Experiment: Bas39
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt recG and trmH are separated by 5 nucleotides trmH and spoT are separated by 6 nucleotides spoT and rpoZ are separated by 18 nucleotides rpoZ and gmk are separated by 54 nucleotides
HEPCGN_14365: recG - ATP-dependent DNA helicase RecG, at 1,629,478 to 1,631,559
recG
HEPCGN_14370: trmH - tRNA (guanosine(18)-2'-O)-methyltransferase TrmH, at 1,631,565 to 1,632,254
trmH
HEPCGN_14375: spoT - bifunctional GTP diphosphokinase/guanosine-3',5'-bis pyrophosphate 3'-pyrophosphohydrolase, at 1,632,261 to 1,634,369
spoT
HEPCGN_14380: rpoZ - DNA-directed RNA polymerase subunit omega, at 1,634,388 to 1,634,663
rpoZ
HEPCGN_14385: gmk - guanylate kinase, at 1,634,718 to 1,635,341
gmk
Position (kb)
1632
1633
1634
1635 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1631.343 kb on + strand, within recG at 1631.343 kb on + strand, within recG at 1631.344 kb on - strand, within recG at 1631.344 kb on - strand, within recG at 1631.379 kb on - strand at 1631.392 kb on + strand at 1631.393 kb on - strand at 1631.447 kb on - strand at 1631.543 kb on - strand at 1631.743 kb on + strand, within trmH at 1631.743 kb on + strand, within trmH at 1631.910 kb on - strand, within trmH at 1632.054 kb on - strand, within trmH at 1632.121 kb on - strand, within trmH at 1632.230 kb on + strand at 1632.231 kb on - strand at 1632.231 kb on - strand at 1632.231 kb on - strand at 1632.231 kb on - strand at 1632.254 kb on + strand at 1632.285 kb on - strand at 1632.285 kb on - strand at 1632.285 kb on - strand at 1632.320 kb on + strand at 1632.320 kb on + strand at 1632.371 kb on - strand at 1632.428 kb on + strand at 1632.428 kb on + strand at 1632.429 kb on - strand at 1632.429 kb on - strand at 1632.516 kb on - strand, within spoT at 1632.624 kb on - strand, within spoT at 1634.521 kb on - strand, within rpoZ at 1634.605 kb on - strand, within rpoZ at 1634.653 kb on - strand at 1634.664 kb on - strand at 1634.667 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bas39 remove 1,631,343 + recG HEPCGN_14365 0.90 -1.0 1,631,343 + recG HEPCGN_14365 0.90 -0.2 1,631,344 - recG HEPCGN_14365 0.90 -0.3 1,631,344 - recG HEPCGN_14365 0.90 -0.1 1,631,379 - -0.4 1,631,392 + -0.5 1,631,393 - -0.4 1,631,447 - -0.5 1,631,543 - +0.1 1,631,743 + trmH HEPCGN_14370 0.26 -0.1 1,631,743 + trmH HEPCGN_14370 0.26 -1.2 1,631,910 - trmH HEPCGN_14370 0.50 +0.1 1,632,054 - trmH HEPCGN_14370 0.71 -0.9 1,632,121 - trmH HEPCGN_14370 0.81 -0.1 1,632,230 + -3.1 1,632,231 - -0.7 1,632,231 - +0.9 1,632,231 - -0.3 1,632,231 - -0.3 1,632,254 + -0.2 1,632,285 - -0.6 1,632,285 - -2.4 1,632,285 - -1.0 1,632,320 + -1.3 1,632,320 + -1.3 1,632,371 - +0.4 1,632,428 + -2.2 1,632,428 + +0.2 1,632,429 - -0.5 1,632,429 - +0.5 1,632,516 - spoT HEPCGN_14375 0.12 -0.4 1,632,624 - spoT HEPCGN_14375 0.17 -0.1 1,634,521 - rpoZ HEPCGN_14380 0.48 -1.1 1,634,605 - rpoZ HEPCGN_14380 0.79 -0.2 1,634,653 - -0.8 1,634,664 - +0.0 1,634,667 + -1.0
Or see this region's nucleotide sequence