594 kb on - strand, within glgB at 1902.597 kb on + strand, within glgB at 1902.823 kb on - strand, within glgB at 1902.853 kb on - strand, within glgB at 1902.853 kb on - strand, within glgB at 1902.857 kb on + strand, within glgB
Per-strain Table
Position Strand Gene LocusTag Fraction Bas39 remove 1,901,762 + +0.0 1,901,769 + -0.7 1,901,770 - +0.4 1,901,804 + +0.9 1,901,807 + +0.6 1,901,807 + -0.1 1,901,808 - +0.5 1,901,816 - -0.2 1,901,847 + yhgO HEPCGN_15695 0.38 -0.8 1,901,870 + -0.3 1,901,884 + +0.6 1,901,885 - +1.1 1,901,892 + +0.3 1,901,893 - -1.4 1,902,012 + +0.0 1,902,013 - +0.6 1,902,068 - +0.3 1,902,113 + +0.6 1,902,114 - -0.6 1,902,149 - +0.2 1,902,419 + glgB HEPCGN_15700 0.18 +0.1 1,902,419 + glgB HEPCGN_15700 0.18 -0.0 1,902,419 + glgB HEPCGN_15700 0.18 -1.2 1,902,422 + glgB HEPCGN_15700 0.18 +1.9 1,902,422 + glgB HEPCGN_15700 0.18 -0.4 1,902,422 + glgB HEPCGN_15700 0.18 -1.1 1,902,422 + glgB HEPCGN_15700 0.18 -0.1 1,902,422 + glgB HEPCGN_15700 0.18 +0.3 1,902,423 - glgB HEPCGN_15700 0.18 +0.5 1,902,441 - glgB HEPCGN_15700 0.19 +0.0 1,902,441 - glgB HEPCGN_15700 0.19 +0.8 1,902,502 - glgB HEPCGN_15700 0.22 +0.2 1,902,593 + glgB HEPCGN_15700 0.26 +0.0 1,902,593 + glgB HEPCGN_15700 0.26 +0.3 1,902,594 - glgB HEPCGN_15700 0.26 -0.3 1,902,597 + glgB HEPCGN_15700 0.26 +0.3 1,902,823 - glgB HEPCGN_15700 0.36 +0.6 1,902,853 - glgB HEPCGN_15700 0.38 -0.0 1,902,853 - glgB HEPCGN_15700 0.38 +0.7 1,902,857 + glgB HEPCGN_15700 0.38 +0.2
Or see this region's nucleotide sequence