Experiment: Bas39
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt gluQRS and pcnB are separated by 92 nucleotides pcnB and folK overlap by 4 nucleotides folK and yadN are separated by 370 nucleotides
HEPCGN_08300: gluQRS - tRNA glutamyl-Q(34) synthetase GluQRS, at 398,848 to 399,774
gluQRS
HEPCGN_08305: pcnB - polynucleotide adenylyltransferase PcnB, at 399,867 to 401,231
pcnB
HEPCGN_08310: folK - 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine diphosphokinase, at 401,228 to 401,707
folK
HEPCGN_08315: yadN - Uncharacterized fimbrial-like protein YadN, at 402,078 to 402,662
yadN
Position (kb) 399
400
401
402 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 398.893 kb on + strand at 398.893 kb on + strand at 398.894 kb on - strand at 398.896 kb on - strand at 398.966 kb on - strand, within gluQRS at 398.969 kb on + strand, within gluQRS at 399.003 kb on - strand, within gluQRS at 399.008 kb on + strand, within gluQRS at 399.008 kb on + strand, within gluQRS at 399.152 kb on + strand, within gluQRS at 399.153 kb on - strand, within gluQRS at 399.154 kb on + strand, within gluQRS at 399.167 kb on + strand, within gluQRS at 399.167 kb on + strand, within gluQRS at 399.167 kb on + strand, within gluQRS at 399.168 kb on - strand, within gluQRS at 399.168 kb on - strand, within gluQRS at 399.168 kb on - strand, within gluQRS at 399.173 kb on - strand, within gluQRS at 399.173 kb on - strand, within gluQRS at 399.198 kb on + strand, within gluQRS at 399.198 kb on + strand, within gluQRS at 399.199 kb on - strand, within gluQRS at 399.220 kb on + strand, within gluQRS at 399.314 kb on + strand, within gluQRS at 399.315 kb on - strand, within gluQRS at 399.360 kb on + strand, within gluQRS at 399.363 kb on + strand, within gluQRS at 399.363 kb on + strand, within gluQRS at 399.391 kb on + strand, within gluQRS at 399.391 kb on + strand, within gluQRS at 399.392 kb on - strand, within gluQRS at 399.394 kb on - strand, within gluQRS at 399.520 kb on + strand, within gluQRS at 399.521 kb on - strand, within gluQRS at 399.521 kb on - strand, within gluQRS at 399.650 kb on + strand, within gluQRS at 399.718 kb on - strand at 399.777 kb on + strand at 399.872 kb on - strand at 399.872 kb on - strand at 399.947 kb on + strand at 399.947 kb on + strand at 399.947 kb on + strand at 399.947 kb on + strand at 399.948 kb on - strand at 399.948 kb on - strand at 399.962 kb on + strand at 399.963 kb on - strand at 399.985 kb on + strand at 399.986 kb on - strand at 399.990 kb on + strand at 400.051 kb on + strand, within pcnB at 400.220 kb on + strand, within pcnB at 400.262 kb on + strand, within pcnB at 400.262 kb on + strand, within pcnB at 400.263 kb on - strand, within pcnB at 400.338 kb on + strand, within pcnB at 400.546 kb on + strand, within pcnB at 400.620 kb on + strand, within pcnB at 400.627 kb on + strand, within pcnB at 400.627 kb on + strand, within pcnB at 400.627 kb on + strand, within pcnB at 400.627 kb on + strand, within pcnB at 400.627 kb on + strand, within pcnB at 400.627 kb on + strand, within pcnB at 400.628 kb on - strand, within pcnB at 400.628 kb on - strand, within pcnB at 400.638 kb on + strand, within pcnB at 400.639 kb on - strand, within pcnB at 400.940 kb on - strand, within pcnB at 401.018 kb on + strand, within pcnB at 401.034 kb on + strand, within pcnB at 401.183 kb on + strand at 401.674 kb on + strand at 402.043 kb on + strand at 402.167 kb on - strand, within yadN
