Strain Fitness in Escherichia coli ECOR38 around HEPCGN_14375

Experiment: Bas39

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntrecG and trmH are separated by 5 nucleotidestrmH and spoT are separated by 6 nucleotidesspoT and rpoZ are separated by 18 nucleotidesrpoZ and gmk are separated by 54 nucleotides HEPCGN_14365: recG - ATP-dependent DNA helicase RecG, at 1,629,478 to 1,631,559 recG HEPCGN_14370: trmH - tRNA (guanosine(18)-2'-O)-methyltransferase TrmH, at 1,631,565 to 1,632,254 trmH HEPCGN_14375: spoT - bifunctional GTP diphosphokinase/guanosine-3',5'-bis pyrophosphate 3'-pyrophosphohydrolase, at 1,632,261 to 1,634,369 spoT HEPCGN_14380: rpoZ - DNA-directed RNA polymerase subunit omega, at 1,634,388 to 1,634,663 rpoZ HEPCGN_14385: gmk - guanylate kinase, at 1,634,718 to 1,635,341 gmk Position (kb) 1632 1633 1634 1635Strain fitness (log2 ratio) -3 -2 -1 0 1at 1631.343 kb on + strand, within recGat 1631.343 kb on + strand, within recGat 1631.344 kb on - strand, within recGat 1631.344 kb on - strand, within recGat 1631.379 kb on - strandat 1631.392 kb on + strandat 1631.393 kb on - strandat 1631.447 kb on - strandat 1631.543 kb on - strandat 1631.743 kb on + strand, within trmHat 1631.743 kb on + strand, within trmHat 1631.910 kb on - strand, within trmHat 1632.054 kb on - strand, within trmHat 1632.121 kb on - strand, within trmHat 1632.230 kb on + strandat 1632.231 kb on - strandat 1632.231 kb on - strandat 1632.231 kb on - strandat 1632.231 kb on - strandat 1632.254 kb on + strandat 1632.285 kb on - strandat 1632.285 kb on - strandat 1632.285 kb on - strandat 1632.320 kb on + strandat 1632.320 kb on + strandat 1632.371 kb on - strandat 1632.428 kb on + strandat 1632.428 kb on + strandat 1632.429 kb on - strandat 1632.429 kb on - strandat 1632.516 kb on - strand, within spoTat 1632.624 kb on - strand, within spoTat 1634.521 kb on - strand, within rpoZat 1634.605 kb on - strand, within rpoZat 1634.653 kb on - strandat 1634.664 kb on - strandat 1634.667 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas39
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1,631,343 + recG HEPCGN_14365 0.90 -1.0
1,631,343 + recG HEPCGN_14365 0.90 -0.2
1,631,344 - recG HEPCGN_14365 0.90 -0.3
1,631,344 - recG HEPCGN_14365 0.90 -0.1
1,631,379 - -0.4
1,631,392 + -0.5
1,631,393 - -0.4
1,631,447 - -0.5
1,631,543 - +0.1
1,631,743 + trmH HEPCGN_14370 0.26 -0.1
1,631,743 + trmH HEPCGN_14370 0.26 -1.2
1,631,910 - trmH HEPCGN_14370 0.50 +0.1
1,632,054 - trmH HEPCGN_14370 0.71 -0.9
1,632,121 - trmH HEPCGN_14370 0.81 -0.1
1,632,230 + -3.1
1,632,231 - -0.7
1,632,231 - +0.9
1,632,231 - -0.3
1,632,231 - -0.3
1,632,254 + -0.2
1,632,285 - -0.6
1,632,285 - -2.4
1,632,285 - -1.0
1,632,320 + -1.3
1,632,320 + -1.3
1,632,371 - +0.4
1,632,428 + -2.2
1,632,428 + +0.2
1,632,429 - -0.5
1,632,429 - +0.5
1,632,516 - spoT HEPCGN_14375 0.12 -0.4
1,632,624 - spoT HEPCGN_14375 0.17 -0.1
1,634,521 - rpoZ HEPCGN_14380 0.48 -1.1
1,634,605 - rpoZ HEPCGN_14380 0.79 -0.2
1,634,653 - -0.8
1,634,664 - +0.0
1,634,667 + -1.0

Or see this region's nucleotide sequence