Experiment: Bas39
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rfaJ and HEPCGN_14525 are separated by 78 nucleotides HEPCGN_14525 and HEPCGN_14530 are separated by 45 nucleotides
HEPCGN_14520: rfaJ - UDP-galactose--(galactosyl) LPS alpha1,2-galactosyltransferase, at 1,656,991 to 1,658,019
rfaJ
HEPCGN_14525: HEPCGN_14525 - beta1,3-glucosyltransferase, at 1,658,098 to 1,659,081
_14525
HEPCGN_14530: HEPCGN_14530 - O-antigen ligase domain-containing protein, at 1,659,127 to 1,660,380
_14530
Position (kb)
1658
1659
1660 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1657.113 kb on + strand, within rfaJ at 1657.114 kb on - strand, within rfaJ at 1657.213 kb on - strand, within rfaJ at 1657.363 kb on + strand, within rfaJ at 1657.852 kb on + strand, within rfaJ at 1658.233 kb on - strand, within HEPCGN_14525 at 1658.338 kb on - strand, within HEPCGN_14525 at 1658.363 kb on + strand, within HEPCGN_14525 at 1658.452 kb on + strand, within HEPCGN_14525 at 1658.476 kb on - strand, within HEPCGN_14525 at 1658.477 kb on + strand, within HEPCGN_14525 at 1658.504 kb on + strand, within HEPCGN_14525 at 1658.673 kb on - strand, within HEPCGN_14525 at 1658.719 kb on - strand, within HEPCGN_14525 at 1658.800 kb on + strand, within HEPCGN_14525 at 1659.014 kb on + strand at 1659.108 kb on + strand at 1659.182 kb on + strand at 1659.183 kb on - strand at 1659.444 kb on + strand, within HEPCGN_14530 at 1659.461 kb on + strand, within HEPCGN_14530 at 1659.732 kb on - strand, within HEPCGN_14530 at 1659.744 kb on - strand, within HEPCGN_14530 at 1660.078 kb on - strand, within HEPCGN_14530
Per-strain Table
Position Strand Gene LocusTag Fraction Bas39 remove 1,657,113 + rfaJ HEPCGN_14520 0.12 -1.0 1,657,114 - rfaJ HEPCGN_14520 0.12 -2.5 1,657,213 - rfaJ HEPCGN_14520 0.22 -1.9 1,657,363 + rfaJ HEPCGN_14520 0.36 -0.7 1,657,852 + rfaJ HEPCGN_14520 0.84 -1.2 1,658,233 - HEPCGN_14525 0.14 -2.8 1,658,338 - HEPCGN_14525 0.24 -4.2 1,658,363 + HEPCGN_14525 0.27 -3.9 1,658,452 + HEPCGN_14525 0.36 -2.1 1,658,476 - HEPCGN_14525 0.38 +0.8 1,658,477 + HEPCGN_14525 0.39 -2.7 1,658,504 + HEPCGN_14525 0.41 -3.7 1,658,673 - HEPCGN_14525 0.58 -2.3 1,658,719 - HEPCGN_14525 0.63 -0.2 1,658,800 + HEPCGN_14525 0.71 -1.9 1,659,014 + -3.1 1,659,108 + -0.3 1,659,182 + -2.0 1,659,183 - -2.6 1,659,444 + HEPCGN_14530 0.25 -2.0 1,659,461 + HEPCGN_14530 0.27 -1.0 1,659,732 - HEPCGN_14530 0.48 -2.6 1,659,744 - HEPCGN_14530 0.49 -1.4 1,660,078 - HEPCGN_14530 0.76 -1.7
Or see this region's nucleotide sequence