Per-strain Table
Position Strand Gene LocusTag Fraction Bas39 remove 398,893 + +0.3 398,893 + -0.8 398,894 - -0.1 398,896 - +0.7 398,966 - gluQRS HEPCGN_08300 0.13 -0.8 398,969 + gluQRS HEPCGN_08300 0.13 -1.8 399,003 - gluQRS HEPCGN_08300 0.17 +0.2 399,008 + gluQRS HEPCGN_08300 0.17 -0.4 399,008 + gluQRS HEPCGN_08300 0.17 +0.2 399,152 + gluQRS HEPCGN_08300 0.33 -0.1 399,153 - gluQRS HEPCGN_08300 0.33 +0.9 399,154 + gluQRS HEPCGN_08300 0.33 -0.5 399,167 + gluQRS HEPCGN_08300 0.34 -0.3 399,167 + gluQRS HEPCGN_08300 0.34 +1.1 399,167 + gluQRS HEPCGN_08300 0.34 +0.1 399,168 - gluQRS HEPCGN_08300 0.35 +0.2 399,168 - gluQRS HEPCGN_08300 0.35 -0.3 399,168 - gluQRS HEPCGN_08300 0.35 -0.3 399,173 - gluQRS HEPCGN_08300 0.35 +3.1 399,173 - gluQRS HEPCGN_08300 0.35 +0.2 399,198 + gluQRS HEPCGN_08300 0.38 +0.2 399,198 + gluQRS HEPCGN_08300 0.38 -0.3 399,199 - gluQRS HEPCGN_08300 0.38 +1.5 399,220 + gluQRS HEPCGN_08300 0.40 +0.1 399,314 + gluQRS HEPCGN_08300 0.50 +0.2 399,315 - gluQRS HEPCGN_08300 0.50 +0.3 399,360 + gluQRS HEPCGN_08300 0.55 +0.8 399,363 + gluQRS HEPCGN_08300 0.56 +0.8 399,363 + gluQRS HEPCGN_08300 0.56 +0.2 399,391 + gluQRS HEPCGN_08300 0.59 +0.2 399,391 + gluQRS HEPCGN_08300 0.59 +3.4 399,392 - gluQRS HEPCGN_08300 0.59 +0.7 399,394 - gluQRS HEPCGN_08300 0.59 -0.1 399,520 + gluQRS HEPCGN_08300 0.72 +0.1 399,521 - gluQRS HEPCGN_08300 0.73 +0.0 399,521 - gluQRS HEPCGN_08300 0.73 +0.3 399,650 + gluQRS HEPCGN_08300 0.87 +0.4 399,718 - -0.2 399,777 + -0.0 399,872 - -1.7 399,872 - -0.5 399,947 + -0.4 399,947 + +0.2 399,947 + -0.6 399,947 + -1.0 399,948 - -2.5 399,948 - -1.5 399,962 + +0.6 399,963 - -0.5 399,985 + +1.0 399,986 - -0.0 399,990 + +0.3 400,051 + pcnB HEPCGN_08305 0.13 -1.7 400,220 + pcnB HEPCGN_08305 0.26 -0.9 400,262 + pcnB HEPCGN_08305 0.29 -0.0 400,262 + pcnB HEPCGN_08305 0.29 -1.3 400,263 - pcnB HEPCGN_08305 0.29 +0.7 400,338 + pcnB HEPCGN_08305 0.35 -0.3 400,546 + pcnB HEPCGN_08305 0.50 -0.4 400,620 + pcnB HEPCGN_08305 0.55 -0.4 400,627 + pcnB HEPCGN_08305 0.56 -0.6 400,627 + pcnB HEPCGN_08305 0.56 -0.9 400,627 + pcnB HEPCGN_08305 0.56 -0.6 400,627 + pcnB HEPCGN_08305 0.56 -0.4 400,627 + pcnB HEPCGN_08305 0.56 -1.1 400,627 + pcnB HEPCGN_08305 0.56 -0.5 400,628 - pcnB HEPCGN_08305 0.56 -1.7 400,628 - pcnB HEPCGN_08305 0.56 -1.8 400,638 + pcnB HEPCGN_08305 0.56 -0.1 400,639 - pcnB HEPCGN_08305 0.57 -2.8 400,940 - pcnB HEPCGN_08305 0.79 -0.2 401,018 + pcnB HEPCGN_08305 0.84 +0.7 401,034 + pcnB HEPCGN_08305 0.85 +0.3 401,183 + +0.3 401,674 + -1.3 402,043 + -0.7 402,167 - yadN HEPCGN_08315 0.15 +0.3
Or see this region's nucleotide sequence