Experiment: Bas39
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt guaC and yacM are separated by 155 nucleotides yacM and coaE are separated by 24 nucleotides coaE and zapD overlap by 1 nucleotides zapD and yacG are separated by 9 nucleotides yacG and mutT are separated by 87 nucleotides mutT and secA are separated by 59 nucleotides
HEPCGN_08530: guaC - GMP reductase, at 448,027 to 449,070
guaC
HEPCGN_08535: yacM - Protein YacM, at 449,226 to 449,270
yacM
HEPCGN_08540: coaE - dephospho-CoA kinase, at 449,295 to 449,915
coaE
HEPCGN_08545: zapD - cell division protein ZapD, at 449,915 to 450,658
zapD
HEPCGN_08550: yacG - DNA gyrase inhibitor YacG, at 450,668 to 450,865
yacG
HEPCGN_08560: mutT - 8-oxo-dGTP diphosphatase MutT, at 450,953 to 451,351
mutT
HEPCGN_08570: secA - preprotein translocase subunit SecA, at 451,411 to 454,116
secA
Position (kb) 449
450
451 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 449.001 kb on + strand at 449.038 kb on - strand at 449.095 kb on + strand at 449.095 kb on + strand at 449.131 kb on + strand at 449.131 kb on + strand at 449.131 kb on + strand at 449.131 kb on + strand at 449.132 kb on - strand at 449.205 kb on - strand at 449.205 kb on - strand at 449.208 kb on + strand at 449.305 kb on + strand at 449.306 kb on - strand at 449.306 kb on - strand at 449.306 kb on - strand at 449.946 kb on + strand at 449.962 kb on + strand at 449.962 kb on + strand at 450.010 kb on - strand, within zapD at 450.131 kb on - strand, within zapD at 450.131 kb on - strand, within zapD at 450.321 kb on + strand, within zapD at 450.322 kb on - strand, within zapD at 450.348 kb on + strand, within zapD at 450.418 kb on + strand, within zapD at 450.418 kb on + strand, within zapD at 450.418 kb on + strand, within zapD at 450.419 kb on - strand, within zapD at 450.435 kb on + strand, within zapD at 450.436 kb on - strand, within zapD at 450.569 kb on - strand, within zapD at 450.569 kb on - strand, within zapD at 450.569 kb on - strand, within zapD at 450.680 kb on - strand at 450.680 kb on - strand at 450.772 kb on - strand, within yacG at 450.871 kb on + strand at 450.872 kb on - strand at 450.924 kb on + strand at 450.924 kb on + strand at 450.925 kb on - strand at 450.963 kb on + strand at 450.963 kb on + strand at 450.964 kb on - strand at 450.964 kb on - strand at 450.973 kb on + strand at 450.973 kb on + strand at 450.974 kb on - strand at 451.112 kb on + strand, within mutT at 451.112 kb on + strand, within mutT at 451.113 kb on - strand, within mutT at 451.113 kb on - strand, within mutT at 451.113 kb on - strand, within mutT at 451.113 kb on - strand, within mutT at 451.113 kb on - strand, within mutT at 451.114 kb on + strand, within mutT at 451.114 kb on + strand, within mutT at 451.114 kb on + strand, within mutT at 451.114 kb on + strand, within mutT at 451.115 kb on - strand, within mutT at 451.115 kb on - strand, within mutT at 451.135 kb on - strand, within mutT at 451.292 kb on + strand, within mutT at 451.294 kb on + strand, within mutT at 451.295 kb on - strand, within mutT at 451.324 kb on + strand at 451.324 kb on + strand at 451.357 kb on + strand at 451.357 kb on + strand at 451.358 kb on - strand at 451.393 kb on + strand at 451.413 kb on - strand at 451.413 kb on - strand at 451.620 kb on + strand at 451.620 kb on + strand at 451.620 kb on + strand at 451.621 kb on - strand at 451.621 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bas39 remove 449,001 + +0.1 449,038 - +1.1 449,095 + -0.1 449,095 + +1.2 449,131 + -0.1 449,131 + -0.1 449,131 + +0.2 449,131 + +0.6 449,132 - +1.5 449,205 - -0.6 449,205 - -3.5 449,208 + -0.8 449,305 + +0.9 449,306 - -0.5 449,306 - -1.7 449,306 - +1.0 449,946 + +0.2 449,962 + -0.6 449,962 + +0.4 450,010 - zapD HEPCGN_08545 0.13 -0.2 450,131 - zapD HEPCGN_08545 0.29 +0.1 450,131 - zapD HEPCGN_08545 0.29 -1.7 450,321 + zapD HEPCGN_08545 0.55 -0.5 450,322 - zapD HEPCGN_08545 0.55 -0.5 450,348 + zapD HEPCGN_08545 0.58 -1.1 450,418 + zapD HEPCGN_08545 0.68 -0.6 450,418 + zapD HEPCGN_08545 0.68 -0.8 450,418 + zapD HEPCGN_08545 0.68 -1.4 450,419 - zapD HEPCGN_08545 0.68 +0.2 450,435 + zapD HEPCGN_08545 0.70 -0.0 450,436 - zapD HEPCGN_08545 0.70 -0.3 450,569 - zapD HEPCGN_08545 0.88 -1.8 450,569 - zapD HEPCGN_08545 0.88 -0.1 450,569 - zapD HEPCGN_08545 0.88 -0.6 450,680 - +0.1 450,680 - -0.1 450,772 - yacG HEPCGN_08550 0.53 -0.0 450,871 + +0.5 450,872 - +0.2 450,924 + +0.4 450,924 + +0.5 450,925 - +0.4 450,963 + +0.2 450,963 + +0.4 450,964 - +0.3 450,964 - -0.4 450,973 + -1.2 450,973 + -0.0 450,974 - -1.1 451,112 + mutT HEPCGN_08560 0.40 +0.7 451,112 + mutT HEPCGN_08560 0.40 -2.1 451,113 - mutT HEPCGN_08560 0.40 -1.0 451,113 - mutT HEPCGN_08560 0.40 -0.2 451,113 - mutT HEPCGN_08560 0.40 +0.5 451,113 - mutT HEPCGN_08560 0.40 +0.6 451,113 - mutT HEPCGN_08560 0.40 -0.2 451,114 + mutT HEPCGN_08560 0.40 -0.1 451,114 + mutT HEPCGN_08560 0.40 +0.2 451,114 + mutT HEPCGN_08560 0.40 -0.3 451,114 + mutT HEPCGN_08560 0.40 +0.7 451,115 - mutT HEPCGN_08560 0.41 +0.9 451,115 - mutT HEPCGN_08560 0.41 +0.2 451,135 - mutT HEPCGN_08560 0.46 -0.2 451,292 + mutT HEPCGN_08560 0.85 +0.4 451,294 + mutT HEPCGN_08560 0.85 -0.1 451,295 - mutT HEPCGN_08560 0.86 -1.0 451,324 + +0.1 451,324 + -0.1 451,357 + +0.2 451,357 + +0.4 451,358 - +0.4 451,393 + -0.0 451,413 - -0.5 451,413 - -0.3 451,620 + +1.2 451,620 + -0.4 451,620 + -2.0 451,621 - +0.2 451,621 - -0.2
Or see this region's nucleotide sequence